Platelet Dense Granule Membranes Contain Both
... Reparation of densegmnules. Dense granules were prepared as described previouslypusing a modification of the method of Rendu et allRby centrifugation of a whole platelet homogenate through 40% metrizamide at 1lO.ooqS for 30 minutes. The sedimented pellet was markedly enriched in dense granules. The ...
... Reparation of densegmnules. Dense granules were prepared as described previouslypusing a modification of the method of Rendu et allRby centrifugation of a whole platelet homogenate through 40% metrizamide at 1lO.ooqS for 30 minutes. The sedimented pellet was markedly enriched in dense granules. The ...
isolation and characterization of the membrane
... The membrane envelope enclosing the bacteroids in soybean root nodules is shown by ultrastructural and biochemical studies to be derived from, and to retain the characteristics of, the host cell plasma membrane. During the early stages of the infection process, which occurs through an invagination, ...
... The membrane envelope enclosing the bacteroids in soybean root nodules is shown by ultrastructural and biochemical studies to be derived from, and to retain the characteristics of, the host cell plasma membrane. During the early stages of the infection process, which occurs through an invagination, ...
Microfluidic devices for drug discovery and analysis
... nanotechnology techniques, provides versatility in mixing fluids that can be controlled by external physical forces such as magnetic and electric fields. These microdevices may either be integrated into existing macrodevices, or constitute comprehensive analytical systems by themselves. The miniatur ...
... nanotechnology techniques, provides versatility in mixing fluids that can be controlled by external physical forces such as magnetic and electric fields. These microdevices may either be integrated into existing macrodevices, or constitute comprehensive analytical systems by themselves. The miniatur ...
MICROSCOPIC AND FUNCTIONAL STUDIES ON
... microscopy (TEM) was used to image extracellular vesicles from different sources. Because of their small size, 30-500 nm, examination of extracellular vesicles requires high resolution from research equipment. The results obtained in this study show that transmission electron microscopy and confocal ...
... microscopy (TEM) was used to image extracellular vesicles from different sources. Because of their small size, 30-500 nm, examination of extracellular vesicles requires high resolution from research equipment. The results obtained in this study show that transmission electron microscopy and confocal ...
The Ultrastructure of Megakaryocytes and Blood
... Zonation. The marginal zone varied in coalesce to form a long, paired membranwidth and usually lacked organelles and ous profile (figs. 3 and 5 ) , which is the demarcation membranes. The cytoplasm demarcation membrane (Yamada, '57). A contained a few ribosomes and vesicles and pair of membranes the ...
... Zonation. The marginal zone varied in coalesce to form a long, paired membranwidth and usually lacked organelles and ous profile (figs. 3 and 5 ) , which is the demarcation membranes. The cytoplasm demarcation membrane (Yamada, '57). A contained a few ribosomes and vesicles and pair of membranes the ...
Proteomics of Plasma Membranes from Poplar Trees Reveals
... Proteomics of Plasma Membranes from Poplar Trees Reveals Tissue Distribution of Transporters, Receptors, and Proteins in Cell ...
... Proteomics of Plasma Membranes from Poplar Trees Reveals Tissue Distribution of Transporters, Receptors, and Proteins in Cell ...
Title Nanofiltration and reverse osmosis surface topographical
... The use of surface roughness as a parameter is usually quantified as the average roughness and root mean squared roughness. However, quantifying membrane topography in the presence of surface topographical heterogeneities (redefined as surface defects throughout this study) can be challenging [21], ...
... The use of surface roughness as a parameter is usually quantified as the average roughness and root mean squared roughness. However, quantifying membrane topography in the presence of surface topographical heterogeneities (redefined as surface defects throughout this study) can be challenging [21], ...
Detection, Characterization, and Bioavailability of Membrane
... monitored with correction for development of any non-TBARS chromogen as previously described ( 15). Immediately before these assays were performed, we added butylated hydroxytoluene and deferoxamine (0.1 mM each) to preclude additional lipid decomposition during the assay itself. These measurements ...
... monitored with correction for development of any non-TBARS chromogen as previously described ( 15). Immediately before these assays were performed, we added butylated hydroxytoluene and deferoxamine (0.1 mM each) to preclude additional lipid decomposition during the assay itself. These measurements ...
Methods_Mol._Biol._591_185-199
... Since the discovery of the diffraction barrier in the late nineteenth century, it has been commonly accepted that with far-field optical microscopy it is not possible to resolve structural details considerably finer than half the wavelength of light. The emergence of STED microscopy showed that, at ...
... Since the discovery of the diffraction barrier in the late nineteenth century, it has been commonly accepted that with far-field optical microscopy it is not possible to resolve structural details considerably finer than half the wavelength of light. The emergence of STED microscopy showed that, at ...
