Role of cryo-ET in membrane bioenergetics research
... Subtomogram averaging is a technique which can both identify known proteins and determine previously unknown protein structures within tomograms [14,16–18,40] (Figure 2E). Individual subvolumes containing the protein of interest are extracted from the tomogram, aligned with each other and averaged t ...
... Subtomogram averaging is a technique which can both identify known proteins and determine previously unknown protein structures within tomograms [14,16–18,40] (Figure 2E). Individual subvolumes containing the protein of interest are extracted from the tomogram, aligned with each other and averaged t ...
ref. #29 of the TIBS article
... obtained by determining the number of residues between a given reference residue at the end of the transmembrane helix (the first Gln after the poly-Leu stretch) and the glycosylation acceptor Asn needed to achieve half-maximal glycosylation (see Figure 2B). We have shown previously that the P2 doma ...
... obtained by determining the number of residues between a given reference residue at the end of the transmembrane helix (the first Gln after the poly-Leu stretch) and the glycosylation acceptor Asn needed to achieve half-maximal glycosylation (see Figure 2B). We have shown previously that the P2 doma ...
Surface plasmon resonance spectroscopy for characterisation of
... Surface plasmon resonance (SPR) spectroscopy is a rapidly developing technique for the study of ligand binding interactions with membrane proteins, which are the major molecular targets for validated drugs and for current and foreseeable drug discovery. SPR is label-free and capable of measuring rea ...
... Surface plasmon resonance (SPR) spectroscopy is a rapidly developing technique for the study of ligand binding interactions with membrane proteins, which are the major molecular targets for validated drugs and for current and foreseeable drug discovery. SPR is label-free and capable of measuring rea ...
5 | structure and function of plasma membranes
... of its mosaic nature, a very fine needle can easily penetrate a plasma membrane without causing it to burst, and the membrane will flow and self-seal when the needle is extracted. The mosaic characteristics of the membrane explain some but not all of its fluidity. There are two other factors that he ...
... of its mosaic nature, a very fine needle can easily penetrate a plasma membrane without causing it to burst, and the membrane will flow and self-seal when the needle is extracted. The mosaic characteristics of the membrane explain some but not all of its fluidity. There are two other factors that he ...
Dynamics of interactions of photosensitizers with lipoproteins and
... 3. Results and discussion 3.1. Interactions of photosensitizers with membranemodels and LDL In a first step, our aim was to fulfill the set of steady state and dynamic data available on the interactions of the photosensitizers with membranes and LDL, i.e. the major structures involved in the cellula ...
... 3. Results and discussion 3.1. Interactions of photosensitizers with membranemodels and LDL In a first step, our aim was to fulfill the set of steady state and dynamic data available on the interactions of the photosensitizers with membranes and LDL, i.e. the major structures involved in the cellula ...
Hundred-thousand light holes push nanoscopy to go parallel
... using stimulated emission. The application of photoswitchable dye for targeted modulation super-resolution was first raised in 2003 (Hell 2003) and then experimentally demonstrated in 2005 (Hofmann et al. 2005). Such application of photoswitchable dye due to their low saturation intensity has previo ...
... using stimulated emission. The application of photoswitchable dye for targeted modulation super-resolution was first raised in 2003 (Hell 2003) and then experimentally demonstrated in 2005 (Hofmann et al. 2005). Such application of photoswitchable dye due to their low saturation intensity has previo ...
The proteomics of plant cell membranes
... developing strategies to increase plants’ natural defences. ...
... developing strategies to increase plants’ natural defences. ...
Lysenin: A sphingomyelin specific pore
... was 10–12 nm and there are pore-like structures of 3–5 nm diameter inside the hexagonal units (Fig. 1). Recent planar lipid bilayer experiments indicate that lysenin forms a voltagedependent large conductance channel in a sphingomyelindependent manner [28,29]. The apparent molecular weight of lyseni ...
... was 10–12 nm and there are pore-like structures of 3–5 nm diameter inside the hexagonal units (Fig. 1). Recent planar lipid bilayer experiments indicate that lysenin forms a voltagedependent large conductance channel in a sphingomyelindependent manner [28,29]. The apparent molecular weight of lyseni ...
Lipid interaction of the C terminus and association of the
... progressive spasticity and weakness of the lower limbs. Yeast and plant cells do not possess ATLs, but similar GTPases—Sey1p in Saccharomyces cerevisiae and Root Hair Defective 3 (RHD3) in Arabidopsis thaliana—may have an analogous function (9). Mutations in the plant homolog RHD3 cause ER morpholog ...
... progressive spasticity and weakness of the lower limbs. Yeast and plant cells do not possess ATLs, but similar GTPases—Sey1p in Saccharomyces cerevisiae and Root Hair Defective 3 (RHD3) in Arabidopsis thaliana—may have an analogous function (9). Mutations in the plant homolog RHD3 cause ER morpholog ...
