Lipoprotein Lipase in the Arterial Wall
... The role of apolipoproteins in the interaction between LPL and triglyceride-rich lipoproteins during hydrolysis of triglycerides has been studied extensively. Efficient hydrolysis of triglyceride-rich lipoproteins depends on 1 of the several apolipoproteins present on the surface of these particles, ...
... The role of apolipoproteins in the interaction between LPL and triglyceride-rich lipoproteins during hydrolysis of triglycerides has been studied extensively. Efficient hydrolysis of triglyceride-rich lipoproteins depends on 1 of the several apolipoproteins present on the surface of these particles, ...
Donohoe, B.S., B. - University of Colorado-MCDB
... Analysis of high-pressure frozen/freeze-substituted cells via electron tomography has generated critical data in support of the cisternal progression/maturation model of Golgi trafficking primarily in the form of high resolution, 3D morphological information on the Golgi apparatus and associated mem ...
... Analysis of high-pressure frozen/freeze-substituted cells via electron tomography has generated critical data in support of the cisternal progression/maturation model of Golgi trafficking primarily in the form of high resolution, 3D morphological information on the Golgi apparatus and associated mem ...
University of Groningen The functional relationship between
... intestinal lumen. The bile salt/phospholipid mixed-micelles take up fat-soluble compounds and carry them through the intestinal tract so they can be absorbed in the gut or efficiently secreted in the feces (6). At the terminal ileum, the majority (~95%) of bile salts is reabsorbed and return to the ...
... intestinal lumen. The bile salt/phospholipid mixed-micelles take up fat-soluble compounds and carry them through the intestinal tract so they can be absorbed in the gut or efficiently secreted in the feces (6). At the terminal ileum, the majority (~95%) of bile salts is reabsorbed and return to the ...
Lipopolysaccharide-bound structure of the antimicrobial peptide
... The use of antibiotics has led to the emergence of multidrug-resistant bacteria, resulting in untreatable infections and nosocomial infections. Antimicrobial peptides (AMPs), components of the innate immune system, are being investigated as potential therapeutics to replace or complement traditional ...
... The use of antibiotics has led to the emergence of multidrug-resistant bacteria, resulting in untreatable infections and nosocomial infections. Antimicrobial peptides (AMPs), components of the innate immune system, are being investigated as potential therapeutics to replace or complement traditional ...
Interactions of Annexins with the mu Subunits of the Clathrin
... To search for proteins that interact with the N-terminal domain of annexin A2, the N-terminal 34 residues were fused to the yeast GAL4 DNA binding domain and used as a bait in a yeast two-hybrid screen of a human fetal brain cDNA library. The bait construct was found not to cause autoactivation in c ...
... To search for proteins that interact with the N-terminal domain of annexin A2, the N-terminal 34 residues were fused to the yeast GAL4 DNA binding domain and used as a bait in a yeast two-hybrid screen of a human fetal brain cDNA library. The bait construct was found not to cause autoactivation in c ...
Myosin V Plays an Essential Role in the Thyroid Hormone
... in the absence and presence of T4. No differences in total actin content were observed, and ⬎90% of the immunoreactive actin was found in the Triton-insoluble pellet in both thyroid hormone-deficient and T4-treated cells as determined by densitometry (data not shown). In control experiments, no spec ...
... in the absence and presence of T4. No differences in total actin content were observed, and ⬎90% of the immunoreactive actin was found in the Triton-insoluble pellet in both thyroid hormone-deficient and T4-treated cells as determined by densitometry (data not shown). In control experiments, no spec ...
The mechanism of Stx2 enrichment in outer membrane vesicles of
... virulence factors produced by non-obligate pathogens have a function for survival in the environment. Since the majority of the time these microbes are living without a mammalian host, it is likely that Stx1 has a function not involved with mammalian pathogenesis. Specific Aim 1: Test the hypothesis ...
... virulence factors produced by non-obligate pathogens have a function for survival in the environment. Since the majority of the time these microbes are living without a mammalian host, it is likely that Stx1 has a function not involved with mammalian pathogenesis. Specific Aim 1: Test the hypothesis ...
Carbohydrate Biosensors
... carbohydrate biosensors field and point to possible future avenues. We have not attempted to cover all aspects of carbohydrate chemistry and biology, carbohydrate-detection methods, or issues concerning molecular processes involving carbohydrates; these aspects are broad and prolific fields of study ...
... carbohydrate biosensors field and point to possible future avenues. We have not attempted to cover all aspects of carbohydrate chemistry and biology, carbohydrate-detection methods, or issues concerning molecular processes involving carbohydrates; these aspects are broad and prolific fields of study ...