Protection by chlorpromazine, albumin and bivalent cations against
... addition of the cells and the peptides into the cuvette. In other words, after dilution and equilibration in the cuvette, RBC became several times more sensitive to lytic action of the same amount of peptide. The magnitude of this effect is larger using bee venom and melittin as compared with P14A, ...
... addition of the cells and the peptides into the cuvette. In other words, after dilution and equilibration in the cuvette, RBC became several times more sensitive to lytic action of the same amount of peptide. The magnitude of this effect is larger using bee venom and melittin as compared with P14A, ...
Effects of Single Amino Acid Substitution on the Biophysical
... are considered as more difficult to deal with in clinical practice [1,2]. As a category of promising molecules, antimicrobial peptides (AMPs) have been widely investigated for their outstanding properties, such as rapid action, broad killing spectrum and difficulty in evoking resistance. Naturally e ...
... are considered as more difficult to deal with in clinical practice [1,2]. As a category of promising molecules, antimicrobial peptides (AMPs) have been widely investigated for their outstanding properties, such as rapid action, broad killing spectrum and difficulty in evoking resistance. Naturally e ...
Endocytosis Via Caveolae
... unusual topology in that the cytosolic N- and C-terminal domains are cytosolic connected by a hydrophobic sequence that is buried in the membrane but does not span the bilayer (23,24). Caveolins are palmitoylated in the C-terminal segment (25), they can be phosphorylated on tyrosine residues (26), t ...
... unusual topology in that the cytosolic N- and C-terminal domains are cytosolic connected by a hydrophobic sequence that is buried in the membrane but does not span the bilayer (23,24). Caveolins are palmitoylated in the C-terminal segment (25), they can be phosphorylated on tyrosine residues (26), t ...
Vesicle trafficking dynamics and visualization of zones of exocytosis
... is given by rairy¼1.22 k0/2 NA. For the microscope system used in this work operated in normal bright field mode, the limit of resolution that can be achieved according to the Rayleigh criterion is 203 nm. However, during operation of the microscope configured for high-resolution DIC, the resolution ...
... is given by rairy¼1.22 k0/2 NA. For the microscope system used in this work operated in normal bright field mode, the limit of resolution that can be achieved according to the Rayleigh criterion is 203 nm. However, during operation of the microscope configured for high-resolution DIC, the resolution ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessary for these pumpkin storage proteins to pass through the G ...
... are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessary for these pumpkin storage proteins to pass through the G ...
Intermediary Metabolism and Life-History Trade
... flightless morph has reduced wings and vestigial flight muscles (denoted SW; Zera et al. 1997; Zera and Harshman 2001). During the first week of adulthood, the LW(f) morph biosynthesizes a much larger quantity of somatic triglyceride, the main flight fuel of Gryllus (Zera et al. 1999; Zera and Larse ...
... flightless morph has reduced wings and vestigial flight muscles (denoted SW; Zera et al. 1997; Zera and Harshman 2001). During the first week of adulthood, the LW(f) morph biosynthesizes a much larger quantity of somatic triglyceride, the main flight fuel of Gryllus (Zera et al. 1999; Zera and Larse ...
Sensory TRP Channel Interactions with Endogenous Lipids and
... C-fiber functions, which often leads to hyperalgesia. NGF and PGs are known to only sensitize sensory excitability, rather than directly eliciting neuronal firing. A proposed mechanism for these two mediators states that the increased abundance of TRPV1 channel proteins on surface membranes is due t ...
... C-fiber functions, which often leads to hyperalgesia. NGF and PGs are known to only sensitize sensory excitability, rather than directly eliciting neuronal firing. A proposed mechanism for these two mediators states that the increased abundance of TRPV1 channel proteins on surface membranes is due t ...
Molecular Affinity and Permeability of Different Molecular Weight
... 480000, 250000, and 120000 Da chitosan membranes, respectively. As shown in Figure 5, during the extended soaking period, the adsorptive capacity curve stayed flat after 1 h. Some polymers that have carboxyl groups in the molecule, such as poly(itaconic acid) chitosan, have strong affinity (30). The ...
... 480000, 250000, and 120000 Da chitosan membranes, respectively. As shown in Figure 5, during the extended soaking period, the adsorptive capacity curve stayed flat after 1 h. Some polymers that have carboxyl groups in the molecule, such as poly(itaconic acid) chitosan, have strong affinity (30). The ...
6 Movement of Molecules Across Cell Membranes
... The molecules of any substance, be it solid, liquid, or gas, are in a continuous state of movement or vibration, and the warmer a substance is, the faster its molecules move. The average speed of this “thermal motion” also depends upon the mass of the molecule. At body temperature, a molecule of wat ...
... The molecules of any substance, be it solid, liquid, or gas, are in a continuous state of movement or vibration, and the warmer a substance is, the faster its molecules move. The average speed of this “thermal motion” also depends upon the mass of the molecule. At body temperature, a molecule of wat ...
