Lipid raft–associated protein sorting in exosomes
... transferrin receptors (TfRs) during reticulocyte maturation.8 On the other hand, exosomes secreted by antigen-presenting cells (APCs) contain few TfRs, but are enriched with major histocompatibility complex (MHC) class II molecules,3,4 which has led to investigations of their possible implication in ...
... transferrin receptors (TfRs) during reticulocyte maturation.8 On the other hand, exosomes secreted by antigen-presenting cells (APCs) contain few TfRs, but are enriched with major histocompatibility complex (MHC) class II molecules,3,4 which has led to investigations of their possible implication in ...
Connection of the Mitochondrial Outer and Inner Membranes by
... the ⌬fzo1 deletion mutant which is rho0, that is, it lacks mitochondrial DNA (Rapaport et al., 1998). This strain was crossed with its isogenic wild-type strain YPH500 (Sikorski and Hieter, 1989). The resulting diploid strain was sporulated and tetrads were dissected. One rho⫹ clone was isolated tha ...
... the ⌬fzo1 deletion mutant which is rho0, that is, it lacks mitochondrial DNA (Rapaport et al., 1998). This strain was crossed with its isogenic wild-type strain YPH500 (Sikorski and Hieter, 1989). The resulting diploid strain was sporulated and tetrads were dissected. One rho⫹ clone was isolated tha ...
In-Cell Solid-State NMR - Institut de Biologie Physico
... (1), and 15 years later the focus shifted from looking at water molecules to examining lipids (2) and small metabolites (3). In the 2000s, larger soluble proteins within bacterial cells were studied by NMR (4), and in-cell NMR became a field in and of itself, with investigators identifying common ob ...
... (1), and 15 years later the focus shifted from looking at water molecules to examining lipids (2) and small metabolites (3). In the 2000s, larger soluble proteins within bacterial cells were studied by NMR (4), and in-cell NMR became a field in and of itself, with investigators identifying common ob ...
REVIEWS
... The acquisition of multidrug resistance is a serious impediment to improved healthcare. Multidrug resistance is most frequently due to active transporters that pump a broad spectrum of chemically distinct, cytotoxic molecules out of cells, including antibiotics, antimalarials, herbicides and cancer ...
... The acquisition of multidrug resistance is a serious impediment to improved healthcare. Multidrug resistance is most frequently due to active transporters that pump a broad spectrum of chemically distinct, cytotoxic molecules out of cells, including antibiotics, antimalarials, herbicides and cancer ...
Bugging the cell wall of bacteria
... the focus on the therapeutic potential of other Lipid II targeting compounds. Glycopeptide analogues containing an additional hydrophobic moiety, such as telavancin and oritavancin, have shown great promise in overcoming vancomycin resistance [30]. Another highly active antibacterial compound, ramop ...
... the focus on the therapeutic potential of other Lipid II targeting compounds. Glycopeptide analogues containing an additional hydrophobic moiety, such as telavancin and oritavancin, have shown great promise in overcoming vancomycin resistance [30]. Another highly active antibacterial compound, ramop ...
Phosphatidylinositol 4-Phosphate Formation at ER Exit Sites
... formation step (Rowe et al., 1996). Indeed, ARF1 is not present in the vesicle formation assay reconstituted with purified COPII coat components under conditions where full inhibition of ER export by Fapp1-PH is observed (Figure 2A). We did not observe specific interactions between Sar1 and any of t ...
... formation step (Rowe et al., 1996). Indeed, ARF1 is not present in the vesicle formation assay reconstituted with purified COPII coat components under conditions where full inhibition of ER export by Fapp1-PH is observed (Figure 2A). We did not observe specific interactions between Sar1 and any of t ...
Transformations of phosphatidylinositol phosphates in the outer and
... (PIP1s) in Golgi [16,17]. These incongruities in the composition of the membrane continuum brought us to speculation that the membrane biogenesis in the region of ER transiting into ONM may be defining the outer and inner nuclear biomembrane. In the previous study that demonstrated the phosphatidyli ...
... (PIP1s) in Golgi [16,17]. These incongruities in the composition of the membrane continuum brought us to speculation that the membrane biogenesis in the region of ER transiting into ONM may be defining the outer and inner nuclear biomembrane. In the previous study that demonstrated the phosphatidyli ...
Isolation and Chemical Characterization of Plasma Membranes from
... Bulk preparation. To scale up the preparation procedure, the following modifications were made. For the initial fractionation of the 5000g pellet, it was resuspended in 5 ml of 50 % (w/v) sucrose made up as described previously and 40% sucrose layered on top. After centrifugation (2 h, 82 500g, in a ...
... Bulk preparation. To scale up the preparation procedure, the following modifications were made. For the initial fractionation of the 5000g pellet, it was resuspended in 5 ml of 50 % (w/v) sucrose made up as described previously and 40% sucrose layered on top. After centrifugation (2 h, 82 500g, in a ...
