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Cells CELL STRUCTURE AND FUNCTION • • • Pretest – take a test on the cell, organelles and functions!! ? WHAT IS A CELL? LAB – Have different cells underneath several microscopes, (plant, leaf, nerve cells, bacteria, paramecium) draw at least 3 of the cells, record a description of their features – shape and internal parts. Use your observations to write a definition of a “cell”, try to classify the cells they draw into 2 or more groups. Put each cell in a particular group. • • Grade Pretest Watch video “Secrets of a Cell” 26 min. Worksheet Plant cell A. CELL THEORY • 1. CELL – Collection of living matter enclosed by a barrier that separates the cell from its surroundings • 2. CELL THEORY – ALL LIVING THINGS ARE COMPOSED OF CELLS – CELLS ARE THE BASIC UNITS OF STRUCTURE AND FUNCTION IN LIVING THINGS – NEW CELLS ARE PRODUCED FROM EXISTING CELLS B. BASIC CELL STRUCTURE • 1. CELL MEMBRANE/PLASMA MEMBRANE – a thin, flexible barrier around the cell • 2. CELL WALL – a strong layer around the cell membrane • Cell Membrane and Cell Wall – support and protect cells while allowing them to interact with their surroundings • 3. NUCLEUS – a large structure that contains the cells genetic material and controls the cells activities • 4. CYTOPLASM – material inside the cell membrane, but not including the nucleus C. PROKARYOTES AND EUKARYOTES • BIOLOGISTS DIVIDE CELLS INTO 2 CATEGORIES: EUKARYOTES AND PROKARYOTES. THE CELLS OF EUKARYOTES HAVE A NUCLEUS, BUT THE CELLS OR PROKARYOTES DO NOT. PROKARYOTES – have cell membranes and cytoplasm but do not contain nuclei. • – Example – bacteria, escherichia coli – live in intestines • • They grow, reproduce and respond to stimuli EUKARYOTES – do contain nuclei, cell membrane, cytoplasm and organelles – ORGANELLES - “little organs” specialized structures that perform important cellular functions • Example – some singular, most multicellular, all plants, animals and fungi Prokaryotic cell PLANT CELL Questions – WHAT IS THE MAIN DIFFERENCE BETWEEN PROKARYOTIC CELLS AND EUKARYOTIC CELLS » EUKARYOTIC CELS CONTAIN A NUCLEUS WHILE PROKARYOTIC CELLS DON’G – DO BACTERIAL CELLS CONTAIN A NUCLEUS? » NO. ALL BACTERIA ARE PROKARYOTES – WHAT ELSE DO EUKARYOTIC CELLS CONTAIN THAT PROKARYOTIC CELLS DON’T? » EUKARYOTIC CELLS CONTAIN ORGANELLES, WHICH ARE SPECIALIZED STRUCTURES THAT PERFORM IMPORTANT CELLULAR FUNCTIONS ASSESMENT #1 • What three statements describe the cell theory? – All living things are composed of cells. Cells are the basic units of structure and function in living things. New cells are produced from existing cells • What is the main characteristic that distinguishes eukaryotes from prokaryotes? – The cells of eukaryotes have a nucleus, but the cells or prokaryotes do not. • Name two structures that all cells have. – All cells have a cell membrane and cytoplasm Cell Structures – Cell wall • • – The main function of the cell wall is to provide support and protection for the cell It allows water, oxygen, carbon dioxide, and other substances to pass through the cell wall Nucleus • The nucleus controls most cell processes and contains the hereditary information of DNA – Chromatin and Chromosomes » Chromatin – the granular material visible within the nucleus » Chromosomes – chromatin condenses into chromosomes when the cell divides – threadlike structures containing genetic information that is passed from one generation of cell to the next Nucleus cont. • Nucleolus – A small dense region within most nuclei in which the assembly of ribosomes begins • Nuclear Envelope – A double membrane layer, which surrounds the nucleus. It is dotted with thousands of nuclear pores which allows material to move into and out of the nucleus. Cytoskeleton • The cytoskeleton is a network of protein filaments that helps the cell to maintain its shape. The cytoskeleton is also involved in many forms of cell movement. – microtubules – hollow tubes of protein about 25 nanometers in diameter. They maintain cell shape and can also serve as “tracks” along which organelles are moved – microfilaments – long, thin fibers that function in the movement and support of the cell. (tough flexible framework that supports the cell) Organelles in the cytoplasm – Ribosomes – small particle in the cell in which proteins are assembled: made of RNA and protein – Endoplasmic Reticulum – (ER) found in eukaryotic cells, which components of the cell membrane, and some proteins are assembled – rough ER – synthesis of proteins ribosomes stud the surface – smooth ER – contains a collection of enzymes that perform specialized tasks – synthesizing lipids ENDOPLASMIC RETICULUM The endoplasmic reticulum is the organelle in which components of the cell membrane are assembled and some proteins are modified. The endoplasmic reticulum illustrated is called rough endoplasmic reticulum because of the ribosomes on its surface. • What are ribosomes composed of? – RNA and Proteins • Where are ribosomes produced? – In the nucleolus • What do ribosomes produce – Proteins • What happens to these proteins after they are composed by