University of Groningen Cross-linking of dimeric CitS and GltS
... Lolkema 1996, Sobczak and Lolkema 2003). pBADCitS encodes the CitS protein extended with a N-terminal His6-tag and an enterokinase cleavage site. pSB260 is a pBlueScript II (Stratagene) derivative containing the citS gene with the gene encoding BAD inserted in a NcoI restriction site at a position c ...
... Lolkema 1996, Sobczak and Lolkema 2003). pBADCitS encodes the CitS protein extended with a N-terminal His6-tag and an enterokinase cleavage site. pSB260 is a pBlueScript II (Stratagene) derivative containing the citS gene with the gene encoding BAD inserted in a NcoI restriction site at a position c ...
a b
... These authors contribute equally to this work. 0196-9781/$ – see front matter ß 2008 Elsevier Inc. All rights reserved. doi:10.1016/j.peptides.2008.12.002 ...
... These authors contribute equally to this work. 0196-9781/$ – see front matter ß 2008 Elsevier Inc. All rights reserved. doi:10.1016/j.peptides.2008.12.002 ...
Dynamics of lipoprotein transport in the human circulatory system
... The secreted human LPL protein has 448 amino acids. It is functional as a dimer. LPL is a member of a triacylglycerol lipase protein family (Chapter 10) others of which include hepatic lipase, which like LPL is released into the plasma by heparin, and pancreatic lipase. Pancreatic lipase and several ...
... The secreted human LPL protein has 448 amino acids. It is functional as a dimer. LPL is a member of a triacylglycerol lipase protein family (Chapter 10) others of which include hepatic lipase, which like LPL is released into the plasma by heparin, and pancreatic lipase. Pancreatic lipase and several ...
Facing extremes: archaeal surface-layer (glyco)proteins
... survival in the face of such environmental challenges. One aspect of such investigation asks how the archaeal cell envelope, directly exposed to the harsh physical conditions in which these micro-organisms exist, manages to maintain its structural integrity. In most cases, a surface (S)-layer, gener ...
... survival in the face of such environmental challenges. One aspect of such investigation asks how the archaeal cell envelope, directly exposed to the harsh physical conditions in which these micro-organisms exist, manages to maintain its structural integrity. In most cases, a surface (S)-layer, gener ...
Chloroplast envelope membranes: a dynamic interface between
... contain only phospholipids: mostly PC and PE). Therefore, determination of the glycerolipid composition of isolated plant membranes is a good way to probe their purity. In plants grown under normal conditions, galactolipids are restricted to plastid membranes. However, in the past decade, it has bee ...
... contain only phospholipids: mostly PC and PE). Therefore, determination of the glycerolipid composition of isolated plant membranes is a good way to probe their purity. In plants grown under normal conditions, galactolipids are restricted to plastid membranes. However, in the past decade, it has bee ...
Formation of Helical Hairpins during Membrane Protein Integration
... The helical hairpin, two closely spaced transmembrane helices separated by a short turn, is a common structural element in integral membrane proteins. Previous studies on the sequence determinants of helical hairpin formation have focussed on the role of polar and charged residues placed centrally i ...
... The helical hairpin, two closely spaced transmembrane helices separated by a short turn, is a common structural element in integral membrane proteins. Previous studies on the sequence determinants of helical hairpin formation have focussed on the role of polar and charged residues placed centrally i ...
The role of hyaluronic acid in protecting surface
... to inhibit PLA2 activity, and without such protection SAPL become accessible to hydrolysis (Fig. 4b and c). It is warranted to assume that, in these circumstances, HA not only loses its role as a protector of phospholipids but also increases their hydrolysis by PLA2. It is well known that under cert ...
... to inhibit PLA2 activity, and without such protection SAPL become accessible to hydrolysis (Fig. 4b and c). It is warranted to assume that, in these circumstances, HA not only loses its role as a protector of phospholipids but also increases their hydrolysis by PLA2. It is well known that under cert ...
S C T
... membrane domains composed of two or more TM α-helices usually consisting of a stretch of about 15-30 amino acids with a central hydrophobic domain flanked by more polar residues (Wallin et al., 1997). The loops connecting two TM αhelices can be as short as three residues (Bowie, 1997). The hydrophob ...
... membrane domains composed of two or more TM α-helices usually consisting of a stretch of about 15-30 amino acids with a central hydrophobic domain flanked by more polar residues (Wallin et al., 1997). The loops connecting two TM αhelices can be as short as three residues (Bowie, 1997). The hydrophob ...
Membrane immersion allows rhomboid proteases to achieve
... Site-2 proteases are metalloenzymes that release transcription factors from the membrane to regulate membrane biogenesis and stress responses in diverse organisms from bacterial pathogens to man (Rawson et al., 1997; Makinoshima and Glickman, 2005). Lastly, rhomboid proteases act as prime regulators ...
