Download DNA and Mitosis - Birmingham City Schools

AGENDA
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•
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BELL RINGER
NOTES
CLASSWORK
Homework:
Complete Classwork if necessary
Essential Question:
What is the structure of DNA,
and how does it function in
genetic inheritance?
GOAL(S) –
I will be able to identify the structural
components within a model of DNA including
monomer units and hydrogen bonds.
• Cite and evaluate evidence that supports
Watson and Crick's model of the double helix
structure of DNA.
•
BELL RINGER
1.
2.
What is DNA?
List anything you know about DNA (from
readings, class, TV…?)
What is DNA?




DNA is the genetic material found in cells
Stands for: “Deoxyribonucleic Acid”
Is made up of repeating nucleic acids
It’s the “Unit of Heredity”

DNA is found in the cytoplasm of prokaryotes
and the nucleus of eukaryotes

The nucleus of a human cell contains 30,000 or
more genes in the form of DNA called a genome

Purpose: DNA controls the production of
proteins in the cell
 This is essential to life!
 DNA  RNA  Proteins

DNA is packaged tightly into
pieces called chromosomes that
are visible during cell division

Each chromosome includes
several thousand genes

Each gene contains the directions
to make one or more proteins
▪ Proteins are made of amino acids

These proteins play a key role in
the way we look and grow…ever
hear someone say “it’s in your
genes?”
One
Chromosome
Contains many genes
Each gene codes for a protein
Ex. Keratin protein

Specialization
 In embryo, all genes on the
DNA is “on”. This
undifferentiated cell (stem
cell) can develop into any
type of cell.
 Specialization occurs when
certain genes are turned
“off” and other genes
remain “on” – making a
particular type of cell
▪ Ex. Muscle cells and Nerve
cells in your body have the
same DNA, but they have
different genes activated.
Think of DNA as a spiral staircase!

DNA is comprised of two
strands that twist around
each other, called a double
helix
 Discovered by Watson and
Crick in 1953

“Twisted ladder structure”


DNA is a made of
building blocks called
nucleotides
A nucleotide is made of:
 one phosphate
 one 5-carbon sugar
(called deoxyribose)
 one nitrogen base
▪
▪
▪
▪
Adenine
Thymine
Guanine
Cytosine

Nucleotides put together make up the DNA strand!

The sides, or
“backbone” of the
DNA are composed of
alternating
phosphate-sugar
groups


Each “rung of the ladder”
is made up of
complementary
nitrogenous base pairs
The four bases are A
(adenine), T (thymine), G
(guanine), and C (cytosine)
 A pairs with T (2 H Bonds)
 G pairs with C (3 H Bonds)
DNA Source
Adenine Thymine Guanine Cytosine
Calf Thymus
1.7
1.6
1.2
1.0
Beef Spleen
1.6
1.5
1.3
1.0
Yeast
1.8
1.9
1.0
1.0
Tubercle Bacillus
1.1
1.0
2.6
2.4

They form weak hydrogen bonds that hold
the DNA strand together and are the reason
DNA can be replicated
 A::T forms 2 H-bonds, and C:::G forms 3 H-Bonds
AGENDA
• Bell Ringer
• Notes
• Graphic
Organizer/Classwork
GOAL
• Identify and describe the function of
molecules required for replication and
differentiate between replication on
the leading and lagging DNA strands.
HOMEWORK
• Complete classwork if
needed
Bell Ringer
1.
DNA is packaged into pieces. What are
these pieces called?
2.
There are thousands of genes on a
chromosome. A single gene contains the
directions to make what?
3.
The base adenine (A) always pairs with
____________, while the base guanine (G)
always pairs with _________________.
Making a new strand

DNA replication is the process of
producing 2 identical replicas from one
original DNA molecule
 Replicate means “to copy”

During replication, the DNA molecule
separates into two strands, and builds two
new complimentary strands using the
base pairing rules (A::T, C:::G)

The molecule is unwound and “unzipped”
with the help of helicase, an enzyme!

