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Download What Is Gene cloning and How Is It Used? 1. Explain what is meant
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Transcript
What Is Gene cloning and How Is It Used? 1. Explain what is meant by the term "gene cloning" and indicate the main goals of this procedure. 2. Regarding the use of vectors in gene cloning: Indicate the purpose of the vector and name the two types of vectors used. Define the term "restriction enzymes" and explain how they are used to insert genes into a vector. Explain one way in which a specific gene can be identified within a bacterial colony. 3. Define the term "DNA library" and indicate (in general terms) how one is made. 4. Describe how the polymerase chain reaction (PCR) is used to copy DNA; define the terms thermocycling, primers, and hybridization. 5. Regarding applications of biotechnology: Explain why PCR has become the method of choice in forensic DNA fingerprinting. Indicate how recombinant bacteria have been used as "chemical factories' and why this is important in the medical field. Explain the role the recombinant bacteria may play in bioremediation. Define the term "gene therapy" and indicate how it might be used in the future.
