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Transcript
LECTURE 2: An Overview of Bacterial Cell Structure Viro102: Bacteriophages & Phage Therapy 3 Credit hours NUST Centre of Virology & Immunology Cell types Prokaryotic Cell Bacteria & Archaea Eukaryotic Cell animals, plants, fungi Bacterial Cell Morphology Bacterial Cell no membrane bound organelles Capsule Cell Wall Cell membrane Ribosome Plasmids Chromosome Flagellum Pilli Capsule Outer most covering of some bacteria Well organized layer, not easily washed off Helps to protect bacteria against o Phagocytosis, A capsule specific antibody may be required for phagocytosis to occur o Bacteriophages o They also exclude bacterial viruses and o Hydrophobic toxic materials such as detergents Capsule Capsules also contain water which protects bacteria against desiccation Allow bacteria to adhere to surfaces and cells. Composed of polysaccharides Bacillus Anthracis has protinaceous capsule composed of poly-D glutamic acid Slime Layer Glycocalyx Bacterial Cell no membrane bound organelles Capsule Cell Wall Cell membrane Ribosome Plasmids Chromosome Flagellum Pilli A comparison b/w G(+ve) & G(-ve) GRAM POSITIVE Lipoteichoic acid Peptidoglycan-teichoic acid Cytoplasmic membrane Cytoplasm GRAM NEGATIVE Porin Outer Membrane Braun lipoprotein Inner (cytoplasmic) membrane Cytoplasm Lipopolysaccharide Classification of Bacteria In 1884, Hans Christian Gram developed a staining technique for the identification of bacteria Gram staining Based on this technique, Bacteria is characterized into o Gram positive o Gram negative The difference is due to the cell wall differences in these bacteria Gram Staining Technique What happens in Gram staining? Crystal Violet (purple) o Primary stain; positive stain. o Stains all cell walls purple. Iodine o Mordant (Any substance used to facilitate the fixing of a dye to a fibre). o Combines with CV to form an insoluble complex that gets trapped in thicker peptidoglycan layers. Ethanol o Decolorizer. o CV complex washed out of Gram negative organisms because it cannot be trapped by outer layer. Safranin (pink) o Counterstain. o Stains all cells, but only the negative ones actually appear pink. Prepare a thin smear of fresh bacteria Apply primary stain & then wash Apply mordant & then wash Visualize Apply secondary stain & then wash Apply ethanol Results under a Microscope Yogurt: (Lactobacillus {G+} and Enterobacteria{G-}) G -ve Bacillus G +ve Gram stain of cerebrospinal fluid showing B. anthracis Bacterial Cell No membrane bound organelles Capsule Cell Wall Cell membrane Ribosome Plasmids Chromosome Flagellum Pilli Cell Membrane Composition: o Phospholipid bilayer just like eukaryotic PM (Fluid Mosaic) o Lipids are not homogeneously distributed in membrane o Contain fatty acids with additional methyl hydroxyl or even cyclic groups o The relative proportions of these fatty acids can be modulated by the bacterium to maintain the optimum fluidity of the membrane (e.g. following temperature change) Cell Membrane Functions: o Acting as a permeability barrier for most molecules o Serving as the location for the transport of molecules into the cell o Function in energy production as the location about which a proton motive force is generated o Act as site for photosynthesis in Cyanobacteria Bacterial Cell No membrane bound organelles Capsule Cell Wall Cell membrane Ribosome Plasmids Chromosome Flagellum Pilli Ribosomes Site of protein synthesis Made up of protein and RNA The 70S ribosome is made up of a 50S and 30S subunits The 50S subunit contains the 23S and 5S rRNA The 30S subunit contains the 16S rRNA Bacterial Cell No membrane bound organelles Capsule Cell Wall Cell membrane Ribosome Plasmids Chromosome Flagellum Pilli Bacterial Chromosome Present in the cytoplasm The bacterial chromosome is not packaged using histones to form chromatin as in eukaryotes but instead exists as a highly compact supercoiled structure, the precise nature of which remains unclear. Most bacterial chromosomes are circular although some examples of linear chromosomes exist (e.g. Borrelia sp.) Bacterial Cell No membrane bound organelles Capsule Cell Wall Cell membrane Ribosome Plasmids Chromosome Flagellum Pilli Plasmids Most bacteria also contain small independent pieces of DNA called plasmids that often encode for traits that are advantageous but not essential to their bacterial host Bacterial Cell No membrane bound organelles Capsule Cell Wall Cell membrane Ribosome Plasmids Chromosome Fimbriae Pili Flagellum Fimbriae Many prokaryotes have short, fine, hair like appendages that are thinner than flegella known as Fimbriae Interchangeably term fimbraie and pili. Cell may covered by 1000s of fimbraie, visible under electron microscope Cont’d Slender tubes composed of helical arrangement of protein subunits 3-10 nm in diameter Several millimeter long Usually facilitate the attachment of a bacterium to a surface (e.g. to form a biofilm) or to other cells (e.g. animal cells during pathogenesis) Cont’d Moreover help in motility Pseudomonas aeroginosa, Neisseria gonorrhoeae, some strain of E.coli Bacterial Cell No membrane bound organelles Capsule Cell Wall Cell membrane Ribosome Plasmids Chromosome Fimbriae Pili Flagellum Pili Hair like appendages Different from fimbraie Pili are larger 1-10 / cell Genetically determined by conjugative plasmid Phages specifically bind on sex pili, hence infect bacteria Pili During bacterial conjugation, a sex pilus emerging from one bacterium ensnares the recipient bacterium This is direct contact merging the cytoplasms of two bacteria via a controlled pore which allows for the transfer of bacterial DNA from the bacteria with the pilus (donor) to the recipient bacteria Usually the fertility factor is required to produce sex pili Bacterial Cell No membrane bound organelles Capsule Cell Wall Cell membrane Ribosome Plasmids Chromosome Fimbriae Pili Flagellum Flagellum Flagella are whip like structures protruding from the bacterial cell wall and are responsible for bacterial motility Composed of different proteins including flagellin The arrangement of flagella about the bacterial cell is unique to the species observed. Common forms include: Peritrichous Multiple flagella found at several locations on the cell. Polar Single flagella found at one of the cell poles. Lophotrichous A clump of flagella found at one cell pole. Flagellum THANK YOU!