Download Intro to Strawberry DNA Extraction Lab

DNA =
- deoxyribonucleic acid
- The genetic material that is
located in the nucleus of a cell.
- It contains a code for proteins.
Chromosome =
- an organized structure (or body)
made up of twisted up
DNA
- "Chroma" means
color
- "Some" means body
Where have you heard of genes before?
What do genes have to do with DNA?
Gene = Segments of DNA that control the
production of protein
DNA and Chromosomes Entrance Ticket
«grade»
Grade:
«subject»
Subject:
«date»
Date:
1
Which part of the cell contains DNA?
A
Vacuole
B
Ribosome
C
Cell Membrane
D
Nucleus
2
The genetic material that contains a code
for proteins is called...
A
RNA
B
DNA
C
Chromosome
D
Gene
3
When DNA wraps very tightly around itself,
it forms a structure called a(n)...
A
Gene
B
Protein
C
Amino Acid
D
Chromosome
4
DNA within chromosomes are arranged in
segments called...
A
Genes
B
Proteins
C
Sister Chromatids
D
Amino Acids
Strawberry DNA Extraction
Lab
DNA = deoxyribonucleic acid
"Blueprint of Life"
Guides the construction
and development of
living organisms
Isolate and extract DNA:
1. Put cells into special "lysis" solution.
2. Break cells open (mechanically or
chemically).
3. The ingredients in the lysis solution
remove other parts of the cell away from
the DNA.
4. Filter out extra cell parts.
5. Add isopropyl alcohol to precipitate DNA
out of a solution
Lysis Solution
Salt - Na+ has a charge, so does DNA. The DNA and the salt
are attracted to each other and the salt forms a "shield"
around the DNA and helps it clump together.
Lysis Solution
Soap - dishwashing detergent is good at dissolving grease
(which is nonpolar). Like grease, the nonpolar cellular
membranes are dissolved.
Why are we using
strawberries?
·Soft, easy to pulverize
·Already have enzymes inside of them
breaking down cell walls
·Freezing also breaks down cell walls
·Strawberries have lots of DNA!
Procedure
1. Place 1-3 strawberries into a 2. Add 10 mL of the lysis
Ziplock Baggie. Zip closed,
buffer to the baggie, zip again
gently mash.
and mash until it looks like
strawberry jam.
NOTE: This solution has soap
in it. Do not eat.
Procedure
3. Filter the strawberry goo into a beaker using the coffee filter.
You may have to squeeze the juice through (shown below).
4. Pour the filtered solution into a
test tube, filling it about halfway.
Procedure
5. Gently add about 10 mL of chilled isopropyl alcohol to
the test tube. Let it sit for a few minutes until you can see 2
distinct "layers."
6. The white "threads" that you
see forming are strawberry DNA!
Wind them up on a wooden stick
and place on black construction
paper to get a better look.
To Do List for Today:
·Form groups of 3
·Decide the "jobs" that each person will have - supply
collector, observation recorder, strawberry masher, filterer,
etc.
·Read procedure and decide what materials your group will
need
·Have teacher initial this work for points!
·Keep packet in your folder for tomorrow.