Evaluation of Different Solvent Types on the Extraction of
... Barreiro et al., 2013), high growth rate, short harvesting cycle which is generally less than ten days (Cheah et al., 2014) and in (Chen et al., 2013) and further in (Taher et al., 2014), possess high disease resistance ability, high metabolites content and high biomass density (Roy and Pal, 2014). ...
... Barreiro et al., 2013), high growth rate, short harvesting cycle which is generally less than ten days (Cheah et al., 2014) and in (Chen et al., 2013) and further in (Taher et al., 2014), possess high disease resistance ability, high metabolites content and high biomass density (Roy and Pal, 2014). ...
Modification of intracellular membrane structures for virus
... the best-studied member of the Hepacivirus genus. HCV infection is a major cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma, and affects 170 million people worldwide4. Two viruses that are closely related to HCV, GB virus (GBV) and bovine viral diarrhoea virus (BVDV), are oft ...
... the best-studied member of the Hepacivirus genus. HCV infection is a major cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma, and affects 170 million people worldwide4. Two viruses that are closely related to HCV, GB virus (GBV) and bovine viral diarrhoea virus (BVDV), are oft ...
chemistry of phospholipids in relation to biological membranes
... about the location of the constituents at the C1 and C2 fatty acid ester position, but usually the information obtained does not warrant a complete molecular description of the phospholipid (Figure 2). It is possible to unravel a phospholipid family by using chromatography on silicic acid impregnate ...
... about the location of the constituents at the C1 and C2 fatty acid ester position, but usually the information obtained does not warrant a complete molecular description of the phospholipid (Figure 2). It is possible to unravel a phospholipid family by using chromatography on silicic acid impregnate ...
Etude in vitro de la stéatose hépatique induite par la - HAL
... protein was produced in vitro in various cell types, using heterologous expression systems. These studies showed that the HCV core protein was cytoplasmic, and either associated with the ER membrane or on the surface of lipid droplets [41, 42]. Subsequent studies have confirmed these observations [4 ...
... protein was produced in vitro in various cell types, using heterologous expression systems. These studies showed that the HCV core protein was cytoplasmic, and either associated with the ER membrane or on the surface of lipid droplets [41, 42]. Subsequent studies have confirmed these observations [4 ...
Modeling the Mechanics of Cell Division: Influence of
... The cell division cycle is a central process in biology, the essential mechanism whereby cells grow and duplicate (Carlson, 2007). The mechanics of cell division is an essential part of the epigenetic program that supports cellular reproduction in all living organisms (Boal, 2012). The division prog ...
... The cell division cycle is a central process in biology, the essential mechanism whereby cells grow and duplicate (Carlson, 2007). The mechanics of cell division is an essential part of the epigenetic program that supports cellular reproduction in all living organisms (Boal, 2012). The division prog ...
Effect of membrane composition on temperature activation of TRPV1
... different stimuli (Figure 1), and ion channels are crucial part of the cell membrane [15]. Most animal cells usually have relatively lower intracellular sodium concentration, while the potassium ...
... different stimuli (Figure 1), and ion channels are crucial part of the cell membrane [15]. Most animal cells usually have relatively lower intracellular sodium concentration, while the potassium ...
Promega Notes 39: Stabilized TMB Substrate for Horseradish
... NaCl) containing 1% BSA and 0.05% Tween® 20 (blocking buffer). The blot was incubated with the primary antibody, monoclonal anti-beta-galactosidase (Promega) diluted 1:5000 in blocking buffer, for one hour and washed three times for 5 minutes each in TBS containing 0.1% BSA. Goat anti-mouse IgG HRP ...
... NaCl) containing 1% BSA and 0.05% Tween® 20 (blocking buffer). The blot was incubated with the primary antibody, monoclonal anti-beta-galactosidase (Promega) diluted 1:5000 in blocking buffer, for one hour and washed three times for 5 minutes each in TBS containing 0.1% BSA. Goat anti-mouse IgG HRP ...
Bacterial Classification
... Ocular X Objective equals Total A measure of the relative velocity of light passing through a substance ...
... Ocular X Objective equals Total A measure of the relative velocity of light passing through a substance ...
enterocolitica Yersinia Type III Secretion System of Properties of the
... and trifluoroacetic acid (TFA). One volume of cell culture was mixed with 1 volume of 10% TCA, and the mixture was incubated for 20 min on ice. Insoluble material was collected by centrifugation at 20,000 ⫻ g for 20 min at 4°C, washed twice with 2 volumes of ice-cold acetone, and dried. To increase ...
... and trifluoroacetic acid (TFA). One volume of cell culture was mixed with 1 volume of 10% TCA, and the mixture was incubated for 20 min on ice. Insoluble material was collected by centrifugation at 20,000 ⫻ g for 20 min at 4°C, washed twice with 2 volumes of ice-cold acetone, and dried. To increase ...
