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Name Class Date 13.4 Gene Regulation and Expression Prokaryotic Gene Regulation 1. How do prokaryotes conserve energy? 2. How do DNA-binding proteins in prokaryotes regulate genes? 3. What is an operon? 4. Complete the table to describe the role of each regulatory region or molecule in the operation of the lac operon. Regulatory Region or Molecule What It Does Repressor protein Operator RNA polymerase Lactose Eukaryotic Gene Regulation 5. In what two ways is gene regulation in eukaryotes different from gene regulation in prokaryotes? a. b. 6. What is a TATA box? What does a TATA box do? 7. What are transcription factors and what do they do? 8. Explain how gene regulation makes cell specialization possible. 9. What is microRNA and how is it related to mRNA? Genetic Control of Development For Questions 10–16, write the letter of the correct answer on the line at the left. 10. As an embryo develops, different sets of genes are regulated by A. mRNA and lac repressors. C. transcription factors and repressors. B. operons and operators. D. promoters and operators. 11. The process through which cells become specialized in structure and function is A. transcription. C. differentiation. B. gene expression. D. RNA interference. 12. Homeotic genes are A. regulator genes that bind to operons in prokaryotes. B. master control genes that regulate organs that develop in specific parts of the body. C. parts of the silencing complex that regulates gene action through RNA interference. D. base sequences complementary to sequences in microRNA. 13. What role do homeobox genes play in cell differentiation? A. They code for transcription factors that activate other genes important in cell development and differentiation. B. They block certain gene expression. C. They cut double-stranded loops into microRNA. D. They attach to a cluster of proteins to form a silencing complex, which binds to and destroys certain RNA. 14. In flies, the group of homeobox genes that determines the identities of each segment of a fly’s body is the group known as A. silencing complexes. C. operators. B. promoters. D. Hox genes. 15. Clusters of Hox genes are found in A. flies only. C. plants only. B. flies and frogs only. D. nearly all animals. 16. The “switches” that trigger particular patterns of development and differentiation in cells and tissues are A. mRNA molecules. C. silencing complexes. B. master control genes. D. Dicer enzymes.