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1 Characteristics of Living Things 1. 2. 3. 4. 5. 6. 7. 8. Reproduction Has Cells Grow and Develop Acquire Energy and Materials Have DNA Respond to their Environment Maintain stable internal Environment As a group, Change over Time 2 Cell Theory • All know living things are made up of cells • Cells are the basic unit of structure and function in living things • All cells come from preexisting cells • Clip 3 Robert Hooke-1665 • One of the first people to see cells. • He saw cork Cells. • First to use the word cells- named them for the places that monks sleep in the monastery. • English scientist Robert Hooke built this microscope in the 17th century and used it to conduct pioneering research. He discovered the cell structure of plants by observing a thin slice of cork under his microscope. 4 5 Theodor Schwann 1839 Concluded that all animals are made up of cells 6 Schleiden, Matthias 1839 Concluded that all Plant cells are made up of cells 7 Cell Elemental Composition Cells are 90% water. Of the remaining molecules present, the dry weight is approximately: • 50% protein • 15% carbohydrate • 15% nucleic acid • 10% lipid • 10% miscellaneous Total approximate composition by element: • 60% H • 25% O • 12% C • 5% N 8 Two basic Types of Cells 9 Prokaryotic Cells DO NOT HAVE DO HAVE • Cell membrane • Organelles • Nucleus • Ribosomes • DNA •Are all singled celled organisms. •Thought of as more ancient life forms…they came first. •They still carry out all of life’s functions! 10 Eukaryotic Cells • Have organelles. • Can be singledcelled organisms or multicellular organisms….. Like US! 11 12 Basic Cell Structure • Cells come in many different shapes and sizes. • Like bricks in a building, cells make up all living things • Clip Basic Cell Structure The Cell Membrane • The outside of all cells are surrounded by a membrane made of phospholipids. • Nickname: “The gatekeeper” 13 14 Membrane Structure • There are proteins “stuck” in the membrane that help get things into and out of the cells. • They also help to get messages into the cell. 15 Proteins in Membrane 16 The Membrane is a lipid bilayer. 17 Plant cells also have a cell wall outside of the cell membrane. 18 • Cell walls can also be found in fungi and bacteria. • The cell wall provides support and protection for these cells. • In plants, the cell wall is made of the carbohydrate cellulose. Inside of the cell The Cytoplasm 19 The cytoplasm contains all of the organelles. • Cells are filled with organelles that each do something to keep the cell alive. • The jelly-like insides of a cell is called cytoplasm. 21 Organelles in the Cytoplasm • Each organelle has a specific function so that the cell can do its job. • Each organelle has its own job! • Remember: ONLY EUKARYOTIC CELLS HAVE ORGANELLES!!!! 22 What an organelle? • Organelle mean “tiny organ.” • Organelles function together to help the cell carry out all of life’s activities!! 24 Nucleus-The Control Center • The Brain • Contains the DNA • DNA controls which proteins get made-and when! • Where ribosomes are made 25 • The nucleus has a phospholipid bilayer around it. • The nuclear pores allow substances to move into and out of the nucleus. • The DNA NEVER leaves the nucleus. 27 Ribosomes • Not technically an organelles. • Nickname: “protein maker” • Place where proteins are made. • They help put the amino acids together to make proteins. • Made of the nucleic Acid-RNA • website 29 Endoplasmic Reticulum • Membrane system that’s function involves protein synthesis and transport. • Can be thought of as a Highway and a place of protein synthesis. 30 Two types of ER • Smooth – No attached ribosomes • Rough – Attached ribosomes 31 ER branches off from the nuclear membrane. 33 Golgi Apparatus • Nickname: “UPS” • Packages molecules and sends them to their destination. • Also checks to make sure the molecules are put together correctly, if not correct it sends them back to the ER. • Website 35 Golgi w/vacuoles Vacuoles are packages of material that are being transported. 36 Lysosomes • Nickname “Recycling Center” • Has digestive enzymes that breakdown and recycle molecules. 