Force development by the contractile vacuole
... and the mineral oil, the CV rounded against this force during its rounding phase. It can, therefore, be supposed that the tension at the surface of the CV increases during the rounding phase. We previously proposed a hypothesis (Tominaga et al., 1998a; Tominaga et al., 1999) that an increase in the ...
... and the mineral oil, the CV rounded against this force during its rounding phase. It can, therefore, be supposed that the tension at the surface of the CV increases during the rounding phase. We previously proposed a hypothesis (Tominaga et al., 1998a; Tominaga et al., 1999) that an increase in the ...
Red Blood Cell Glycophorins
... constitute a group of red blood cell (RBC) transmemgene has yielded negative results. All the expressed glycophbrane proteins that, although perhaps not widely appreciorins are 0-glycosylated proteins with their amino terminal ated in clinical hematology, have been important players in domains exter ...
... constitute a group of red blood cell (RBC) transmemgene has yielded negative results. All the expressed glycophbrane proteins that, although perhaps not widely appreciorins are 0-glycosylated proteins with their amino terminal ated in clinical hematology, have been important players in domains exter ...
Red blood cell glycophorins
... constitute a group of red blood cell (RBC) transmemgene has yielded negative results. All the expressed glycophbrane proteins that, although perhaps not widely appreciorins are 0-glycosylated proteins with their amino terminal ated in clinical hematology, have been important players in domains exter ...
... constitute a group of red blood cell (RBC) transmemgene has yielded negative results. All the expressed glycophbrane proteins that, although perhaps not widely appreciorins are 0-glycosylated proteins with their amino terminal ated in clinical hematology, have been important players in domains exter ...
Hepatitis C Virus - Arteriosclerosis, Thrombosis, and Vascular Biology
... HCV-infected Huh7 cells, the majority of core proteins are localized at the surface of cytosolic lipid droplets that are in contact with ER membranes containing the HCV replication complex,34 which is also the site for VLDL assembly.12 Thus, HCV may replicate at an ER domain enriched in neutral li ...
... HCV-infected Huh7 cells, the majority of core proteins are localized at the surface of cytosolic lipid droplets that are in contact with ER membranes containing the HCV replication complex,34 which is also the site for VLDL assembly.12 Thus, HCV may replicate at an ER domain enriched in neutral li ...
Vesicular transport of newly synthesized opsin from the Golgi
... and printed at a final magnification of XI 8,000. The use of all visible cells rather than a selected midcellular sample yielded a random collection of cells sectioned in different axial planes. In order to estimate the relative degree of labeling of various sources within photoreceptor inner segmen ...
... and printed at a final magnification of XI 8,000. The use of all visible cells rather than a selected midcellular sample yielded a random collection of cells sectioned in different axial planes. In order to estimate the relative degree of labeling of various sources within photoreceptor inner segmen ...
Theoretical studies of Membrane Proteins
... Membrane proteins are a large and important class of proteins. They are responsible for several of the key functions in a living cell, e.g. transport of nutrients and ions, cell-cell signaling, and cell-cell adhesion. Despite their importance it has not been possible to study their structure and org ...
... Membrane proteins are a large and important class of proteins. They are responsible for several of the key functions in a living cell, e.g. transport of nutrients and ions, cell-cell signaling, and cell-cell adhesion. Despite their importance it has not been possible to study their structure and org ...
Architectural remodeling of the tonoplast during fluid
... a variety of membrane-impermeable probes, 2,15,16,25 we consistently observed their eventual deposition in the vacuole. For such mature cells to endocytose constitutively, a stable tonoplast surface area/volume ratio must be preserved by an unknown accompanying process of membrane turnover. Based on ...
... a variety of membrane-impermeable probes, 2,15,16,25 we consistently observed their eventual deposition in the vacuole. For such mature cells to endocytose constitutively, a stable tonoplast surface area/volume ratio must be preserved by an unknown accompanying process of membrane turnover. Based on ...
Model lipid bilayer
A model lipid bilayer is any bilayer assembled in vitro, as opposed to the bilayer of natural cell membranes or covering various sub-cellular structures like the nucleus. A model bilayer can be made with either synthetic or natural lipids. The simplest model systems contain only a single pure synthetic lipid. More physiologically relevant model bilayers can be made with mixtures of several synthetic or natural lipids.There are many different types of model bilayers, each having experimental advantages and disadvantages. The first system developed was the black lipid membrane or “painted” bilayer, which allows simple electrical characterization of bilayers but is short-lived and can be difficult to work with. Supported bilayers are anchored to a solid substrate, increasing stability and allowing the use of characterization tools not possible in bulk solution. These advantages come at the cost of unwanted substrate interactions which can denature membrane proteins.