Step 1: DNA unwinds, then
“unzips,” exposing the N-bases
(remember, the bases are ATCG)

Step 2: New DNA N-bases are
added to each side of the
molecule, making two separate
strands
 If the unzipped side read ATCG,
then TAGC would be added to that
side. Now it is an independent
strand!
http://www.youtube.com/watch?v=hfZ8o9D1tus

Each new DNA strand (daughter chromosome)
is made up of 1 strand from the original DNA
(blue) and one new strand (red)

Given one strand, you can always find the other
strand using base pairing rules!

Let’s practice!
If the DNA sequence of bases on one strand was
G C T A C A T, what would the complementary
side be during replication?

CGATGTA
Lets look at the enzymes involved in DNA Replication

Semi-conservative =
one of the parent DNA
strands is passed to the
daugher DNA + one
new strand
SNEAK PREVIEW:
DNA REPLICATION
PLAYERS (enzyme
review)

The enzyme unwinds the chain, breaking the Hbonds between the complementary base pairs (A-T,
G-C).
Helicase

DNA-RNA-Protein (see ani)
YOU TUBE DNA replication (1:05)
• also called DNA gyrase
• Helps to unwind double helix by
easing tension caused by the
untwisting action of the helicase
Enzyme
DNA
Enzyme


Topoisomerase Youtube I and II (1:45)
Topoisomerase Animation (2:16)



Newly formed single strands tend to want to
reform the hydrogen bonds that have been
broken
These proteins help to stabilize the DNA
strands as they are being replicated
By preventing rejoining of DNA strands
Also known as
Primase
Helicase
= the enzyme
that makes RNA
nucleotides
into a primer
 Nucleotides for the starting point
for DNA replication
 Short strands of RNA


Elongates the strand by adding DNA
nucleotides on leading strand
Also proofreads and corrects the DNA
strand
These are much like the typical DNA nucleotides,
except that instead of having one phosphate they
have three.
 This gives them similar energy storage capabilities
as ATP
 DNA Pol III requires energy to synthesize DNA and
it gets when two phosphates are removed from
these nucleosides

7.LEADING STRAND
8. LAGGING STRAND


Template strand of DNA
Continuous addition of
nitrogenous bases
in 5’ to 3’ direction

McGraw-Hill Replication Fork

Other DNA strand
Forms short strands of
Okazaki fragments (that
will be joined later)
in the 5’ to 3’ direction

DNA Replication You Tube (1:35)

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The short strands of newly made DNA
fragments on the lagging strand are called
Okazaki fragments after the Japanese
Biochemist Reiji Okazaki.


Cuts off RNA primers and fills in with DNA
(between Okazaki fragments) –lagging
strand
Can proofread
a linking enzyme joins the strands
Example: joining two Okazaki
fragments together.
DNA ligase
5’
3’
Okazaki Fragment 1
Lagging Strand
Okazaki Fragment 2
3’
5’

Sometimes DNA Pol III inserts the wrong
nucleotide.
 Even though the average error rate is one in every
million base pairs, there can still be harmful
consequences if these errors are not repaired.

Repair includes the use of DNA Pol II and
DNA Pol I which have exonuclease functions
that allow them to remove mismatched DNA
nucleotides and replace them with the
correct ones.
Called Replication Bubbles
 They will eventually all meet to form whole
replicated strand

EM of DNA replication
Origins of Replication
• sites along the DNA molecule where
enzymes start the DNA replication - then
proceeds in both directions to form
“bubbles”
Replication Forks
Y-shaped regions of replicating DNA
molecules where new strands are
growing.

Anti-parallel strand builds in the opposite
direction (but always in 5’ to 3’ direction)


Summary Youtube of DNA replication (4:11)
Good explanation of the 5’ to 3’ strands and
leading and lagging strands

https://www.youtube.com/watch?v=z685FFq
mrpo
1.
What are the steps in
DNA replication?
1.
What is the outcome of DNA
replication?
1.
Given the following strand of
DNA, what would the
complementary side read?
• I will be able to create
a graphic illustrating the
amount of time a cell spends in each phase of
the cell cycle.
• I will be able to identify cells in each phase if
given an image of the cell.
• I will be able to communicate information about
the relationship between the cell cycle and the
growth and maintenance of an organism.
• I will be able to illustrate chromosome behavior
during mitosis using chromosome models.
• I will be able to distinguish between replicated
and un-replicated chromosomes.
• I will be able to demonstrate the events and
cellular processes involved in each stage of
mitosis.
• I will be able to relate errors in cell cycle control
mechanisms to uncontrolled cell growth (cancer

C T G A A T C G A
How does a cell grow and divide?
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
The Cell Cycle describes the life of a cell from
birth to death
There are three main parts of the cycle:
 Interphase-Normal cell activities; broken up into 3
parts
 Mitosis-The process of cell division (1 cell
becomes 2!)
 Cytokinesis-The division of the organelles and
cytoplasm following mitosis
Interphase is indicated in grey-it is the longest phase of the cycle,
broken into 3 parts
 Mitosis is indicated in pink-we will discuss the stages of mitosis later!


