Download Transduction of DNA information through water and electromagnetic

Transduction of DNA information through water and electromagnetic waves
Luc Montagniera,b, Emilio Del Giudicec,∗ , Jamal Aı̈ssab , Claude Lavalleea , Steven Motschwillerd ,
Antonio Capolupoe,f , Albino Polcarig , Paola Romanog,h , Alberto Tedeschii , and Giuseppe Vitielloe,f
a
World Foundation for AIDS research and Prevention (UNESCO), Paris, France
Nanectis Biotechnologies, S.A. 98 rue Albert Calmette, F78350 Jouy-en-Josas, France
Sezione INFN, I-20122 Milano, Italy (retired) and Centro Studi Eva Reich, Via Colletta, 55, I-20122 Milano, Italy
d
Chronix Biomedical, GmbH, Goetheallee, 8, 37073 Göttingen, Germany
e
Dipartimento di Fisica E.R.Caianiello Universitá di Salerno, Fisciano (SA) - 84084, Italy
f
INFN Gruppo collegato di Salerno, Fisciano (SA) - 84084, Italy
g
Dipartimento di Scienze e Tecnologie, Universitá del Sannio, Benevento - 82100, Italy
h
SPIN-CNR, Universitá di Salerno, Fisciano (SA) - 84084, Italy and
i
WHITE Holographic Bioresonance, Via F. Petrarca, 16, I-20123 Milano, Italy
b
arXiv:1501.01620v1 [q-bio.OT] 27 Dec 2014
c
The experimental conditions by which electromagnetic signals (EMS) of low frequency can be
emitted by diluted aqueous solutions of some bacterial and viral DNAs are described. That the
recorded EMS and nanostructures induced in water carry the DNA information (sequence) is shown
by retrieval of that same DNA by classical PCR amplification using the TAQ polymerase, including
both primers and nucleotides. Moreover, such a transduction process has also been observed in
living human cells exposed to EMS irradiation. These experiments suggest that coherent long range
molecular interaction must be at work in water so to allow the observed features. The quantum
field theory analysis of the phenomenon is presented.
I.
INTRODUCTION
This paper is an overview of what we have achieved during the past ten years in this new field of Biology: the role
of water memory and electromagnetic waves in biological processes, including pathological conditions. The reported
data is not only of theoretical interest, but leads to many medical applications.
This work could not have been done and analyzed without the constant interaction of biologists and physicists.
The quantum field theoretical analysis of the phenomenon points to the crucial role played by coherent molecular
dynamics.
II.
ELECTROMAGNETIC SIGNALING OF DNA
A.
The detection of electromagnetic signals (EMS)
On 13 July 2005, (the eve of Bastille Day in France), by using a device previously designed by the Jacques Benveniste
team to detect electromagnetic signals in water dilutions of biologically active compounds, and with the help of one of
his former collaborators, Dr. Jamal Aı̈ssa, two of us (LM, JA) observed for the first time an increase of amplitude and
frequency of the recorded electric signals emitted by some high dilutions of filtrates of bacteria (Mycoplasma pirum,
then Escherichia coli). This was the beginning of an extensive investigation on the role and the molecular origin of
this new phenomenon (Montagnier, Aı̈ssa, Ferris, et al., 2009; Montagnier, Aı̈ssa, Lavallee, et al., 2009; Montagnier,
Aı̈ssa, Del Giudice, et al., 2011).
We soon discovered that DNA was the main source of the initiation of electromagnetic signals in water. In contrast
to the fresh preparation of biological fluids (blood plasma, culture media) which lose their capacity of inducing EMS
in water upon freezing, DNA extraction could be done from frozen material without losing its EMS capacity.
In fact, as we will see below, this property of some bacterial and viral DNA sequences of emitting EMS is like
an indelible tag, and is faithfully transmitted to water structures. The bacterial species with pathological potential
cultured in standard growth media yield DNA with EMS capacity. However, we noticed that one apathogenic strain
of E. coli used for DNA cloning lacks this capacity as does a probiotic bacterium (Lactobacillus).