"VESICLE IN A BASKET" A Morphological Study of
... Examining the Os04 -fixed P6 fraction from surface to bottom, one finds in sections that it is composed of three layers : A layer, B layer, and C layer . The A layer (Fig. 1), occupying the surface of this fraction, appears to be the thickest . Fig . 2 is a high-power electron micrograph of the vesi ...
... Examining the Os04 -fixed P6 fraction from surface to bottom, one finds in sections that it is composed of three layers : A layer, B layer, and C layer . The A layer (Fig. 1), occupying the surface of this fraction, appears to be the thickest . Fig . 2 is a high-power electron micrograph of the vesi ...
Chloroplast structure: from chlorophyll granules to supra
... the answer is ‘no.’ While crystal structure studies of the protein complexes of photosynthetic membranes are continuing to make headlines, photosynthesis researchers are becoming increasingly aware of the fact that the crystal structures alone cannot explain many known functional properties of thyla ...
... the answer is ‘no.’ While crystal structure studies of the protein complexes of photosynthetic membranes are continuing to make headlines, photosynthesis researchers are becoming increasingly aware of the fact that the crystal structures alone cannot explain many known functional properties of thyla ...
Fractionation of Membranes from Metschnikowi`a
... since the cytosol of M . reukarrfii has a pH of 6-2(Aldermann & Hiifer, 1981). The idination of tyrosine residues of plasma-membrane proteins occurs at pH 7.5 in the presence of H 2 0 2and lactopcroxidase (Phillips & Morrison, 1970; 1971).The size of lactoperoxidase molecules (mol. wt 78000) prevent ...
... since the cytosol of M . reukarrfii has a pH of 6-2(Aldermann & Hiifer, 1981). The idination of tyrosine residues of plasma-membrane proteins occurs at pH 7.5 in the presence of H 2 0 2and lactopcroxidase (Phillips & Morrison, 1970; 1971).The size of lactoperoxidase molecules (mol. wt 78000) prevent ...
Cell Metabolism
... and could not be dissociated from the receptor under nondenaturing conditions (Wisely et al., 2002). Mutating conserved amino acids within the LBD that disrupt fatty acid binding significantly decreases HNF4 transcriptional activity, suggesting that this interaction is essential for receptor functio ...
... and could not be dissociated from the receptor under nondenaturing conditions (Wisely et al., 2002). Mutating conserved amino acids within the LBD that disrupt fatty acid binding significantly decreases HNF4 transcriptional activity, suggesting that this interaction is essential for receptor functio ...
SPA Enzyme Assay Design
... or generic. Another factor to consider when setting up the assay is that many enzymes require activation, either by post translational modification or the presence of co-factors, likely present in the cell lysate but for pure preparations it may be necessary to add any known co-factors. A wide range ...
... or generic. Another factor to consider when setting up the assay is that many enzymes require activation, either by post translational modification or the presence of co-factors, likely present in the cell lysate but for pure preparations it may be necessary to add any known co-factors. A wide range ...
Protein transport across the endoplasmic reticulum membrane
... conformational change, exposing a site on the ribosome to which a single Sec61 ⁄ SecY molecule could bind [25]. This is likely to be the molecule seen in recent EM structures of complexes of nontranslating ribosomes with either the SecY or the Sec61 complex [23,23a]. The bound SecY ⁄ Sec61 molecule ...
... conformational change, exposing a site on the ribosome to which a single Sec61 ⁄ SecY molecule could bind [25]. This is likely to be the molecule seen in recent EM structures of complexes of nontranslating ribosomes with either the SecY or the Sec61 complex [23,23a]. The bound SecY ⁄ Sec61 molecule ...
Leukocyte lipid bodies — Biogenesis and functions in inflammation
... electron microscopy [59]. The existence of membranous structures within lipid bodies of nonleukocytic cells could also explain how stanniocalcin and its membrane receptor are present at lipid bodies of ovarian steroidogenic cells and adipocytes [60]. Membranes within lipid bodies would account for t ...
... electron microscopy [59]. The existence of membranous structures within lipid bodies of nonleukocytic cells could also explain how stanniocalcin and its membrane receptor are present at lipid bodies of ovarian steroidogenic cells and adipocytes [60]. Membranes within lipid bodies would account for t ...
Dynamic balance between vesicle transport and
... experimental data from the literature to model in depth the various SCPs in each type and how they interacted. We developed analytical solutions that allowed us to calculate the relative relationships between different SCPs in a type that is needed to achieve overall balance between microtubule grow ...
... experimental data from the literature to model in depth the various SCPs in each type and how they interacted. We developed analytical solutions that allowed us to calculate the relative relationships between different SCPs in a type that is needed to achieve overall balance between microtubule grow ...
The Neuronal Membrane at Rest
... hydrophobic and will tend to associate readily with lipid. Regions with exposed polar R groups will be hydrophilic and will tend to avoid a lipid environment. Therefore, it is not difficult to imagine classes of rod-shaped proteins with polar groups exposed at either end, but with only hydrophobic g ...
... hydrophobic and will tend to associate readily with lipid. Regions with exposed polar R groups will be hydrophilic and will tend to avoid a lipid environment. Therefore, it is not difficult to imagine classes of rod-shaped proteins with polar groups exposed at either end, but with only hydrophobic g ...
