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Molecular
Genetics
Evolution
Labs 6-8
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Helicase
__________ unwind the helix and
separates the parent strand at replication forks.
Row 1, Col 1
okazaki fragments which grow 5’  3’ must be joined along the
lagging strand
•HOW DOES DNA SYNTHESIS PROCESS OF THE
LAGGING STRAND DIFFER FROM
THAT OF THE LEADING STRAND?
1,2
REGULATORY GENES
These genes:
CODE FOR REPRESSOR PROTEINS
ARE TRANSCRIVED CONTINUOUSLY
ARE NOT CONTAINED IN THE OPERON THEY CONTROL
1,3
Cellular differentiation
The specialization of cells in both
structure and function within an organism
is the result of
1,4
Retrovirus
Which type of virus defies the “central dogma”
(DNA to RNA to Protein) of biology?
1,5
Lamarck
The theory that an organisms characteristics that develop in their
lifetime are passed on to their offspring was presented by this man
2,1
Genetic recombination
What is the single most important
factor that produces variability?
2,2
DNA is more stable.
Why has DNA become the genetic
material of cells when RNA was
considered the first form?
2,3
No gene flow, large population, random mating, no net mutations, no
natural selection.
Populations in Hardy-Weinberg
must have these 5 conditions
2,4
Allopatric Speciation
The formation of two species
due to the presence of a physical barrier
2,5
Transfer of genetic information into a cell by direct uptake of the
DNA.
What is bacterial transformation?
3,1
The restriction enzymes are used to cut DNA at specific sites
How are restriction enzymes used in genetic engineering?
3,2
Gene is found on any chromosome other than the sex
chromosome.
What is it mean to be an autosomal gene?
3,3
q=0.4 p=0.6
In a population of 10,000
1,600 are diagnosed with sickle cell
which is a recessive disorder.
What is the frequency of the two alleles?
3,4
- I
I I +
If a restriction enzyme cut a strand of
DNA into lengths of 400 bp, 2125 bp, & 300 bp
what would the corresponding gel look like?
(show starting well and +/- sides)
3,5
25%
A round watermelon is crossed
with a long watermelon,
and all of the offspring are oval.
If 2 oval watermelons are crossed,
what is the percent of
watermelons that will be round?
4,1
Only his daughters!
A man who has a sex linked allele will pass it on to…
4,2
Transcription
The process in which DNA makes messenger RNA
4,3
linkage
In flys who have yellow
bodies, they usually have mini
wings. This can best be
explained by?
4,4
24 = 64
For a species with a n=4, how
many different combinations
of chromosomes are possible
for the gamete?
4,5
Temperature, pH, salt or substrate concentration, presence of
activators/inhibitors
Name the four factors that affect the function of enzymes
5,1
DPIP serves as a substance which is reduced by the electrons
released from photosynthesis
Explain the role of DPIP in measuring the photosynthesis rate
of isolated chloroplasts in DPIP solution.
5,2
H2O
Osmosis is a special type of diffusion that allows
what molecule to move across a semi-permeable barrier?
5,3
Initial rate – highest – most substrate
End rate at 0 because substrate has been used up
Draw & Label the graph
of a reaction rate (product in moles vs. time) and
indicate the initial reaction rate &
explain why the asymptote that is approached
as the reaction finishes is present
5,4
Plants have cell plate formation where animals have cleavage
furrow
What is a major difference in during cytokinesis between
plant and animal cells?
5,5