The size of DNA fragments emitting EMS ranges between 104 base pair (LTR fragment of HIV) to several kilo-bases
∗
deceased 31 January 2014
2
Figure 1: Measuring EMS in water decimal dilutions of DNA. In yellow, dilutions emitting EMS; bottom: recording of EMS
in milliseconds
(adhesin gene of Mycoplasma pirum: 1.5 - 3 kbp). Some PCR (Polymerase Chain Reaction) amplicons1 , of 400 - 500
bp, have been found good emitters for the transduction experiments (see below) . For the capture of EMS in water
dilutions, the conditions are very strict, and are the same for the extracted DNA as for the fresh unfrozen samples of
plasma or of culture medium:
Double filtration: through 450 nm and then 100 nm Millipore filters, for detection of EMS having a bacterial origin;
or 450 nm then 20 nm (Whatman anotop) for EMS of viral origin (only tested for some small virus DNA and HIV
DNA). The usual starting concentration is 2 ng of DNA/1 ml diluted 100 times (10 mls) for filtration.
Dilutions: several decimal dilutions are made in conic plastic tubes (Eppendorf) usually 0.1 ml/0.9 ml of water under
a laminar flow hood. (Fig. 1) Strong vortex shaking (for 15 seconds) is made at each dilution at room temperature.
Water is purchased from commercial firms (usually 5 prime water, DNase - RNase free from Sigma). Usually, the
EMS-emitting dilutions are between 10−7 to 10−13 for bacteria, 10−6 to 10−10 for small viruses (as HIV1). The first
lower dilutions are apparently “silent”, not emitting detectable signals.
Capture of EMS: As the EMS are produced by resonance,(Montagnier, Aı̈ssa, Del Giudice, et al., 2011) we use either
an artificial excitation (7 Hz which was found to be the minimally active frequency) in a closed environment shielded
by mu-metal, or in open air exposed to the background ambient noise. Usually, this background noise predominates
in the 50 - 300 Hz range, so that the positive EMS, which are in the range of 500 - 3000 Hz, are easily detected.
In the measurement room, cell phones should be turned off (battery removed) as some phones are regulated by low
frequency signals.
B.
Evidence that EMS emission depends on specific modification of the DNA molecule
The diversity of the DNA sequences emitting EMS does not indicate any clue as to attribute this EMS emission
property to specific sequences.
However, we have studied an interesting situation in the case of HIV-infected patients: here, besides EMS produced
by HIV DNA (nanostuctures filtering at 20 nm pores), we detected EMS filtering at 100nm pores and retained at 20
nm pores produced by DNA of an intracellular bacterium present in red blood cells. Surprisingly, these ”bacterial”
EMS were found to be produced in part by human DNA sequences integrated in or strongly associated with the
bacterial DNA. The same DNA sequences belonging to the chromosomal genome of the same patient never produced
EMS.
Moreover, the same sequence was found present in the red blood cells of some healthy individuals, HIV negative;
but in these HIV negative individuals this sequence was found not to emit signals. This would indicate that the
modification of this DNA resulting in EMS emission occurred only under pathogenic conditions. This modification
was maintained in all molecules derived by PCR amplification (amplicon).
As EMS are so far only detected in patients suffering of various chronic diseases, it is tempting to speculate that
there is a common biochemical modification of the DNA of infectious bacteria and/or viruses present in such diseases.
This modification, which remains to be determined, should be different from a base change (mutation), since there is
1
An amplicon is a piece of DNA or RNA that is the source and/or product of natural or artificial amplification or replication events,
usually PCR.
3
DNA
Water
Naneons
EMS
EMS
Water
Naneons
PCR
DNA
7 Hz
2
Figure 2: Scheme of DNA
transmission through EMS and water nanostructures (naneons)
Water-mediated photonic transmission of DNA
DNA
Water
Naneons
EMS
Computer
Digitized
Receiver
EMS
Analog
PCR
Computer
DNA
Water
Naneons
Figure 3: Digital transmission of DNA EMS to a distant laboratory
no difference in the base sequence of the previously mentioned amplicons in diseased patients, compared to healthy
individuals.
