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Transcript
Figure S2. NELF-E potentiates expression of the slp1[PESE]-lacZ reporter. Fluorescent double in situ hybridization was used to compare the expression of a reporter gene containing a slp1 cis-regulatory element extending from 3.9 to 1.8 kb upstream of the slp1 promoter fused to a 129 bp slp1 basal promoter, followed by lacZ with that of the endogenous slp1 mRNA (green). (A) schematic diagram of the slp1[PESE]-lacZ reporter, labeled as in Figure 4. (B) lacZ expression (red) in wild-type gastrula stage embryos is observed only in cells corresponding to the even-numbered slp1 stripes. (C) reporter gene expression is reduced in NELF-E[PB] germline clone embryos.