37 Vacuoles • Storage and transport containers. • Plants usually have one large one • Animals generally have many small ones. 38 • A vacuole is a membrane-bound sac that plays roles in intracellular digestion and the release of cellular waste products. In animal cells, vacuoles are generally small. • Vacuoles tend to be large in plant cells and play a role in turgor pressure. When a plant is well-watered, water collects in cell vacuoles producing rigidity in the plant. Without sufficient water, pressure in the vacuole is reduced and the plant wilts. 39 40 Chloroplasts Site of photosynthesis in plants. 41 Cytoskeleton • Gives cells their shape. • Contain proteins: microtubules and microfilaments. Microfilaments help give the cell shape, and movement in cytoplasm. Microtubules aids in chromosome movement, movement of organelles, and the movement of cilia and flagella. Without the cytoskeleton, the cell would have no shape. By allowing the cell to keep shape, the cell is allowed to function and stay in homeostasis. 42 Microtubules and Microfilaments 43 Cell membrane Endoplasmic reticulum Microtubule Microfilament Ribosomes Michondrion 44 Mitochondria • Nickname: “Powerhouse” • Site of ATP production • ATP is the universal energy molecule • Energy is stored in the bonds of ATP. • Website 45 Smooth endoplasmic reticulum Ribosome (free) Vacuole Chloroplast Ribosome (attached) Cell Membrane Cell wall Nuclear envelope Nucleolus Golgi apparatus Nucleus Mitochondrian Rough endoplasmic reticulum Plant Cell Organelles of the Cell Clip Nucleolus Nucleus Nuclear envelope Ribosome (attached)Ribosome (free) Cell Membrane Mitochondrian Smooth endoplasmic reticulum Rough endoplasmic reticulum Centrioles Golgi apparatus Animal Cell Animal Cell and Plant Cell Clip 48 Levels of Organization • Cells make up tissues. • Tissues make up Organs. • Organs make up organ systems. 49 How do molecules get into and out of a cell? •Movement of molecules from a place of higher concentration to a place of lower concentration •Always higher to lower! •No energy input required! Diffusion 50 51 Osmosis:Diffusion of water through a cell membrane 52 53 Transport through the membrane Two types –Facilitated Diffusion •Energy input NOT required –Active Transport •Energy input required 54 Facilitated Diffusion Energy is NOT required to move substances across the membrane. Protein 55 Facilitated Diffusion High Concentration Cell Membrane bilayer Low Concentration Glucose molecules Protein channel 55 . 56 Active Transport • Energy is required. • Need ATPthe universal energy molecule. • Low to high 57 58 Other ways to get molecules into and out of a cell Some molecules are too large to get through the membrane. Website 1. Endocytosis • Into 1. Exocytosis • Out of E N D O C Y T O S I S 59 60 61 62 Inside of the cell Exocytosis Outside of the cell solutions – The solution with the higher concentration of solutes is hypertonic. – The solution with the lower concentration of solutes is hypotonic. – These are comparative terms. • Tap water is hypertonic compared to distilled water but hypotonic when compared to sea water. – Solutions with equal solute concentrations are isotonic. 63 3 types of Solutions What types of solution? Hypertonic Or Hypotonic Plasmolysis 64 Microscopes 65 Stage Eyepiece Body Tube Nosepiece Arm 10x Objectives 40X objective 100x Objective Stage Stage Clips Coarse Adjustment Diaphragm Fine Adjustment Light Source Base • This beautiful microscope was made for the famous British scientist Robert Hooke in the late 1600s, and was one of the most elegant microscopes built during the period. Hooke illustrated the microscope in his Micrographia, one of the first detailed treatises on microscopy and imaging. Electron Microscopes • use a beam of highly energetic electrons to examine objects on a very fine scale • Co-invented by Germans, Max Knott and Ernst Ruska in 1931, Ernst Ruska was awarded half of the Nobel Prize for Physics in 1986 for his invention. • 15x to 200,000x Below are five different images of the same mosquito. • The fossilized shell of a microscopic ocean animal is magnified 392 times its actual size. The ancient creature, called Radiolarian, lived in the waters off Antarctica and is now used to study such things as climate and ocean circulation.