C.
Water nanostructures and EMS do carry DNA information
Our formerly reported experiments (Fig. 2 and ref. (Montagnier, Aı̈ssa, Lavallee, et al., 2009)) indicate that the
ability of EMS production can be transmitted from tube 1 containing an emitter DNA dilution to tube 2 of “naive”
water, provided the system is excited overnight by electromagnetic waves of a minimal frequency of 7 Hz. Presumably
tube 1 transmits waves to the water in tube 2, which did not originally contain any trace of the DNA at the origin of
the signals.
The emission of EMS by the exposed tubes is thus a resonance phenomenon, dependent on external wave input.
More importantly, these EMS carry specific information of the initial DNA, as shown by retrieving the DNA by PCR
in the recipient tube.
This experiment has been repeated many times in our laboratory, with extraordinary precautions taken to avoid
contamination in the PCR step, and many controls were always done. Omission of any of the main parameters of the
procedure (7 Hz excitation, mu metal shield, time of exposure to the 7 Hz excitation, any ingredient of the PCR) as
well as any minor detail of the protocol will result in failure of the experiment.
To further make the demonstration unassailable, the EMS carrying the DNA information were recorded as a digital
file and sent via Internet to a recipient laboratory where work on this DNA or on the bacterium or virus which was
the source of that DNA had never been done (Fig. 3). Several labs in Italy and Germany accepted the challenge.
Here, as an example, we show the results obtained at Göttingen University using a recorded file (digitized in a lap
top computer in our laboratory) of ribosomal 16S DNA from Borrelia burgdorferi (Fig. 4).
In the German laboratory, the electric current resulting from the digital file communicated by our lab was converted
to analog and was amplified. The current was then connected to a solenoid. A tube of water was inserted in the
solenoid and in this way was submitted to the induced modulated magnetic field for one hour. Then the PCR
ingredients were introduced in an aliquot of water from the tube, and after 40 PCR cycles of amplification the original
DNA was detected, as shown by a specific band in gel electrophoresis of the expected molecular weight.
These intriguing results raised several questions :
4
Water-mediated photonic transmission of DNA
Gel electrophoresis of the PCR DNA product (Borrelia Burgdorferi)
E.Schutz et al. Goettingen, 2011
Figure 4: Gel electrophoresis of Borrelia 16S DNA amplified by PCR from a water tube having received the DNA specific EMS
(through computer, sound card and amplifier, not shown in this Figure)
1) How a DNA polymerase (the TAQ polymerase of a thermophilic bacterium) can “read” a genetic code on water
structures?
2) What about other DNA polymerases of procaryotic and eucaryotic cells? Do they have the same capacity?
Although still at its early stages, the theoretical study of how water structures can store molecular information
and transport it by electromagnetic waves gives a crucial role to the coherent molecular dynamics in the formation
of water nanostructures (see below and (Montagnier, Aı̈ssa, Del Giudice, et al., 2011)). We need, however, further
theoretical analysis for a complete understanding of the phenomenon, especially because we have recent evidence that
some other DNA polymerases have the same capacity as the TAQ polymerase to read water messages and can act in
living cells.
D.
Transduction of DNA in living cells
The modified DNA transduction system is shown in figure 5.
Instead of magnetizing water in a tube placed inside a solenoid reading the modulated current from the recorded
EMS signature, we placed inside the solenoid a flask containing cultured cells; the flask was placed in a vertical
position for cells growing in suspension, and in a horizontal position for cells adhering to the surface of the flask. The
voltage (between 2 - 4 volts) applied to the solenoid was adjusted in order to not generate heat damaging the cultured
cells. This weak intensity was compensated by the duration time of exposure, between 5 to 10 days. A control flask
was always placed outside the solenoid in the same 37 ◦ C incubator as the exposed flask.