... Site-2 proteases are metalloenzymes that release transcription factors from the membrane to regulate membrane biogenesis and stress responses in diverse organisms from bacterial pathogens to man (Rawson et al., 1997; Makinoshima and Glickman, 2005). Lastly, rhomboid proteases act as prime regulators ...
Glycosyltransferases and Transpeptidases/Penicillin-Binding
... Abstract: Peptidoglycan (PG) is an essential macromolecular sacculus surrounding most bacteria. It is assembled by the glycosyltransferase (GT) and transpeptidase (TP) activities of multimodular penicillin-binding proteins (PBPs) within multiprotein complex machineries. Both activities are essential ...
... Abstract: Peptidoglycan (PG) is an essential macromolecular sacculus surrounding most bacteria. It is assembled by the glycosyltransferase (GT) and transpeptidase (TP) activities of multimodular penicillin-binding proteins (PBPs) within multiprotein complex machineries. Both activities are essential ...
A painful TR(i)P to lysosomes
... size and their distribution in the cell is highly regulated (Xu and Ren, 2015; Li et al., 2016). A recent study suggests that peripherally localized lysosomes are less acidic (Johnson et al., 2016). Shang et al. (2016) used line-scan confocal imaging to show that Ca2+ release was from individual Lam ...
... size and their distribution in the cell is highly regulated (Xu and Ren, 2015; Li et al., 2016). A recent study suggests that peripherally localized lysosomes are less acidic (Johnson et al., 2016). Shang et al. (2016) used line-scan confocal imaging to show that Ca2+ release was from individual Lam ...
A painful TR(i)P to lysosomes
... size and their distribution in the cell is highly regulated (Xu and Ren, 2015; Li et al., 2016). A recent study suggests that peripherally localized lysosomes are less acidic (Johnson et al., 2016). Shang et al. (2016) used line-scan confocal imaging to show that Ca2+ release was from individual Lam ...
... size and their distribution in the cell is highly regulated (Xu and Ren, 2015; Li et al., 2016). A recent study suggests that peripherally localized lysosomes are less acidic (Johnson et al., 2016). Shang et al. (2016) used line-scan confocal imaging to show that Ca2+ release was from individual Lam ...
A painful TR(i)P to lysosomes
... size and their distribution in the cell is highly regulated (Xu and Ren, 2015; Li et al., 2016). A recent study suggests that peripherally localized lysosomes are less acidic (Johnson et al., 2016). Shang et al. (2016) used line-scan confocal imaging to show that Ca2+ release was from individual Lam ...
... size and their distribution in the cell is highly regulated (Xu and Ren, 2015; Li et al., 2016). A recent study suggests that peripherally localized lysosomes are less acidic (Johnson et al., 2016). Shang et al. (2016) used line-scan confocal imaging to show that Ca2+ release was from individual Lam ...
A painful TR(i)P to lysosomes
... size and their distribution in the cell is highly regulated (Xu and Ren, 2015; Li et al., 2016). A recent study suggests that peripherally localized lysosomes are less acidic (Johnson et al., 2016). Shang et al. (2016) used line-scan confocal imaging to show that Ca2+ release was from individual Lam ...
... size and their distribution in the cell is highly regulated (Xu and Ren, 2015; Li et al., 2016). A recent study suggests that peripherally localized lysosomes are less acidic (Johnson et al., 2016). Shang et al. (2016) used line-scan confocal imaging to show that Ca2+ release was from individual Lam ...
Progress in the Correlative Atomic Force Microscopy and Optical
... between molecules at nanometer resolution. However, AFM cannot provide the precise information of synchronized molecular groups and has many shortcomings in the aspects of determining the mechanism of the interactions and the elaborate structure due to the limitations of the technology, itself, such ...
... between molecules at nanometer resolution. However, AFM cannot provide the precise information of synchronized molecular groups and has many shortcomings in the aspects of determining the mechanism of the interactions and the elaborate structure due to the limitations of the technology, itself, such ...
Triglyceride-Rich Lipoproteins as Agents of Innate Immunity
... low-density lipoproteins could also bind LPS in much the same way [11]. Previous studies examining a potential interaction between triglyceride-rich lipoproteins and LPS had shown that very-low-density lipoproteins had much less ability to interact and form complexes with LPS than did the cholestero ...
... low-density lipoproteins could also bind LPS in much the same way [11]. Previous studies examining a potential interaction between triglyceride-rich lipoproteins and LPS had shown that very-low-density lipoproteins had much less ability to interact and form complexes with LPS than did the cholestero ...
Rapid Colorimetric Detection of Antibody
... general applicability of the system for detection of epitopeantibody recognition. The CR values recorded for each epitopeL7A7K4G/DMPC/PDA assembly indicate that pronounced colorimetric transitions occur only when the displayed epitopes are recognized by their specific antibodies. The color changes i ...
