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SLUH – Bio 431/AP Bio – Pham Reading Guide 11 RG 11 – Regulation of Gene Expression* *Adapted and modified from Robbyn Tuinstra’s work 11.1 – Strategies of Regulating Gene Expression 1. Distinguish between constitutive and inducible genes. 2. Examine Fig. 11.2, differentiate negative regulation and positive regulation. 3. What are viruses? Explain why they do not qualify as organisms. 4. Listed below are the steps in the lytic cycle of viruses. Put the steps in the correct order. _____ Phage genome directs host cell to produce phage components (DNA and capsids) _____ Self assembly of phage _____ Bacteriophage attaches to host cell _____ Host cell lyses releasing phage particles _____ Hydrolytic enzymes destroy host cell’s DNA _____ Phage injects DNA into host cell 5. Distinguish the lytic and the lysogenic cycles. 6. Speculate the advantage(s) of a lysogenic cycle. 7. Explain how the replication of retroviruses (like HIV) is different from that of other viruses. 8. Listed below are the steps in the replication of a retrovirus. Put the steps in the correct order. _____ Attachment of virus _____ Reverse transcription – Viral RNA used as template to produce minus strand of DNA _____ Uncoating of single-stranded RNA genome _____ Proviral DNA transcribed into mRNA; mRNA translated into proteins and may become genome for next generation of viruses. _____ Capsid proteins enzymatically removed. _____ Virus enters host cell cytoplasm _____ Integration – newly produced viral DNA enters nucleus, inserts into host DNA and becomes provirus. Section 11.2 – Prokaryotic Regulation of Gene Expression Page 1 of 3 SLUH – Bio 431/AP Bio – Pham Reading Guide 11 9. Explain – and provide the reason for – the primary/most efficient way prokaryotes regulate gene expression. 10. Discuss the benefit of such method of gene regulation vs. than allosteric inhibition of enzyme activity. 11. What is an operon? 12. Study Fig. 11.8, explain how the lac operon works. a. If lactose is absent from the cell b. If lactose is present in the cell 13. Study Fig. 11.9, explain how the trp operon works. a. If tryptophan is absent from the cell b. If tryptophan is present in the cell 14. Explain how tryptophan acts as a corepressor and why the trp operon is an example of a repressible operon. Section 11.3 – Eukaryotic Regulation of Gene Expression 15. Summarize the differences in transcription between prokaryotes and eukaryotes. 16. Are all possible genes expressed in every possible cell in a eukaryotic organism? Explain. 17. In prokaryotes, does translation immediately follow transcription? Why? 18. How does the presence of a nucleus allow eukaryotes to regulate gene expression differently than prokaryotes? (Why can’t prokaryotes regulate gene expression beyond the transcriptional level?) TRANSCRIPTIONAL CONTROL 19. Define: TRANSCRIPTIONAL ACTIVATOR TRANSCRIPTION FACTOR PROMOTER ENHANCER Page 2 of 3 SLUH – Bio 431/AP Bio – Pham Reading Guide 11 20. How is it possible that a promoter or enhancer can be located a long distance away from the start of a gene? CHROMATIN STRUCTURE, CHEMICAL MODIFICATION OF DNA AND GENE EXPRESSION 21. Explain DNA methylation. What is the effect of methylation? 22. Describe the two chemical modifications that can occur to histone proteins. 23. What is a Barr body? How is a Barr body an example of genetic inactivation by chromatin structure? 24. Define epigenetic inheritance. 25. Define genetic imprinting. Section 11.4 – Post Transcriptional Control of Gene Expression 26. List the various modifications that must be made to an mRNA before it can leave the nucleus. 27. How does alternative splicing of introns effect the final gene product? 28. What can hinder the transport of mRNA from the nucleus? TRANSLATIONAL CONTROL 29. What determines how long a mRNA will remain in the cytoplasm? 30. Give an example of posttranscriptional control of gene expression. POSTTRANSLATIONAL CONTROL 31. Why are some proteins not immediately active after synthesis? Provide an example. 32. What regulates the half-life of a protein in a cell? Give an example of a short-lived protein. Page 3 of 3