We used several recorded EMS files, including the 16S Borrelia and the 194 bp HIV1 LTR amplicon all having been
previously shown to be good at transducing their DNA through water.
We tested several immortalized human cell lines derived from leukemias, or cancers: the HL60, originated from a
myeloblastic leukemia, the U937, derived from a lung lymphoma, the MCF7, derived from a breast adenocarcinoma.
In addition, we tested normal cells: the MRC5 diploid fibroblast cell line, derived from the lung of a human embryo,
T lymphocytes from a healthy blood donor activated with PHA, and interleukin 2.
Results were striking: All cells of tumor origin synthesized Borrelia 16S DNA after they were exposed for several
days to magnetic field modulated by the EMS of Borellia 16S DNA. At the same time, cell growth was inhibited,
ending in cell death. DNA was extracted from the dying cells and the Borrelia amplicon was detected by PCR.
Remarkably the resulting amplicon was found to be EMS emitter, showing that this initial property was not lost
during the complex transmission of DNA information. The normal differenciated MRC5 cells and the T lymphocytes
were not affected in their growth under the same culture conditions and the Borrelia amplicon could not be detected
in these cells. The 194 bp HIV LTR amplicon had no effect on the tumor cells.
These preliminary results indicate that the tumoral cell lines so far investigated do possess the enzymatic ability of
reading the water nanostructures carrying the DNA information. It remains to be determined whether or not normal
embryonic totipotent stem cells have the same ability to read the DNA sequence signals.
5
Figure 5: Scheme of transmission of Borrelia 16S DNA through EMS and naneons into U937 or HL60 cells
III.
THEORETICAL ANALYSIS
In the previous Section we have reported the experimental observation that EMS can be emitted by diluted aqueous
solutions of bacterial and viral DNA under proper conditions. Moreover, it has been observed that duplication of
the emitting DNA segment can be obtained by using pure water exposed to the corresponding DNA EMS and, upon
addition of enzymes, primers, etc., submitted to PCR cycles. Such a transduction process has been observed to
occur also in EMS exposed living cells of tumoral origin. These experimental observations suggest that long range
molecular interaction must be at work in water so to allow the observed properties. Indeed, since in the transduction
process the high level of sequential ordering among several hundreds of nucleotides entering the transduced DNA
chain is obtained, we are clearly in the presence of collective molecular dynamical behavior of water. In quantum field
theory (QFT) it is known that the ordering of the elementary components of a system is achieved as a result of the
spontaneous breakdown of symmetry and constitutes the observable manifestation of coherence (Blasone, Jizba and
Vitiello, 2011; Fröhlich, 1977; Umezawa, 1993; Vitiello, 1998;). Ordering is thus not the result of short range forces,
but of long range collective coherent correlation. The classical behavior of the ordered pattern derives from the fact
that in coherent states the ratio between the quantum fluctuation ∆n in the correlation modes and their condensate
number n is ∆n/n = 1/|α| and quantum fluctuations are thus negligible for high |α|, which denotes the coherent
strength. In the present case, the symmetry which gets broken is the rotational symmetry of the electrical dipoles
of the water molecules and correlation modes are the ones associated to the dipole waves (similar to spin waves in
ferromagnets) (Del Giudice, Doglia, Milani and Vitiello, 1985, 1986).
We thus conclude that the observed properties of the DNA-water system provide an indication of (may be accounted
by) the coherent molecular dynamics. The theoretical analysis based on quantum electrodynamics (QED) shows
(Montagnier, Aı̈ssa, Del Giudice, et al., 2011) that liquid water appears to behave as an active medium able to
perform through very low frequency electromagnetic fields (e.m.f.). Short range H-bond and electric dipole-dipole
static interactions among liquid water molecules set in as the consequence of the molecule interaction with timedependent radiative e.m.f. over an extended region called coherence domain (CD) (Del Giudice, Doglia, Milani and
Vitiello, 1985, 1986; Del Giudice, Preparata and Vitiello, 1988; Del Giudice and Vitiello, 2006; Bono, Del Giudice,
Gamberale and Henry, 2012). Short range H-bond and electric dipole-dipole static interactions are themselves the
dynamical effects caused by the most fundamental long range molecular and radiative e.m.f. interaction. This last
one is thus responsible for the dynamic origin of short range interactions. This can be better understood by recalling
a few points of the discussion presented in (Montagnier, Aı̈ssa, Del Giudice, et al., 2011).
