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Topic 3: The Chemistry of Life 1. Assessment Statement Response Campbell pages 3.1 Chemical elements and water: 2 hours 3.1.1 State that the most Pg. 33 frequently occurring chemical elements in living things are hydrogen, oxygen, carbon, and nitrogen 3.1.2 State that a variety of Pg. 33 other elements are needed by living organisms, including sulfur, calcium, phosphorus, iron, and sodium 3.1.3 State one role for each The major roles of these elements can be found of the elements throughout the textbook. mentioned in 3.1.2 IB says: Refer to the roles in plants, animals, and prokaryotes Sample Test Questions What element occurs in all proteins? Nitrogen What role does sulfur play in living organisms? D. Component of proteins 3.1.4 Draw and label a diagram showing the structure of water molecules to show their polarity and hydrogen bond formation 3.1.5 Outline the thermal, cohesive, and solvent properties of water Pg 47-48 Draw and label a diagram, etc. Pg. 48-52 Outline the thermal properties of water. 3.1.6 Explain the relationship Pg. 48-52 between the properties of water and its uses in IB says: Limit the properties to those outlined in 3.1.5 living organisms as a coolant, medium for metabolic reactions, and transport medium. 3.2 Carbohydrates, lipids, and proteins: 2hrs 3.2.1 Distinguish between Pg 58. Campbell defines “organic compounds” as organic and inorganic “compounds containing carbon.” IB disagrees with that compounds definition IB says: Compounds containing carbon that are found in living organisms (except hydrogen-carbonates, carbonates, and oxides or carbon) are regarded as organic. 3.2.2 Identify amino acids, glucose, ribose and fatty acids from diagrams showing their structure Pg. 70-78 IB says: Specific names of amino acids and fatty acids are not expected. 3.2.3 List three examples each of monosaccharides, disaccharides, and polysaccharides. IB says: The examples used should be: glucose, galactose, and fructose; maltose, lactose, and sucrose; and starch, glycogen, and cellulose. 3.2.4 State one function of Pg. 70-72, does not include function of fructose glucose, lactose, and glycogen in animals, and of fructose, sucrose, and cellulose in plants. 3.2.5 Outline the role of condensation and hydrolysis in the relationships between monosaccharides, disaccharides and polysaccharides; between fatty acids, glycerol and triglycerides; and between amino acids and polypeptides. Pg. 68-69 The relationships between condensation and hyrdrolysis reactions and the formation and disassembly of macromolecules is discussed in general though not with all the specific molecules at left. Campbell uses the term “dehydration reaction” in some places instead of “condensation”. These terms refer to the same thing. IB says: This can be dealt with using equations with words or chemical formulas. 3.2.6 State three functions of lipids Pg. 76-77 IB says: Include energy storage and thermal insulation. 3.2.7 Compare the use of carbohydrates and lipids in energy storage Can be inferred from 70-76 May 2006 PM (SL) 7c. Describe why carbohydrates and lipids are used as energy stores (c) carbohydrates and lipids contain a lot of chemical energy; carbohydrates are readily used in cell respiration / sugars are quick access energy stores; lipid molecules contain about twice as much energy as carbohydrates; complex carbohydrates/polysaccharides/starch/glycogen are also long term energy stores; lipids are long term energy reserves; complex carbohydrates/polysaccharides/starch/glycogen and lipids are insoluble / will not diffuse out of cells; complex carbohydrates/polysaccharides/starch/glycogen/lipids do not contribute (significantly) to osmotic pressure; complex carbohydrates/polysaccharides/starch/glycogen can be converted into sugars by hydrolysis; carbohydrates and lipids burn cleaner than proteins / do not yield N waste; [6 max] (Plus up to [2] for quality) 3.3 DNA structure: 1 hour 3.3.1 Outline DNA nucleotide structure in terms of sugar (deoxyribose), base, and phosphate. 3.3.2 State the names of the four base pairs of DNA 3.3.3 Outline how DNA nucleotides are linked together by covalent bonds into a single strand Pg. 87 IB says: Chemical formulas and the purine/pyrimidine subdivision are not required. Simple shapes can be used to represent the component parts. Only the relative positions are required. Pg 88 Pg 88 IB says: Only the relative positions are required. 