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Organisms Acellular Viruses Cellular Prokaryotes Eukaryotes The Cell Theory All living organisms are composed of cells. They may be unicellular or multicellular. The cell is the structural and functional unit of life. Cells arise from pre-existing cells. All cells have the same basic chemical composition. Basic features of all cells Plasma membrane Semifluid substance called cytosol Organelles Genetic material The organelle is a specialized subunit within a cell that has a specific function. Non-membrane bound organelles: ribosomes, centrioles, cytoskeleton, chromosomes Single-membrane: endoplasmic reticulum (rough and smooth), Golgi apparatus, lysosomes, peroxisomes, vacuoles Double-membrane: nucleus, mitochondrion, plastid (chloroplast) Ribosomes Ribosomes are composed of RNAs and proteins. Ribosomes are found in cytoplasm, on rough ER, in mitochondria and plastids. Their function is protein synthesis. Centrioles Centrioles are made of 9 triplets of microtubules that are arranged in a ring. They form spindle fibers during cell division. Cytoskeleton Maintain and change cell shape, move organelles within the cell Chromosomes The function of chromosomes is to carry hereditary information The mitochondrion The site of cellular respiration and energy production The Nucleus a control center of the cell because it contains genetic material Endoplasmic reticulum Network of membranous channels Synthesis of proteins, carbohydrates and lipids and steroids and their transport Golgi apparatus Modification, sorting and packaging of proteins. Lysosomes formation. Lysosomes Membrane vesicles containing hydrolytic proteins (digestive enzymes). Breakdown of large molecules (proteins, lipids, nucleic acids, carbohydrates) Peroxisomes Membrane vesicles containing oxidative enzymes. The enzymes break down molecules and as result produce hydrogen peroxide molecules. Hydrogen peroxide is immediately converted by the enzyme called catalase to water and oxygen. Prokaryotic cell Absence of internal membrane-bound organelles!!! Plasma membrane Fluid mosaic model (Nicolson and Singer, 1972) Plasma membrane encloses cell and cell organelles Made of hydrophobic and hydrophilic components Semi-permeable and fluid-like Components lipid bilayer Sterols stabilize the lipid bilayer. Integral (transmembrane) proteins penetrate the hydrophobic core. Peripheral (exterior) proteins are bound on the surface of the membrane. Carbohydrates as part of glycolipids and glycoproteins. Functions Separating intracellular components from environment To maintain homeostasis within the cell (a stable internal environment) Transport (passage) of materials into or out of the cell. The cell membrane has selective permeability. Signal transduction (reception of information) by cell surface receptors . Cell-cell recognition. Physical connection with other cells. Transport Passive transport: materials move from high concentration to low concentration and doesn’t require energy. Active transport: molecules move through the membrane from areas of low concentration to areas of high concentration with the help of energy. It uses energy to move solutes against their gradients. Passive transport • Simple diffusion: small molecules (gases such as oxygen, carbon dioxide, water) diffuses across a membrane from the region of higher concentration to the region of lower concentration. • Facilitated diffusion: big and/or polar molecules (ions through channel proteins, sugars, amino acids by transmembrane transporters; aquaporins for facilitated diffusion of water). Active transport • Ion pumps (sodium potassium pump). • Endocytosis: the membrane encircles and engulfs a food particle. The membranes fuse together, forming a vesicle. If the material brought into the cell is solid – phagocytosis, liquid – pinocytosis. • Exocytosis is the reverse of endocytosis.