... general applicability of the system for detection of epitopeantibody recognition. The CR values recorded for each epitopeL7A7K4G/DMPC/PDA assembly indicate that pronounced colorimetric transitions occur only when the displayed epitopes are recognized by their specific antibodies. The color changes i ...
- Journal of Hepatology
... component of VLDLs. It can dissociate from the VLDL surface to rapidly associate with HDLs and promote discoidal particle morphology [11]. ApoC-I has multiple regulatory actions in plasma TRL metabolism including inhibiting the binding and/or uptake of VLDL by LDL-R and LRP. This is believed to be d ...
... component of VLDLs. It can dissociate from the VLDL surface to rapidly associate with HDLs and promote discoidal particle morphology [11]. ApoC-I has multiple regulatory actions in plasma TRL metabolism including inhibiting the binding and/or uptake of VLDL by LDL-R and LRP. This is believed to be d ...
6 Colloidal dispersion
... germicides & not cause irritation as ionic silver salts. 2) Colloidal copper used in cancer. 3) Colloidal gold used as diagnostic agent. 4) Colloidal mercury used in syphilis. 5) Association colloids (SAA) are used to increase solubility & stability of c ...
... germicides & not cause irritation as ionic silver salts. 2) Colloidal copper used in cancer. 3) Colloidal gold used as diagnostic agent. 4) Colloidal mercury used in syphilis. 5) Association colloids (SAA) are used to increase solubility & stability of c ...
ENS’06 FUSION PHAGE AS A BIOSELECTIVE NANOMATERIAL: EVOLUTION OF THE CONCEPT
... or mixed with Texas Red-conjugated streptavidin (TRS), washed and analyzed by fluorescent microscopy and FACS. Complex of the modified Doxil with the target streptavidin demonstrated 50-fold higher fluorescence than pure Doxil and 10-fold higher fluorescence than control Doxil treated with TRS, as r ...
... or mixed with Texas Red-conjugated streptavidin (TRS), washed and analyzed by fluorescent microscopy and FACS. Complex of the modified Doxil with the target streptavidin demonstrated 50-fold higher fluorescence than pure Doxil and 10-fold higher fluorescence than control Doxil treated with TRS, as r ...
Increased Susceptibility of the Sickle Cell Membrane
... Coomassie-stained gel, it was apparent that a large band (HMWP present at the top of the gel) composed of polymerized membrane proteins, formed during treatment with tBHP. The HMWP band was evident at 10 minutes and became distinct at 30 minutes. This band formed in both normal and sickle cell membr ...
... Coomassie-stained gel, it was apparent that a large band (HMWP present at the top of the gel) composed of polymerized membrane proteins, formed during treatment with tBHP. The HMWP band was evident at 10 minutes and became distinct at 30 minutes. This band formed in both normal and sickle cell membr ...
PHOSPHOLIPIDS AND BIOMEMBRANES L. L. M. VAN DEENEN
... the recovery of the lipids in this material to a significant extent, while on the ...
... the recovery of the lipids in this material to a significant extent, while on the ...
Structural features of the vesicle of Frankia sp. CpI1
... and show internal differentiation. The shape of the vesicles within nodule cells of different hosts is under the control of the host cells (Lalonde 1978). The vesicles as well as the rest of the Frankia filaments within the nodule cells are surrounded by and "shaped" by the polysaccharide encapsulat ...
... and show internal differentiation. The shape of the vesicles within nodule cells of different hosts is under the control of the host cells (Lalonde 1978). The vesicles as well as the rest of the Frankia filaments within the nodule cells are surrounded by and "shaped" by the polysaccharide encapsulat ...
Mitofilin complexes: conserved organizers of mitochondrial
... contact sites was first proposed by Hackenbrock 1968. In this study, isolated mitochondria were diluted into a buffer of high osmolarity, which leads to the extrusion of water and shrinking of the mitochondrial matrix compartment. Electron microscopy images showed that, under these conditions, the i ...
... contact sites was first proposed by Hackenbrock 1968. In this study, isolated mitochondria were diluted into a buffer of high osmolarity, which leads to the extrusion of water and shrinking of the mitochondrial matrix compartment. Electron microscopy images showed that, under these conditions, the i ...
Model lipid bilayer
A model lipid bilayer is any bilayer assembled in vitro, as opposed to the bilayer of natural cell membranes or covering various sub-cellular structures like the nucleus. A model bilayer can be made with either synthetic or natural lipids. The simplest model systems contain only a single pure synthetic lipid. More physiologically relevant model bilayers can be made with mixtures of several synthetic or natural lipids.There are many different types of model bilayers, each having experimental advantages and disadvantages. The first system developed was the black lipid membrane or “painted” bilayer, which allows simple electrical characterization of bilayers but is short-lived and can be difficult to work with. Supported bilayers are anchored to a solid substrate, increasing stability and allowing the use of characterization tools not possible in bulk solution. These advantages come at the cost of unwanted substrate interactions which can denature membrane proteins.