G1 phase (Gap/Growth 1)-Period of
cell growth
 Cells can remain in the G1 phase
indefinitely
 Called G0

S phase (Synthesis)-Period when
DNA replication occurs
 Once a cell copies its DNA, it must
divide
 S phase allows daughter cells to have
exact copy of parent DNA after
division!

G2 phase (Gap/Growth 2)-Cell
growth and preparation for Mitosis

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Mitosis is a form of asexual reproductionmeans only 1 organism required
Occurs in response to the body’s need for
growth and repair
4 stages of mitosis: Prophase, Metaphase,
Anaphase, Telophase
 We’ll talk more about this in a bit!

The cell cycle ends with cytokinesis the
division of the cytoplasm
 Accompanies mitosis

This means one cell has divided into two cells,
and those two cells can continue with their
own independent cell cycles!

http://highered.mheducation.com/sites/0072
495855/student_view0/chapter2/animation__
how_the_cell_cycle_works.html

Cyclins-Proteins that regulate the rate of the
cycle
 Internal regulation-cell cycle can’t proceed until
certain levels of these proteins are reached (ex.
Poor nutrition cell stays in G1)
 External regulation-cycle can speed up or slow
down
▪ Do you think a paper cut on your finger would cause the
cell cycle to speed up or slow down?

Sometimes errors in the
cell cycle can lead to
cancer Errors can be genetic or due
to an environmental toxin

Internal regulation error
followed by external; cells
cannot “feel” their
neighbors, and thus begin
uncontrolled division
 Lack density dependence
(tumor) and anchorage
dependence (metastasized
cancer cells)

From 1: 15
https://www.youtube.com/watch?v=IeUANxF
VXKc
1.
2.
3.
Label the diagram on the
right with the appropriate Cell divides
stage of the cell cycle.
Why would the cycle
need to pause at
Growth and
checkpoints before
preparation for
division
moving to the next
stage?
Explain how cancer is
related to regulation of
the cell cycle.
Cytoplasm
divides: 1
cell is now 2
Cell Growth: Cell may
stay indefinitely if it
does not meet
checkpoints
DNA is replicated.
Stages of Asexual Cell Division

Recall that the cell cycle is made up of three
main parts
 Interphase (G1, S, and G2)
 Mitosis
 Cytokinesis

Mitosis refers to the division of the cell
 Asexual reproduction for unicellular eukaryotes
 Occurs in response to the bodies need for growth
and repair



Occurs in eukaryotes
1 cell divides to produce 2 daughter cells
These cells are identical to the original cell
same number of chromosomes!

What happens when the cell leaves
interphase and is ready to begin division…?

What Happens?
 Nuclear membrane dissolves
 Chromatin condenses into
chromosomes
▪ Chromatin: uncondensed DNA
(looks like spaghetti)
▪ Chromosome: condensed DNA
(looks like X’s)
 Centrioles move to opposite
ends of the cell
 Spindle forms and spindle
fibers extend from one side to
the other

What Happens?
 Centromeres (middle of
chromosome) attach to
spindle fibers
 Chromosomes are pulled
to the middle of the cell

What Happens?
 Spindle fibers pull
chromosomes apart
 Each sister chromatid
moves toward
opposite end of the
cell

What Happens?
 Nuclear membrane
reforms
 Spindle fibers
disappear
 Animal Cells:
▪ Cell membrane pinches
 Plant Cells:
▪ New cell wall begins to
form

What happens?
 Division of the cytoplasm and organelles
 1 cell is now 2 identical cells!
INTERPHASE
ANAPHASE
PROPHASE
METAPHASE
TELOPHASE
CYTOKINESIS