Above a density threshold and below a critical temperature, an ensemble of molecules interacting with the e.m.f.
undergoes a transition to a dynamical regime characterized by a minimum energy state where the phase oscillations
of the molecules are no longer uncorrelated. Such a minimum energy state implies a configuration of the system
where all molecules enclosed within the CD oscillate in unison in tune with the e.m.f. trapped within the CD (phase
6
ELF
DNA
WATER
(plasma)
DNA
DNA
DNA
DNA
How pathogenic information can persist,
and escape immune defence and treatment.
Figure 6: An hypothetic scheme of the role of EMS in pathogenic DNA amplification.
locking). The linear size of the CD is determined by the wavelength λ of the trapped e.m.f. (typically of the order of
100 nm). The dynamical mechanism ruling the CD formation is the one of the spontaneous breakdown of symmetry
and it is described in (Del Giudice, Doglia, Milani and Vitiello, 1985, 1986; Del Giudice, Preparata and Vitiello,
1988; Del Giudice, Spinetti and Tedeschi, 2010; Del Giudice and Tedeschi, 2009; Del Giudice and Vitiello, 2006).
Its mathematical formulation (Matsumoto, Papastematiou, Umezawa and Vitiello, 1975) is similar to the one of the
Anderson-Higgs-Kibble mechanism (Anderson, 1958; Higgs, 1966; Kibble, 1967) which has led to the recent discovery
of the Higgs particle. One important aspect of such a general QFT mechanism is that the transition to the coherent
dynamical regime can be triggered by a vanishingly weak external input. Due to the weakness of the input, the system
does not get “slaved” by it, but reacts to it according to its own internal dynamics and, provided that the mentioned
conditions of temperature and density are satisfied, the system sets in a coherent state, whose phase is determined by
the phase of the triggering input (Blasone, Jizba and Vitiello, 2011; Umezawa, 1993). Its coherence strength, however,
does not depend on the input strength2 . In the Appendix we will comment on the question whether coherence is not
destroyed by, or theoretically incompatible with the decoherence phenomenon in quantum mechanics (QM). This is
an important issue, which shows that the DNA transduction process is indeed a quantum field theory process; it could
not be understood and described in QM, where the decoherence phenomenon does in fact occur (Alfinito, Viglione
and Vitiello, 2001). Our framework, however, is the one of QFT.
These features already help us in the understanding of some of the experimental observations. In particular, it is
immediately recognized the observed relevance of extremely low signal (ELS) in the phenomena under study. The
stimulation caused by the electromagnetic background of very low frequency is indeed observed to be essential in order
for the DNA-water system to emit the EMS. In the experiments, the background ELS is either produced from natural
sources (the Schumann resonances which start at 7.83 Hz (Montagnier, Aı̈ssa, Del Giudice, et al., 2011; Nickolaenko
and Hayakawa, 2002)) or from artificial sources.
The fragment of DNA embedded in the water also acts as a trigger in the surrounding water, causing the spontaneous
formation of CDs, which appear as a self-produced cavity with molecular coherence strength independent from the
specific input strength. However, there is “phase locking” between the specific DNA molecular structure and the water
molecules. Such a specific feature of the DNA-water coherent coupling accounts for the experimental observations. In
the part of the experiment concerning the DNA transduction, the dynamical phase locking is shared with pure water
in a tube when it is irradiated by the EMS emitted by the aqueous DNA solution system. For brevity, we omit to
report further analysis of the interplay between the size of the CD, the e.m.f. wavelength and the e.m.f. self-trapping
and frequency inside the CD. For the reader convenience, we report in the Appendix a brief summary of some other
features of CD discussed in (Montagnier, Aı̈ssa, Del Giudice, et al., 2011).
