Download erma application internal cover sheet

ERMA APPROVAL CODE
MASSEY UNIVERSITY
GENETIC TECHNOLOGY COMMITTEE
COVER SHEET
Name of Applicant:
Massey University
Principal Investigator:
(Staff Member only)
Title of Project:
(copy and paste this title to the cover page of the ERMA application form under ‘Application Title’)
MAF Facility number:
Laboratory room number(s):
Containment level applicable to this lab(s):
MAF Standard(s) applicable to this lab(s):
Brief Description of Project in Lay Terms:
(copy and paste your description, formatted as a paragraph rather than a table, to the ‘Lay Summary and Project Description
Summary’ section of the ERMA application form)
BACKGROUND
(optional)
WHAT
WHY
HOW
JUSTIFY
(Why is GM
needed?
Why is project
important?)
PROJECT
SCOPE
(Host/Donor
restrictions
- if applicable)
Examples of brief descriptions in lay terms using this format are provided on the following pages.
Please do not include these examples with your submission to the GTC!
Fate of Organism:
CONTINUED OVER
Examples of Brief Project Descriptions in Lay Terms (please delete this page):
BACKGROUND
(optional)
WHAT
This project seeks approval to clone genes (as cDNAs) derived from cyanobacteria and
photosynthetic eukarya
WHY
to study the evolution, function and control of primary metabolic pathways in plants.
HOW
DNA will be sequenced, and proteins will be expressed from recombinant DNA. E. coli or
P. pastoralis containing genes from photosynthetic eukarya will be used to compare
enzyme functions in primary metabolic pathways, e.g., reductive sulphur assimilation,
ethylene biosynthesis, where differences occur across the plant kingdom and
cyanobacteria, or between higher and lower taxonomic groups.
JUSTIFY
(why GM
needed?
why project
important?)
Use of transgenic hosts enables comparative gene family or evolutionary-based studies on
enzyme groups with similar function—an increasingly widely used and important tool in
determining metabolic regulation.
PROJECT
SCOPE
(Host/Donor
restrictions
- if applicable)
Genes derived from NZ native biota or from organisms identified under the Convention on
International Trade in Endangered Species (CITES) will be excluded, as will genes that may
increase the pathogenicity, virulence or infectivity of the host organism, or its ability to
escape containment.
BACKGROUND
(optional)
WHAT
WHY
HOW
JUSTIFY
(why GM
needed?
why project
important?)
PROJECT
SCOPE
(Host/Donor
restrictions
- if applicable)
The green blowfly Lucilia sericata is one of four blowfly species associated with sheep
flystrike in New Zealand. However, this species is also used in medical applications of
sterile maggots to treat wounds that do not respond well to antibiotics.
This project seeks approval to produce transgenic strains of Lucilia sericata
to study the genes that are required for early development of L. sericata, and genes that
may encode factors important in wound healing.
Initially, we will determine if methods developed in Australia for making transgenic L.
cuprina can be applied to L sericata. If so, we will use this technology to integrate various
gene constructs including reporter genes, L. sericata genes with epitope tags and hairpin
RNAs to induce RNA interference and knock-down gene expression.
Use of transgenic strains of L. sericata will enable us to identify and characterise genes
essential for early development and wound healing. This study could lead to the
identification of new targets for novel blowfly-specific insecticides that would be more ecofriendly than the broad-spectrum insecticides in current use. It could also improve the
effectiveness of the use of sterile maggots in promoting wound healing.
- Please delete this page -
Name of the
organism
and
taxonomic
authority:
If more than
one, please
list as shown
in the
example on
the next
page
Specify the
category of host
organism (e.g.
category 1 or 2)
as outlined in the
new HSNO LowRisk Genetic
Modification
Regulations 2003
If host is a
micro-organism
in category 2,
please also state
if it is risk group
1 or 2
What the
organism is
modified with:
Please specify
vector and donor
DNA.
Category
requirements
(e.g. Category
B) as outlined in
the new HSNO
Low-Risk
Genetic
Modification
Regulations
2003
Containment
level (e.g.
PC1, PC2) as
in the AS/NZS
Standard
2243.3 2002
Safety in
Laboratories
Pt 3:
Microbiological
aspects and
containment
facilities
Examples of the information required are provided on the following pages. Please do not include these
examples with your submission to the GTC! Please note that the information requested in this table is pasted
directly into the decision form that is submitted to ERMA.
Please attach this Cover Sheet to the ERMA application and send:
(i) one hard copy to the Secretary, GTC, Research Ethics Office, and
(ii) the electronic file for this cover sheet to [email protected]
Name of the
organism
and
taxonomic
authority:
If more than
one, please
list as shown
in the
example
below.
Examples:
Candida
albicans
(Berkhout,
1923)
Escherichia
coli (non
pathogenic
strains)
Migula 1895
(Castellani &
Chalmers
1919)
Escherichia
coli strain
K12
derivatives
Migula 1895
(Castellani &
Chalmers
1919)
Specify the
category of host
organism (e.g.
category 1 or 2)
as outlined in the
new HSNO LowRisk Genetic
Modification
Regulations 2003
What the
organism is
modified with:
Please specify
vector and donor
DNA.
Category
requirements
(e.g. Category
B) as outlined
in the new
HSNO LowRisk Genetic
Modification
Regulations
2003
Containment level
(e.g. PC1, PC2) as
in the AS/NZS
Standard 2243.3
2002 Safety in
Laboratories Pt 3:
Microbiological
aspects and
containment
facilities
Category B
PC2
Category A
PC1
Category A
PC1
If host is a
micro-organism
in category 2,
please also state
if it is risk group
1 or 2.
Host category 2
Risk group 2
Host category 1
Host category 1
Integrative
vectors
containing
Saccharomyces
cerevisiae genes
Non-conjugative
vectors
containing
Candida albicans
DNA
Non-conjugative
vectors
containing
Drosophila
melanogaster
DNA, reporter
genes, selectable
markers and may
have regulatory
or localisation
sequences fused
to them such as
promoters, nonDrosophilid 2
component
systems for
controlling
expression,
protein/epitope
tags, transposable
elements and
polyA signals
Name of the
organism and
taxonomic
authority:
If more than
one, please list
as shown in the
example below.
Examples:
Insect cell lines
from
Drosophila
melanogaster
Drosophila
melanogaster
(Meigen, 1830)
Specify the
category of host
organism (e.g.
category 1 or 2)
as outlined in the
new HSNO LowRisk Genetic
Modification
Regulations 2003
What the
organism is
modified with:
Please specify
vector and donor
DNA.
Category
requirements
(e.g. Category
B) as outlined
in the new
HSNO LowRisk Genetic
Modification
Regulations
2003
Containment
level (e.g. PC1,
PC2) as in the
AS/NZS
Standard 2243.3
2002 Safety in
Laboratories Pt
3:
Microbiological
aspects and
containment
facilities
Category A
PC1
Category B
PC2
If host is a
micro-organism
in category 2,
please also state
if it is risk group
1 or 2.
Category 1
Category 2
Vectors containing
Drosophila
melanogaster
DNA, reporter
genes, selectable
markers and may
have regulatory or
localisation
sequences fused to
them such as
promoters, nonDrosophilid 2
component systems
for controlling
expression,
protein/epitope
tags, transposable
elements and polyA
signals
Transformation
vectors containing
Drosophila
melanogaster
DNA, reporter
genes, selectable
markers and may
have regulatory or
localisation
sequences fused to
them such as
promoters, nonDrosophilid 2
component systems
for controlling
expression,
protein/epitope
tags, transposable
elements and polyA
signals