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tallest trees adult human chicken egg frog embryo most eukaryotic cells mitochondrion most bacteria virus proteins diameter of DNA double helix atoms Quick Review: • Where does most of the cell's work take place? Cytoplasm • What happen to the proteins once they leave the golgi apparatus and enter the cytoplasm? • become plasma membrane protein, • stay in the cytoplasm for use in the cell, • stored in vesicles until needed for transport. What is a Cell? • • • • Cell: basic or smallest living unit of life of a living organism. Protoplasm: complex jelly-like substance. 70% is water; rest is mineral salts, proteins, carbohydrates, fats. Made up of 3 main parts: (i) Cytoplasm (ii) Cell surface membrane (iii)Nucleus (i) Cytoplasm • Part of the protoplasm between cell membrane & nucleus • Jelly-like fluid in which many chemical reactions or cell activities take place. • 90% water; enzymes, organelles (specialized structures) nuclear pore chromatin (DNA) nucleus nucleolus nuclear envelope flagellum rough endoplasmic reticulum cytoplasm plasma membrane ribosome smooth endoplasmic reticulum Golgi complex free ribosome vesicle mitochondrion vesicle (ii) Cell surface membrane • Partially-permeable membrane • Controls movement of substances into and out of the cell. • Allows only some substances to cross it. (e.g. small molecules: glucose, water, O2, CO2 - prevents larger molecules from crossing: starch, proteins An electron micrograph of a cell membrane (of a human red blood cell) seen in cross-section. • made of proteins and fats • double phospholipid layers (fats, proteins, phosphate groups) (iii) Nucleus • Parts of a nucleus: - Nuclear envelope, nucleoplasm, nucleolus • Nucleoplasm: dense material inside nucleus • Nucleolus(1) or nucleoli (2): Making proteins.’ • Control cell activities e.g. cell growth, repair of worn-out parts. • Essential for cell division. nuclear envelope nucleolus nuclear pores chromatin • Chromatin: Network of long, thread-like structures • Contains hereditary material (DNA and proteins), instructions for the cell to carry out all chemical reactions • Controls cell division • Chromosomes: when chromatin threads condense and become highly coiled during cell division (rodshaped) Plant cells – Cell Wall • • • • - Non-living layer Made up of Cellulose (a complex sugar) Surrounds cell membrane of plant cell Fully permeable to water, gases, dissolved substances Protects the plant cell from injury Supports and gives a fixed shape to plant cell Membrane Network – Endoplasmic Reticulum (ER) rough endoplasmic reticulum ribosomes 0.5 micrometers smooth endoplasmic reticulum 0.5 micrometers vesicles Endoplasmic reticulum • 2 types: rough and smooth • For synthesis and transport of substances • Rough ER (RER): ribosomes are attached on outer surface • Continuous with nuclear envelope • Transports proteins made by ribosomes to Golgi Apparatus for secretion out of the cell. Endoplasmic reticulum • Smooth ER (SER): no ribosomes attached on outer surface • Makes substances – fats, steroids (sex hormones in mammals) • Detoxification – converts harmful substances into harmless materials. Ribosomes • Small round structures • Needed to make proteins in the cell • 1. Attached to RER membrane – make proteins that are transported out of the cell • 2. Free ribosomes in the cytoplasm – make proteins that are used within cytoplasm of the cell Golgi Apparatus • Stack of flattened spaces surrounded by membranes • Vesicles: tiny round structures enclosed by a membrane • Fuse on one side and pinch off from opposite side of Golgi apparatus • Stores and modifies substances made by ER • Packages the substances in vesicles for secretion out of the cell ORGANELLES MITOCHONDRION (mitochondria): • “Power house” of a cell • Aerobic respiration: food substances are oxidized to release energy • Energy is used for cell activities e.g. growth, reproduction, movement etc. PLANT CELLS - Chloroplasts • Oval shaped structures • Contain green pigment chlorophyll • Absorbs sunlight for photosynthesis, for plants to make food ORGANELLES VACUOLES • Fluid-filled space enclosed by membrane • Storage of various substances • Animal cells: many small vacuoles; store water, food. Exist temporarily • Plant cells: one large, central vacuole, filled with cell sap, contains dissolved substances e.g. sugars, mineral salts, amino acids, helps to maintain shape of plant cell • Membrane – tonoplast mitochondrion chloroplast Golgi complex central vacuole smooth endoplasmic reticulum vesicle cell wall rough endoplasmic reticulum plasma membrane nucleolus nucleus nuclear pore chromatin nuclear envelope ribosomes free ribosome ORGANELLES CENTRIOLES • Small, hollow cylinders. • Found as a pair near the nucleus. • Play an important part in cell division. • Only in animal cells. Absent in plant cells. Differences between plant and animal cells Feature Cell wall Plant cell Cell wall present Animal cell Cell wall absent No. and size of 1 large permanent vacuoles vacuole Numerous small vacuoles Relative size Large Small Chloroplast Chloroplasts present Absent Shape Regular, elongated, cylindrical Centrioles absent Irregular Centrioles Centrioles present Websites: • http://www.sciencenetlinks.com/lessons.cfm?Be nchmarkID=5&DocID=88 • http://www.beyondbooks.com/lif71/4a.asp • http://www.ljcreate.com/products/scope_demo.a sp 26