The question now naturally arises: does the EMS have any specific property related to the coherent dynamical
structure discussed above? The question is particularly relevant because the emitted EMS, acting on water molecular
dynamics, produces coherent structures such that in PCR processes the DNA transduction occurs with the same
nucleotide sequence as the one of the parent DNA. The answer to the question is provided by observing that the
EMS appears to carry not only the specific information of its frequency spectrum, amplitude and phase modulation
2
We stress that a strong input may drive the system shielding its own internal dynamics. In such a case, the symmetry is said to be
explicitly broken and one has a substantial modification of the original system by inclusion of the strong perturbing agent. However,
this is not what we are interested in in the present case, and in general in Biology, where small perturbing inputs may trigger relevant
reaction of the system driven by its own internal dynamics
7
(the syntactic level), but it also describes the dynamics out of which it is generated. In other words, beside the
syntactic level of pure information (à la Shannon), there is a semantic content, which manifests itself in the underlying
coherent dynamics of the DNA-water system responsible of the polymerization (highly ordered sequence) of hundreds
of nucleotides. We refer to such a semantic content as to the “meaning” of the EMS. In some recent papers it has
been shown that an isomorphism exists between (squeezed) coherent states and self-similar fractal properties (Vitiello,
2009a, 2009b, 2012, 2014). Here, for brevity we do not proceed further with our analysis on this point. Results will
be presented elsewhere.
Above we have mentioned the mechanism of phase locking and phase content in water molecular dynamics. Let
us close this Section by stressing indeed the crucial role played by the phase in the considered processes. Due to
the relation between phase and electromagnetic potential, non-trivial topological properties, with associated BohmAharonov-like effects, have a non-secondary part in the molecular dynamical properties of water (see e.g. (Del Giudice,
Fuchs and Vitiello, 2010; Del Giudice and Vitiello, 2006) and discussions there reported). Such a remark may turn
out to be important when considering charge transfer along the double helix, produced by oxidative agents observed
in (Genereux and Barton, 2010). The question if such a current could contribute to the production of EMS may have
an affirmative answer since the charge transfer along the DNA produces magnetic field in the surroundings. On the
other hand, the oscillations of electric dipoles of the DNA macromolecule may propagate on the DNA in wave form
so to contribute to the electromagnetic signal emission. These EMS are considered in (Del Giudice, Doglia, Milani
and Vitiello, 1985, 1986) where it is shown that they produce symmetry breakdown in the water in which the dipole
chain (DNA or protein chain) is embedded and the mathematical details of the proof are reported.
Finally, concerning the question 1 in Section II.C (how a DNA polymerase, the TAQ polymerase of a thermophilic
bacterium, can ”read” a genetic code on water structures) the whole dynamical scenario above presented provides the
answer to it. It is a complex scenario founded on QFT of coherent systems and is then not surprising that those who
are unaware of it could not conceive the positive answer, with all of its complex but clear details, to that question.
IV.
CONCLUSION AND PERSPECTIVES
This 10 year long collaborative work has yielded some scientific facts and concepts in a new domain of Science at
the frontier of Biology and quantum field Physics.
A new property of some DNA molecules has been discovered, that of emitting low frequency electromagnetic waves
in water dilutions. These DNAs originate from pathogenic agents or agents endowed with pathogenic potential. It
may be not pure coincidence that such EMS are associated with diseases, particularly chronic diseases.
Under natural conditions EMS and water nanostructures may play a role of stealthy elements carrying DNA
information of infectious agents while being undetected by the immune system or being insensitive to conventional
therapies (Fig. 6). However, one cannot discard the possibility that DNA waves can play a role in the physiology of
living entities.
