Download Norwich_Bielski_Hulsebris_Smith_Latshaw

Experimental summary
Norwich DMSO treated versus
Control Yeast
Randy Bielski
Hans Hulsebos
Joe Smith
Melissa Latshaw
• My hypothesis is that the genes: ICL1 ,
FLR1 will be up regulated or down regulated
in the DMSO sample versus control
• I base this hypothesis on the following
information: previous research that induced
environmental stress on the Saccromycies
cerevisiae
• This information was obtained from the
following sources www.yeastgenome.org
Gene ontology
• My gene ICL1 has been assigned the following GO
categories isocitrate lyase activity (IMP, ISS),
glyoxylate cycle (TAS), cellular component
unknown (ND)
• My gene FLR1 has been assigned the following GO
categories response to toxins, multi- drug transporter
activity
Detection call summary
• Gene ICL1 had the following detection calls in the
experiment : Was present in all eight experiments and
had < .005 p value
• Gene FLR1had the following detection calls in the
experiment : Was present in all eight experiments and
had no significant p value
Fold change and p-values
• Gene ICL1 had the following F.C and p-value in DMSO
treated versus control Fold Change = 1.69; P-Value <
.005
Gene FLR1 had fold changes of 1.15; P-value was not
significant.
Were your genes in the final Set of
Selected Genes?
•
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•
ILC1 was present
FLR1 was cut from the varsity team
Gene (z)
FLR1 was not in the final analyzed set because the PValue was too high.
Making Inferences
• The following common interacting pathways exhibited
differential gene expression: serine isocitrate lysase
pathway.
Making Inferences
• Evidence for this pathway’s disregulation is further
reinforced by the following data mined from public data
bases
• Please refer to next slide… thank you
S. cerevisiae Pathway: serine-isocitrate lyase pathway
Experimental conclusion
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The gene ICL1 I anticipated to be upregulated with the treatment
of DMSO were confirmed by the data analysis and reinforced by
additional data in the public realm leading me to conclude that
ICL1 was significantly upregulated in DMSO yeast when compared
to control.
The gene FLR1 was anticipated to be disregulated with the
treatment of DMSO were not confirmed by our experimental data
–
1.
Three reasons that could have lead to the non-confirmed result
There was not a high fold change
2.
Gene might have bound with Schizosaccharomyces
pombe and was eliminated
I am now an official UVM microarray data analyst
and the results of my analysis are posted here