3.3.4 Explain how a DNA double helix is formed using complementary base pairing and hydrogen bonds. Pg 88 3.3.5 Draw and label a simple diagram of the molecular structure of DNA Can be inferred from pages 87 and 88. IB says: An extension of the diagram in 3.3.3 is sufficient to show the complementary base pairs of A-T and G-C held together by hydrogen bonds and the sugarphosphate backbones. The number of hydrogen bonds between pairs and details of purine/pyrimidines are not required. 3.4 DNA Replication: 1 hour 3.4.1 Explain DNA replication in terms of unwinding the double helix and separation of the strands by helicase, followed by the formation of new complementary strands by DNA polymerase. Pg. 89 Helicase and DNA polymerase are not mentioned until pages 300-304 (Concept 16.2) IB says: It is not necessary to mention that there is more than one DNA polymerase. 3.4.2 Explain the significance Pg. 89 of complementary base pairing in the conservation of the base sequence of DNA 3.4.3 State that DNA Pg. 299 replication is semiconservative 6 May 2009 PM 6b Explain the process of DNA replication. [8] 2009 Specimen Paper 6. The diagram shows part of a molecule produced by replication of DNA. What is the significance of the shaded and the unshaded regions? (One strand is shaded and the complementary strand is unshaded) A. The shaded parts are DNA and the unshaded parts are mRNA. B. The shaded parts contain adenine and thymine and the unshaded parts contain guanine and cytosine. C. The shaded part is a codon and unshaded part is an anticodon. D. One of the parts has been newly synthesized and the other was part of a pre-existing DNA molecule. 3.5 Transcription and Translation: 2 hours 3.5.1 Compare the structure Pg. 88 of RNA and DNA IB says: Limit this to the names of sugars, bases, and the number of strands. 3.5.2 Outline DNA transcription in terms of the formation of an RNA strand complementary to the DNA strand by RNA polymerase Pg. 311-315 6 May 2009 PM 6a Distinguish between DNA and RNA [3] May 2006 PM (SL) 5. (a) Determine the strand of mRNA that is transcribed from the DNA strand below. A–T–C–C–A–G–G–T–C–A–A–G [1] (b) List three of the other molecules, apart from mRNA, required for transcription. [3] 5. (a) – U – A – G – G – U – C – C – A – G – U – U – C – [1] (b) DNA; RNA polymerase; (ribose) nucleotides / ribonucleotides / RNA nucleotides; transcription factors; nucleoside/ribonucleoside triphosphates; Any two of the following: A / C / G / U; [3 max] 3.5.3 Describe the genetic code in terms of codons composed of triplets of bases. Pg 314 3.5.4 Explain the process of translation, leading to polypeptide formation. Pg 320-324 2009 Specimen Paper IB says: Include the roles of messenger RNA (mRNA), transfer RNA (tRNA), codons, anticodons, ribosomes and amino acids. 7. If mRNA has a codon CAU, what is the corresponding anticodon on the tRNA molecule? A. CAT B. GUA C. CAU D. GTA 3.5.5 Discuss the relationship between one gene and one polypeptide. Pg. 310 IB says: Originally, it was assumed that one gene would invariably code for one polypeptide, but many exceptions have been discovered. 3.6 Enzymes: 2 hours 3.6.1 Define enzyme and active site 3.6.2 Explain enzymesubstrate specificity Pg. 150 Pg. 152 4 May 2006 PM (SL) IB says: The lock-and-key model can be used as a basis for the explantion. Refer to the three dimensional structure. The induced-fit model is not expected at SL. 4. (a) State why each step in a biochemical pathway often requires a separate enzyme. [2] 4. (a) enzymes are specific for their substrate / lock and key model / energy requirements for reactions with substrates vary; each step of the pathway is unique / different substrate at each step; finer control of metabolic pathways; [2] 3.6.3 Explain the effects of temperature, pH, and substrate concentration on enzyme activity Pg. 154 I did not find a mention in Campbell of how substrate concentration affects enzyme activity. 8. Which graph shows the relationship between the substrate concentration and the rate of an enzyme controlled reaction? May 2006 PM (SL) 4(b) Explain the effects of either changing temperature or pH on enzyme activity. [3] (b) Either, temperature: [3 max] each enzyme has an optimal temperature for its maximum activity; (small) temperature increases result in increased enzyme activity to a point/optimum; increase activity due to increased movement of molecules / increased kinetic energy or conversely stated; temperature increases above the optimum causes (progressive) loss of activity due to denaturation/shape changes or, pH: [3 max] each enzyme has an optimal pH for its maximum activity; as pH varies from optimal pH, enzyme activity diminishes / becomes inhibited; loss of activity is due to denaturation/shape changes; gain or loss of hydrogen ions distorts tertiary shape of enzyme; homeostatic mechanisms maintain optimal conditions for enzyme activity; [3 max] Credit marking points above if illustrated by a suitably annotated graph. 