ribosomes? – They move directly into the ER, where they are chemically modified • How is the smooth ER different from the rough ER? – Smooth surface • What is the function of the smooth ER? – Collection of enzymes that perform specialized tasks, such as synthesis of lipids. ORGANELLES CONT. • • • • • Golgi apparatus – enzymes in the Golgi apparatus attach carbohydrates and lipids to proteins – go to outside of cell Lysosome – small organelles filled with enzymes. Break down lipids, carbohydrates and proteins from foods into particles used by rest of cell Vacuoles – sac like structures that store materials such as water, salt, proteins and carbohydrates Chloroplast – only found in plants and some other organisms (not in animals) use the energy from sunlight to make energy food molecules in a process known as photosynthesis. Mitochondria – release energy from stored food molecules. Convert food into high energy compounds that the cell can use to power growth, development and movement. GOLGI APPARATUS Enzymes in the Golgi apparatus attach carbohydrates and lipids to proteins. Notice the stacklike membranes that make up the Golgi apparatus. Assesment #2 • Describe the functions of the endoplasmic reticulum Golgi apparatus, chloroplast and mitochondrion. – Cell membrane components and some proteins are assembled in the ER. Enzymes in the Golgi apparatus attach carbohydrates and liplids to proteins. Chloroplasts use sunlight to make food molecules. Mitochondria convert food molecules into highenergy compounds. • What does the cell wall provide for a cell? – The main function of the cell wall is to provide support and protection for the cell. • Describe the role of the nucleus in the cell. – The nucleus controls most cell processes and contains DNA • What are two functions of the cytoskeleton? – The cytoskeleton is a network of protein filaments that helps the cell maintain its shape. The cytoskeleton is also involvedin many forms of cell movement. Movement through the membrane Cell membrane • The cell membrane regulates what enters and leaves the cell and also provides protection and support – • Lipid bilayer – double layered sheet that forms the core of nearly all cell membranes – – intakes food and water, eliminates waste cell membranes contain protein molecules, carbohydrates are attached to proteins » figure 7-15 Diffusion – molecules move from an area of high concentration to low concentration • • Diffusion causes many substances to move across a cell membrane without the cells using energy Concentration – the mass of solute in a given volume of solution, or mass/volume (video from website) – Osmosis – allows water molecules to pass easily through most biological membranes • Osmosis is diffusion of water through a selectively permeable membrane – • does not require energy Selective Premeable – some substances can pass across them and others can not. – – – – isotonic – same strength hypertonic – above strength hypotonic – below strength figure 7-18 chart (short video, osmosis,from website) • Osmotic pressure – osmosis exerts a pressure known as osmotic pressure on the hypertonic side of a selectively permeable membrane. – example – osmotic pressure should produce a net movement of water into a typical cell that is surrounded by fresh water. If this happens, the volume of a cell will increase until the cell becomes swollen, and bursts. – ? How do cell walls protect cells from osmotic pressure? » Cell walls prevent the cells from expanding even under tremendous osmotic pressure Faciliated Diffusion – movement of specific molecules across cell membranes through protein channels Example – some only allow glucose to pass through the channel does not require energy – Active transport – material moves from an area of lower concentration to an area of greater concentration (video on website) • Requires input of energy – Endocytosis – process by which a cell moves large amounts of material across the cell membrane by turning part of the cell membrane inside out. » Phagocytosis – large particles taken into the cell » Exocytosis – large particles taken out of the cell » Video on exo and endo on website • Passive transport – video Assesment #3 • What processes are made possible by the cell membrane? – The cell membrane regulates what enters and leaves the cell • How does diffusion affect cells? – Diffusion causes many substances to move across a cell membrane without the cell using energy • Describe how water moves during osmosis. – Water tends to diffuse from a region where it is highly concentrated to one where it is less concentrated. Water will move across a membrane until equilibrium is reached. • What is the basic structure of a cell membrane? – The core of nearly all cell membranes is a double layered sheet called a lipid bilayer. Most cell membranes contain protein molecules that run through the lipid bilayer, and carbohydrate molecules are attached to the membrane that scientists call the membrane a mosaic of molecules • How does active transport differ from diffusion? – Active transport requires the input of energy , while diffusion does not require energy