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Transcript
Announcements • Contracts? • Tailgate RSVPs due 14th. • Reminder DRAFT1 due precisely at start of lab both in print & on turnitin.com • Thursday quiz or notebook check in lab • Next week (ie week 4) start you 30 Days experiment outside of lab and continue PCR experiments in lab until succeed. Outside/Prior to lecture • Stand up if completed reading (10 pages) Took Notes, Studied Notes Inside/During lecture • Learning how to use information and knowledge in new situations Outside/Prior to lecture • Stand up if completed reading (10 pages) Took Notes, Answered Qs, Studied Notes Inside/During lecture • Learning how to use information and knowledge in new situations What’s the story, the hypothesis and was it supported? What’s the story, the hypothesis and was it supported? What’s the story, the hypothesis and was it supported? Prepare Paper “Structure” “Function” Pre-test Ribosomes So what does a bacteria cell have & we don’t? Plasmids Cytoplasm Chromosome Flagellum Plasma membrane Cell wall So what does a bacteria cell have & we don’t? A. The plasma membrane surrounding it B. Chromosomes with DNA to make RNA C. Ribosomes making proteins D. The cell wall surrounding it E. None of the above Nucleus Plasma membrane Rough endoplasmic reticulum What does animal cell have that plants don’t? Golgi apparatus Chloroplast Bacteria Cell wall What does animal cell have that plants don’t? A. A single plasma membrane surrounding it B. Mitochondria C. A true Nucleus D. The cell wall surrounding it E. None of the above Antibiotics that inhibit prokaryotic ribosomes Ribosome Antibiotics that inhibit prokaryotic ribosomes A. Will stop bacterial protein synthesis B. Will stop mitochondria protein synthesis C. Will stop nucleus from making proteins D. None of the above E. More than one of the above Collagen Proteoglycan complex EXTRACELLULAR FLUID Fibronectin Integrins Plasma membrane Microfilaments CYTOPLASM Microvillus Plasma membrane Microfilaments (actin filaments) Intermediate filaments 0.25 µm Euchromatin Heterochromatin Nucleolus Nuclear envelope Nucleus 1 µm Nucleolus Chromatin Nuclear envelope: Inner membrane Outer membrane Nuclear pore Pore complex Surface of nuclear envelope Rough ER Ribosome 1 µm 0.25 µm Close-up of nuclear envelope Pore complexes (TEM) Nuclear lamina (TEM) 0.2 m Matrix Cristae Mitochondria 0.2 m Intermembrane space Outer membrane Free ribosomes in the mitochondrial matrix Inner membrane Cristae Matrix 0.1 µm Plasma membrane Cytoplasm Ancestral prokaryote DNA Invagination Endoplasmic reticulum Nuclear envelope Nucleus Aerobic heterotrophic prokaryote Endocytosis Mitochondrion (with extra mem) How to test the endosymbiosis hypothesis… What evidence could you look for? (that might support or refute the hypothesis) Design an experiment to test the endosymbiosis hypothesis… • Maybe using PCR • Maybe using Microscopy • Perhaps using streptomycin, erythromycin, or tetracycline • What’s the experiment, what is your prediction based on the hypothesis? Evidence for Endosymbiosis Mitochondria/Chloroplasts Bacteria Morphology/Behavior: They swim around in your cells, divide, look like cyanobacteria etc Anatomy: Have 2 membranes, inner like bacteria & outer like plasma membrane. Molecular: Their own genes & ribosomes, that function- sequences of genes, ribosomes, same as bacteria, antibiotic, circular no histones Pathogens: Undigested prey or parasites via endo 30