3.6.4 Define denaturation Pg 84 IB says: Refer only to heat and pH as agents. 3.6.5 Explain the use of lactase in the production of lactosefree milk 3.7 Cell respiration: 2 hours 3.7.1 Define cell respiration 3.7.2 State that, in cell respiration, glucose in the cytoplasm is broken down by glycolysis into pyruvate, with a small yield of ATP. Not discussed in Campbell. Pg. 161 Important: Campbell uses the term “cellular respiration” to refer ONLY to aerobic respiration. IB uses the term “cell respiration” to refer to both aerobic and anaerobic respiration. Pg. 165 3.7.3 Explain that, during anaerobic respiration, pyruvate can be converted in the cytoplasm into lactate, or ethanol and carbon dioxide, with no further yield of ATP. Pg. 175 IB says: Mention that ethanol and carbon dioxide are produced in yeast, whereas lactate is produced in humans. 3.7.4 Explain that, during aerobic cell respiration, pyruvate can be broken down in the mitochondria into carbon dioxide and water with a large yield of ATP. Pg. 168-174 3.8 Photosynthesis: 3 hours 3.8.1 State that photosynthesis involves the conversion of light energy into chemical energy. 2009 Specimen Paper 9. Humans can respire aerobically and anaerobically. Which are products of both aerobic cell respiration and anaerobic cell respiration in humans? A. pyruvate and ATP B. pyruvate and lactate C. ATP and carbon dioxide D. lactate and carbon dioxide Pg. 181 3.8.2 State that light from the Sun is composed of a range of wavelengths (colours). Pg. 186 3.8.3 State that chlorophyll is the main photosynthetic pigment. 3.8.4 Outline the differences in absorption of red, blue, and green light by chlorophyll. Pg. 182 IB says: Reference to actual wavelengths or frequencies is not expected. Pg. 187-188 IB says: Students should appreciate that pigments absorb certain colors of light. The remaining colors of light are reflected. It is not necessary to mention wavelengths or the structure responsible for the absorption. 3.8.5 State that light energy is Pg. 184-185 used to produce ATP, and to split water molecules (photolysis) to form oxygen and hydrogen. 3.8.6 State that ATP and Pg. 184-185 hydrogen (derived from the photolysis of water) are used to fix carbon dioxide to make organic molecules. 3.8.7 Explain that the rate of photosynthesis can be measured directly by the production of oxygen or the uptake of carbon dioxide, or indirectly by an increase in biomass. Not specifically discussed in Campbell. IB says: The recall of details of specific experiments to indicate that photosynthesis has occurred or to measure the rate of photosynthesis is not expected. 3.8.8 Outline the effects of temperature, light intensity, and carbon dioxide concentration on the rate of photosynthesis. Not specifically discussed in Campbell May 2006 PM (SL) 7. (b) Explain how the rate of photosynthesis can be measured. [7] (b) CO2 +H2O (CH2O)n + O2 / suitable photosynthesis equation; amount of CO2 absorbed (per unit time) can be measured; measuring the increase of biomass (per unit time); O2 excretion (per unit time) can be measured; methods for measuring the above: volume of O2 (bubbles) produced per unit time can be measured; dry mass can be weighed; increase in starch concentration in leaves (as measured by iodine); use of pH indicator can monitor CO2 uptake in water; the rate of photosynthesis measured is relative because some of the CO2 is produced by the plant internally through respiration; the rate of photosynthesis measured is relative because some of the carbohydrates are used internally by the plant for respiration; [7 max] 2009 Specimen Paper 5(b) Outline the role of chlorophyll and the effects of temperature, light intensity and carbon dioxide concentration on the rate of photosynthesis. [6] (b) chlorophyll is composed of a number of pigments; absorb different colours of light; mainly red and blue absorbed; green light reflected; temperature increases rate; up to a point where enzymes denature; light intensity increases rate; up to a point where maximum absorbance can occur; carbon dioxide increases rate; up to a point where fixation is at a maximum; [6 max]