Moreover, in the laboratory, we have shown for the first time that EMS can be re-transcribed into DNA in living
cells. These cells are so far of tumoral origin, opening the way to non-invasive treatments of cancers, assuming that
normal stem cells are not affected, or less affected.
Thus, this new biology that we can call after Jacques Benveniste, Digital Biology, has a very promising future, both
at the level of quantum Physics, and in numerous medical applications.
From the point of view of the theoretical understanding of the observed phenomena, the discussion above presented
suggests that the dynamical law of coherence acts as a law of form inducing guided polymerization processes (controlling morphogenesis): the specific polymerization so obtained is the expression of the semantic content of the EMS
mentioned in the previous Section, by us referred to as the signal meaning. The dynamics of coherence appears to
play the role of dynamic paradigm ruling the ordering (polymerization) processes through dissipative non-equilibrium
dynamics controlled by entropy variations and the consequent appearance of the arrow of time (breakdown of timereversal symmetry) (Celeghini, Rasetti and Vitiello, 1992; Vitiello, 2012, 2014).
The experiments discussed in this paper suggest that also in the usual PCR processes the DNA duplication is
obtained due to the EMS emitted by the parent DNA in the environment of reciprocal inter-actions with water
molecules, enzymes, primers and nucleotides in the solution.
The EMS appears thus to be the carrier of the coherence expressed in the DNA code. One might conjecture (Vitiello,
2014) that modifications are induced in the properties of the EMS resulting in the “deformation”3 of coherence (e.g.
3
In the jargon of coherent states, the word deformation, or also q-deformation, refers to the technically well defined process of “squeezing”
of the coherent state. For technical details see (Yuen, 1976; Celeghini, De Martino, De Siena, et al. 1995).
8
such as those, but not only those, induced by the observed bacterial actions; cf. Section II.B). This may play a
role in epigenetic modifications, thus revealing the appearance of “new meanings” (in the above mentioned sense)
associated to deformed properties of EMS. DNA appears to be the vehicle through which coherence and its dynamical
deformations propagate in living matter (Vitiello, 2014).
Acknowledgements
Partial financial support from MIUR and INFN is acknowledged. We thank Pr. E. Schutz, and Dr. H. Urnovitz,
Chronix Biomedical, for allowing transduction experiments in their laboratory. Mrs. Laila Aı̈ssa is acknowledged for
her skilled technician assistance. Mrs. S. McDonnell is acknowledged for her constant participation in the management
of this work.
Appendix A: On some properties of water molecular dynamics
One of the peculiarities of water consists in the fact that water molecules in the CD coherently oscillate between the
ground state and an excited state lying at 12.06 eV, just below the 12.60 eV ionization threshold (Bono, Del Giudice,
Gamberale and Henry, 2012; Montagnier, Aı̈ssa, Del Giudice, et al., 2011). The almost free electrons in the CD can
be excited by external inputs so to form coherent excitations (vortices), whose entropy is lower than the entropy of
the energy supplied by the inputs. Vortices, due to coherence and their non-trivial topology, are not easily destroyed
by small, additional external supplies of energy. On the contrary, additional energetic inputs may add up to form a
unique vortex, thus storing in the CDs an amount of energy which may be large enough to activate chemical reactions
among molecules, otherwise below the activation energy threshold. Thus, small energy contributions coming from
many high entropy inputs add up to form low entropy ordered patterns of upgraded high energy (Del Giudice, Fuchs
and Vitiello, 2010; Voeikov and Del Giudice, 2009).
An important remark is that DNA and proteins are polyelectrolytes, and are surrounded by positive counterions.