Regulation of ryanodine receptors from skeletal and cardiac muscle
... Calcium sparks once provided a ray of hope for understanding cardiac EC coupling. It is now understood that the graded response of Ca2+ release in cells is due to recruitment of different numbers of regenerative Ca2+ release events that are localised at the triad junctions. These release events are ...
... Calcium sparks once provided a ray of hope for understanding cardiac EC coupling. It is now understood that the graded response of Ca2+ release in cells is due to recruitment of different numbers of regenerative Ca2+ release events that are localised at the triad junctions. These release events are ...
Annexin-Phospholipid Interactions. Functional Implications
... Inside each domain, a sequence of 17 amino acid residues is found which presents an even higher degree of conservation. Annexin A5 was the first member of this family to be crystallized and its structure to be studied by X-ray diffraction [6,7]. Since then, several other annexins have been crystalli ...
... Inside each domain, a sequence of 17 amino acid residues is found which presents an even higher degree of conservation. Annexin A5 was the first member of this family to be crystallized and its structure to be studied by X-ray diffraction [6,7]. Since then, several other annexins have been crystalli ...
CONTRIBUTION OF DYSFERLIN-CONTAINING
... or environmental factors such as activity level [19, 23]. Mild cardiac involvement can occasionally be detected in dysferlinopathy patients, but overt cardiomyopathy due to dysferlin-deficiency is rare [24]. Dysferlinopathy patients share several common pathological features with non-dysferlin muscu ...
... or environmental factors such as activity level [19, 23]. Mild cardiac involvement can occasionally be detected in dysferlinopathy patients, but overt cardiomyopathy due to dysferlin-deficiency is rare [24]. Dysferlinopathy patients share several common pathological features with non-dysferlin muscu ...
Compare TIRF Geometries
... living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.” [Steyer JA, Almers W. A real-time view of life within 100 nm of the plasma membrane. Nat Rev Mol Cell Biol. 2001, 2(4), 268]. TIRF systems can be implemented in different ...
... living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.” [Steyer JA, Almers W. A real-time view of life within 100 nm of the plasma membrane. Nat Rev Mol Cell Biol. 2001, 2(4), 268]. TIRF systems can be implemented in different ...
Nanoscale Architecture of Endoplasmic Reticulum Export Sites and
... chemical fixation and staining protocols that are now known to produce major changes in the architecture of ER and Golgi membranes. In particular, the harsh chemical reaction conditions used during zinc iodine osmium staining to generate distinctive but membranedistorting stain deposits in ER and Go ...
... chemical fixation and staining protocols that are now known to produce major changes in the architecture of ER and Golgi membranes. In particular, the harsh chemical reaction conditions used during zinc iodine osmium staining to generate distinctive but membranedistorting stain deposits in ER and Go ...
A three-stage model of Golgi structure and function
... unusual, because most carbohydrate synthesis reactions could occur efficiently in a single mixed compartment (Dunphy and Rothman 1985). A more general explanation for the cisternal structure of the Golgi is that this organelle serves as a ‘‘delay timer’’ (Glick and Malhotra 1998). Biosynthetic cargo ...
... unusual, because most carbohydrate synthesis reactions could occur efficiently in a single mixed compartment (Dunphy and Rothman 1985). A more general explanation for the cisternal structure of the Golgi is that this organelle serves as a ‘‘delay timer’’ (Glick and Malhotra 1998). Biosynthetic cargo ...
Virus Research Influenza virus morphogenesis and budding
... also support this notion. On the other hand, specific nucleotide sequences of different RNA segments have been shown to favor packaging into virions (Liang et al., 2008; Marsh et al., 2007, 2008), but not for assembly and bud release. Indeed, virus replication can be abortive at many stages of the vi ...
... also support this notion. On the other hand, specific nucleotide sequences of different RNA segments have been shown to favor packaging into virions (Liang et al., 2008; Marsh et al., 2007, 2008), but not for assembly and bud release. Indeed, virus replication can be abortive at many stages of the vi ...
Biogenesis and multifaceted roles of outer membrane
... increases their efficacy. Furthermore, if multiple enzymes are needed for a distant activity, for example degradation of complex molecules from a solid surface, then OMVs can co-transport proteins so that they reach the remote site simultaneously. Thus, the energy expenditure of the cell in making O ...
... increases their efficacy. Furthermore, if multiple enzymes are needed for a distant activity, for example degradation of complex molecules from a solid surface, then OMVs can co-transport proteins so that they reach the remote site simultaneously. Thus, the energy expenditure of the cell in making O ...
How mammals sense infection: from endotoxin to the Toll-like receptors Bruce Beutler
... Never again was it necessary to make a BAC contig or search for genes. ...
... Never again was it necessary to make a BAC contig or search for genes. ...