Specialization of oleosins in OB dynamics during seed development
... allows precise determination of both lipid content and fatty acid composition. Recently, direct organelle mass spectrometry has been used to visualize lipid composition of cotton seed OBs (Horn et al., 2011). Nevertheless, in both cases, the methods are destructive. To observe lipid Downloaded from ...
... allows precise determination of both lipid content and fatty acid composition. Recently, direct organelle mass spectrometry has been used to visualize lipid composition of cotton seed OBs (Horn et al., 2011). Nevertheless, in both cases, the methods are destructive. To observe lipid Downloaded from ...
Could membrane lipids influence the receptor tyrosine kinase
... equally well with both EGFR monomers and EGFR dimers. The next question concerns the possible ways of binding between GM3 and EGFR receptor. The answer is probably hidden in the EGFR and GM3 structures. It is well-known that in the EGFR extracellular domains have from eleven to twelve potential glyc ...
... equally well with both EGFR monomers and EGFR dimers. The next question concerns the possible ways of binding between GM3 and EGFR receptor. The answer is probably hidden in the EGFR and GM3 structures. It is well-known that in the EGFR extracellular domains have from eleven to twelve potential glyc ...
Plasma membrane of Beta vulgaris storage root shows high water
... freshly prepared and plasma membrane vesicles were pre-incubated for 2 min in the presence of HgCl2 at a final concentration of 0.1 mM HgCl2. This concentration was maintained during the experiments. Regulation of water permeability To test regulatory mechanisms that shut down aquaporins present in ...
... freshly prepared and plasma membrane vesicles were pre-incubated for 2 min in the presence of HgCl2 at a final concentration of 0.1 mM HgCl2. This concentration was maintained during the experiments. Regulation of water permeability To test regulatory mechanisms that shut down aquaporins present in ...
Microscopy - BlackSage.com
... was probably first to combine two lenses into compound microscope (1595) ...
... was probably first to combine two lenses into compound microscope (1595) ...
Protein translocation pathways across the inner and outer
... outer membrane (Tom20 and Tom22); one half of this amphipathic helix having a hydrophobic surface, is recognized by the binding groove within Tom207, whereas the other half, which is positively-charged, is recognized by the Tom22 of TOM complex. The movement of preproteins through cytosol is associa ...
... outer membrane (Tom20 and Tom22); one half of this amphipathic helix having a hydrophobic surface, is recognized by the binding groove within Tom207, whereas the other half, which is positively-charged, is recognized by the Tom22 of TOM complex. The movement of preproteins through cytosol is associa ...
ref. #28 of the TIBS article
... constructs were expressed in vitro in the presence of dog pancreas microsomes, and MGD values were obtained by determining the number of residues between a given reference residue at the lumenal end of the TMH and the glycosylation acceptor Asn needed to get half-maximal glycosylation. Calibration: ...
... constructs were expressed in vitro in the presence of dog pancreas microsomes, and MGD values were obtained by determining the number of residues between a given reference residue at the lumenal end of the TMH and the glycosylation acceptor Asn needed to get half-maximal glycosylation. Calibration: ...
Structure and properties of the outer membranes of Brucella abortus
... detergent extraction, the ration porin/group 3 Omp is diminished in B. melitensis with respect to B. abortus, and a further reduction is observed in R mutants of the former species [50]. Association in a trimeric SDS-resistant state (a hallmark of porins) has been shown for group 2 (Omp2b in all lik ...
... detergent extraction, the ration porin/group 3 Omp is diminished in B. melitensis with respect to B. abortus, and a further reduction is observed in R mutants of the former species [50]. Association in a trimeric SDS-resistant state (a hallmark of porins) has been shown for group 2 (Omp2b in all lik ...
Synapses lecture 2012 De Camilli final
... If axon releases a large amount of neurotransmitter, it will also release neuropeptides that modulate the response of the post-synaptic cell. The neuropeptide prepares the post-synaptic cell to receive a barrage of neurotransmitter. ...
... If axon releases a large amount of neurotransmitter, it will also release neuropeptides that modulate the response of the post-synaptic cell. The neuropeptide prepares the post-synaptic cell to receive a barrage of neurotransmitter. ...
Model lipid bilayer
A model lipid bilayer is any bilayer assembled in vitro, as opposed to the bilayer of natural cell membranes or covering various sub-cellular structures like the nucleus. A model bilayer can be made with either synthetic or natural lipids. The simplest model systems contain only a single pure synthetic lipid. More physiologically relevant model bilayers can be made with mixtures of several synthetic or natural lipids.There are many different types of model bilayers, each having experimental advantages and disadvantages. The first system developed was the black lipid membrane or “painted” bilayer, which allows simple electrical characterization of bilayers but is short-lived and can be difficult to work with. Supported bilayers are anchored to a solid substrate, increasing stability and allowing the use of characterization tools not possible in bulk solution. These advantages come at the cost of unwanted substrate interactions which can denature membrane proteins.