Ions having a cyclotron frequency, νc = q B/(2 π m), where q and m are the electric charge and the mass of the ion,
respectively, and B is the magnetic field (Liboff, 1997; Liboff, Smith and McLeod, 1987), may play an important role
in obtaining a collective performance of water CDs, a coherence of coherent domains. The observed dependence of the
signal emission on the aqueous dilution may be understood (Montagnier, Aı̈ssa, Del Giudice, et al., 2011) as follows:
suppose that a low magnetic field (for example a natural or artificially produced background magnetic field) matches
the ion cyclotron frequency; suppose it may be then able to extract n ions per CD. Then, due to angular momentum
conservation, the plasma of N quasi-free electrons in the CDs starts to counter-rotate with a frequency much higher
than the ion cyclotron frequency since electron mass is much smaller than the ion mass. This frequency depends on
the number of involved ions, namely on their concentration, which therefore is the only relevant variable. This occurs
on all the CDs of the system, whose number is irrelevant for the frequency purpose, in agreement with observations.
The magnetic component of EMS is produced by the so induced rotation of the plasma of the quasi-free electrons
in the CDs. As a further effect, a co-resonating field appears in the surroundings of the rotating CDs depending on
the ion concentration, i.e. on the DNA solution dilution. It could be at the origin of an extended coherence among
CDs. The existence of the observed window of dilutions for the occurrence of the EMS emission could be understood
by presuming that the signal produced by the lower dilutions could have a frequency higher than the interval of the
values detectable by the used instruments. Higher dilutions, on the contrary, could produce no signal because the ion
concentration is decreased below the threshold able to excite the CDs (Montagnier, Aı̈ssa, Del Giudice, et al., 2011).
We observe that thermal collisions could be in competition with electrodynamic attraction of molecules inside the
CD and produce permanent fluxes of molecules between a coherent regime and a non-coherent one, and vice-versa,
although the total number of coherent and non-coherent molecules are constant for a given temperature T. Water is
thus not a homogeneous liquid, rather it appears as a two fluid system, with coexisting coherent and non-coherent
phases, like in the Landau theory of liquid Helium (Landau, and Lifshitz,1959). We have thus a mixed structure
system, consistent also with experimental findings (Taschin, Bartolini, Eramo et. al. 2013), which may appear in
observations only when observation time is very short with respect to the time of flickering between the two phases.
Near surfaces, the coherent phase may be more stable due to the interaction between water molecules and the surface
(Pollack, 2001, 2013). For example, in living matter, water, which is bound to membranes or to biomolecules, could
more easily manifest the properties of coherence.
Let us finally consider the question whether the phenomenon of decoherence in quantum mechanics (QM) might
contradict our analysis based on the formation and non-vanishing life-time of coherent structures in water. We
remark that the belief that coherence is possible only at very low temperatures is disproved by the fact that coherence
is observed in a wide range of temperature, from very low to very high ones: the diamond crystal loses its coherence
9
(it melts) at a temperature of about +3545 0 C in the absence of oxigen; the common kitchen salt N aCl melts at
+804 0 C; in the iron the coherence of the elementary magnets is lost at +770 0 C. In superconductors the critical
temperature Tc are much lower, in some compound of niobium they are not higher than −252 0 C and for some high Tc
superconductors it is a little above −153 0 C. Also the so called BEC systems (which are mostly condensates of atoms)
need very low temperatures and are not so stable. All these systems are macroscopic systems and they are described
in quantum field theory in terms of coherent Bose-Einstein condensates. The bosons that condense in a crystal are
called the phonons, i.e. the quanta of the elastic waves responsible of the ordering in crystals; in the magnets, they
are called the magnons, namely the quanta of the spin waves in magnets; in the water, they are called “dipole wave
quanta” (DWQ), the quanta of the fluctuating molecular dipole waves; and so on. This teaches us that the ordered
patterns we observe at a macroscopic scale in these systems are sustained and generated by long range correlations
maintained by these waves (Alfinito, Viglione and Vitiello, 2001). One would never be able to construct any of these
systems by using short range interaction among the nearest neighbours. Short range interaction, if it is there, is made
possible by the long range one which brings “near” the components (e.g., making possible the formation of H-bonds
in water). Decoherence in QM would forbid the existence of crystals, magnets, superconductors, etc.. However, these
systems do exist and are observed since they are QFT systems.
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