Functional Domains within Fusion Proteins: Prospectives for
... could also self- and co-assemble with the WT fusion peptide in the membrane. Interestingly, the WT, but not the V2E mutant, induced liposome aggregation, destabilization, and fusion. Moreover, the V2E mutant inhibited vesicle fusion induced by the WT peptide, probably by forming inactive heteroaggre ...
... could also self- and co-assemble with the WT fusion peptide in the membrane. Interestingly, the WT, but not the V2E mutant, induced liposome aggregation, destabilization, and fusion. Moreover, the V2E mutant inhibited vesicle fusion induced by the WT peptide, probably by forming inactive heteroaggre ...
Rapid RNA Exchange in Aqueous Two-Phase System and Coacervate Droplets Christian Hentrich
... occurred orders of magnitude more rapidly than across the membrane of fatty acid vesicles. Although our FRAP measurements report only on the entry of RNA oligomers into ATPS or coacervate droplets, at steady state, the rate of efflux of RNA from droplets must equal the rate of influx. Our data there ...
... occurred orders of magnitude more rapidly than across the membrane of fatty acid vesicles. Although our FRAP measurements report only on the entry of RNA oligomers into ATPS or coacervate droplets, at steady state, the rate of efflux of RNA from droplets must equal the rate of influx. Our data there ...
original version
... phospholipids appears to change as cells pass from exponential growth into stationary phase [51,52] and recent work shows that depletion of cellular acidic phospholipids leads to under initiation of replication from oriC during the cell-cycle [39]. Acidic phospholipids, particularly CL, are present ...
... phospholipids appears to change as cells pass from exponential growth into stationary phase [51,52] and recent work shows that depletion of cellular acidic phospholipids leads to under initiation of replication from oriC during the cell-cycle [39]. Acidic phospholipids, particularly CL, are present ...
Full-Text PDF
... phospholipids appears to change as cells pass from exponential growth into stationary phase [51,52] and recent work shows that depletion of cellular acidic phospholipids leads to under initiation of replication from oriC during the cell-cycle [39]. Acidic phospholipids, particularly CL, are present ...
... phospholipids appears to change as cells pass from exponential growth into stationary phase [51,52] and recent work shows that depletion of cellular acidic phospholipids leads to under initiation of replication from oriC during the cell-cycle [39]. Acidic phospholipids, particularly CL, are present ...
Comparison of the structure in human skin and acquired
... The cornified layer consists of 10-20 piled up layers of SG1 dead cells and a single corneocyte can be 10-30 µm in diameter and 1 µm thick (11). The cornified layer does not consist of homogenous corneocyte cells within its layer and there is evidence that indicates that the cornified layer has thre ...
... The cornified layer consists of 10-20 piled up layers of SG1 dead cells and a single corneocyte can be 10-30 µm in diameter and 1 µm thick (11). The cornified layer does not consist of homogenous corneocyte cells within its layer and there is evidence that indicates that the cornified layer has thre ...
Electron tomography of plant thylakoid membranes
... understood. Electron cryo-tomography (cryo-ET) is a powerful new technique for visualizing cellular structures, especially membranes, in three dimensions. By this technique, large membrane protein complexes, such as the photosystem II supercomplex or the chloroplast ATP synthase, can be visualized d ...
... understood. Electron cryo-tomography (cryo-ET) is a powerful new technique for visualizing cellular structures, especially membranes, in three dimensions. By this technique, large membrane protein complexes, such as the photosystem II supercomplex or the chloroplast ATP synthase, can be visualized d ...
Presequence-Independent Manner Transported by the TIM23
... mitochondria, many multispanning inner membrane proteins, such as the metabolite carriers and channel-forming subunits of the inner membrane protein translocases, utilize internal targeting signals. The transport of multispanning proteins into the inner membrane has been studied most for metabolite ...
... mitochondria, many multispanning inner membrane proteins, such as the metabolite carriers and channel-forming subunits of the inner membrane protein translocases, utilize internal targeting signals. The transport of multispanning proteins into the inner membrane has been studied most for metabolite ...
Model lipid bilayer
A model lipid bilayer is any bilayer assembled in vitro, as opposed to the bilayer of natural cell membranes or covering various sub-cellular structures like the nucleus. A model bilayer can be made with either synthetic or natural lipids. The simplest model systems contain only a single pure synthetic lipid. More physiologically relevant model bilayers can be made with mixtures of several synthetic or natural lipids.There are many different types of model bilayers, each having experimental advantages and disadvantages. The first system developed was the black lipid membrane or “painted” bilayer, which allows simple electrical characterization of bilayers but is short-lived and can be difficult to work with. Supported bilayers are anchored to a solid substrate, increasing stability and allowing the use of characterization tools not possible in bulk solution. These advantages come at the cost of unwanted substrate interactions which can denature membrane proteins.