Chapter 10
... with radioactive amino acids in a procedure known as a “pulse-chase” experiment. The location of the radiolabeled proteins within the cell was then determined by autoradiography and electron microscopy, revealing the cellular sites involved in the events leading to protein secretion. After a brief e ...
... with radioactive amino acids in a procedure known as a “pulse-chase” experiment. The location of the radiolabeled proteins within the cell was then determined by autoradiography and electron microscopy, revealing the cellular sites involved in the events leading to protein secretion. After a brief e ...
DEVELOPMENT OF JACALIN CONJUGATED NANOSTRUCTURED LIPID CARRIERS FORMULA
... into the lipid phase of Suppocire® NA pre-emulsion at varying concentration of 0, 5, and 10% with the lipid phase admixture was maintained at 3% within all formulations (F1 to F3). In order to produce comparable NLCs particle size using 60% of surfactantcosurfactant admixture, the amount of Suppocir ...
... into the lipid phase of Suppocire® NA pre-emulsion at varying concentration of 0, 5, and 10% with the lipid phase admixture was maintained at 3% within all formulations (F1 to F3). In order to produce comparable NLCs particle size using 60% of surfactantcosurfactant admixture, the amount of Suppocir ...
The p101 subunit of PI3Kγ restores activation by Gβ mutants
... TLC plate (Whatman) with 35 ml of 2 N acetic acid and 65 ml of n-propyl alcohol as the mobile phase. Dried TLC plates were exposed to Fuji imaging plates, and autoradiographic signals were quantified with a Fujifilm FLA-5000 imaging system (Raytest). Differences in the lots of the phospholipids used ...
... TLC plate (Whatman) with 35 ml of 2 N acetic acid and 65 ml of n-propyl alcohol as the mobile phase. Dried TLC plates were exposed to Fuji imaging plates, and autoradiographic signals were quantified with a Fujifilm FLA-5000 imaging system (Raytest). Differences in the lots of the phospholipids used ...
fulltext - DiVA Portal
... amino acids and proteins. One of those important breakthroughs was the emergence of a liposome system that eventually evolved to the membranes of the cells. What probably started as a simple double layer of lipids, forming vesicles that allowed the physical separation of a small space from the exter ...
... amino acids and proteins. One of those important breakthroughs was the emergence of a liposome system that eventually evolved to the membranes of the cells. What probably started as a simple double layer of lipids, forming vesicles that allowed the physical separation of a small space from the exter ...
isolation and characterization of membranes from the cells of maize
... In the last few years, attention has been directed to the separation of the endomembranes from plant cells. Several fractionation systems have been developed, but most of them aim to separate only one component of the system from a total homogenate of cells (Powell & Brew, 1974; Hodges & Leonard, 19 ...
... In the last few years, attention has been directed to the separation of the endomembranes from plant cells. Several fractionation systems have been developed, but most of them aim to separate only one component of the system from a total homogenate of cells (Powell & Brew, 1974; Hodges & Leonard, 19 ...
Clathrinmediated transport: assembly required
... cage-like structures (Fig 1). Clathrin heavy chains can be sub-divided into five structurally and functionally distinct regions: an aminoterminal globular domain; a highly flexible linker region composed of short α-helices; a distal segment; a proximal segment and a carboxy-terminal domain, which me ...
... cage-like structures (Fig 1). Clathrin heavy chains can be sub-divided into five structurally and functionally distinct regions: an aminoterminal globular domain; a highly flexible linker region composed of short α-helices; a distal segment; a proximal segment and a carboxy-terminal domain, which me ...
Changes in Typical Organelles in Developing Cotyledons of Soybean
... stained by PAS, CBB and osmium and could also be distinguished from other structures by their spherical shape . Their number and size increased around 30 OAF. Until they are better understood, we are calling these organelles protein-lipidsugar ( PLS) bodies because they are stained by reagents which ...
... stained by PAS, CBB and osmium and could also be distinguished from other structures by their spherical shape . Their number and size increased around 30 OAF. Until they are better understood, we are calling these organelles protein-lipidsugar ( PLS) bodies because they are stained by reagents which ...
Model lipid bilayer
A model lipid bilayer is any bilayer assembled in vitro, as opposed to the bilayer of natural cell membranes or covering various sub-cellular structures like the nucleus. A model bilayer can be made with either synthetic or natural lipids. The simplest model systems contain only a single pure synthetic lipid. More physiologically relevant model bilayers can be made with mixtures of several synthetic or natural lipids.There are many different types of model bilayers, each having experimental advantages and disadvantages. The first system developed was the black lipid membrane or “painted” bilayer, which allows simple electrical characterization of bilayers but is short-lived and can be difficult to work with. Supported bilayers are anchored to a solid substrate, increasing stability and allowing the use of characterization tools not possible in bulk solution. These advantages come at the cost of unwanted substrate interactions which can denature membrane proteins.