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Chapter 7 Cell Structure & Function Scientists & Discoveries • Early 1600’s (Holland): 1st microscope was constructed • Anton van Leeuwenhoek (1600’s) used single lens as a microscope to study and very carefully draw tiny living organisms • (1665) English physicist Robert Hooke used light microscope to study thin slices of plantssaw cork had many tiny chambers he called “cells” like monastery cells • cells= the basic units for all forms of life • (1838) Matthias Schleiden concluded all plants made of cells • (1839) Theodor Schwann animals made of cells • (1855) Rudolf Virchow -studied cell reproduction; 5yrs research concluded with: “Where a cell exists, there must have been a preexisting cell…” The Cell Theory • All living things are composed of cells • Cells are the basic units of structure and function in living things • New cells are created from existing cells. Basic Cell Structures • Cells usually measure 5-50 micrometers • Smallest: measures 0.2 micrometers • Largest: (amoeba Chaos chaos) 1000 micrometers • All cells have a cell membrane and cytoplasm • Cell membrane: thin flexible barrier around a cell • Cytoplasm: material inside the cell membrane • Many cells also have cell wall (strong layer around cell membrane) • Cell membranes & cell walls support & protect cells while allowing them to interact with their environment • Some cells also have a nucleus (a large structure that stores the cells genetic information & controls the cells activities) Prokaryotes vs. Eukaryotes • Cells are divided into 2 categories: 1. Prokaryotes 2. Eukaryotes *divided into these 2 categories to separate cells that contain a nucleus and organelles (eukaryotes) and those that do not (prokaryotes) Prokaryotes • Usually smaller & simpler than eukaryotes • Contain cell membranes & cytoplasm • DO NOT have nuclei • All bacteria are prokaryotes - examples: Escherichia coli (intestines), Staphylococcus aureus (skin infections) Prokaryotes • Carry out all activities that define life (grow, reproduce, respond to their environment, & some even move) Eukaryotes • CONTAIN nuclei, cell membrane, cytoplasm, & dozens of other specialized structures that perform special functions (organelles) • Some are single celled organisms, but most make up multicellular organisms • All plants, animals, fungi, and many microorganisms are eukaryotes Eukaryotes Section 2: Cell Structures • A. Cytoplasm- refers to the region outside the nucleus but inside the cell membrane B. Cell Membrane • Also called the plasma membrane • Bi-layer phospholipids that regulates what enters and leaves the cell C. Cell Wall *main function=provide support & protection for the cell *located outside cell membrane *found in plants and algae & nearly all eukaryotes (not animal cells) • In plants the cell wall is composed of cellulose • In fungi the cell wall contains chitin • In bacteria it contains peptidoglycan C. Nucleus *controls most of the cell processes & contains hereditary information of DNA *chromatin-granular material in the nucleus that consists of DNA bound to protein *chromosomescondensed chromatin that consists of genetic information that is to be passed on during cell division (Nucleus contd.) *Nucleolus-small , dense region where assembly of ribosomes begins *Nuclear envelopedouble-membrane layer that surrounds the nucleus. It contains many nuclear pores that allow material to move in/out of the nucleus D. Cytoskeleton *network of protein filaments that help the cell to maintain its shape, also involved in cell movement *microtubules-hollow tubes of protein, can serve as “tracks” along which organelles move *microfilaments-long, thin fibers that function in the movement & support of cells E. Organelles in the cytoplasm Ribosomes -small particles made of RNA & protein -produce proteins following coded instructions from the nucleus Endoplasmic Reticulum (ER)- organelle in which components of the cell membrane are assembled & some proteins are modified (rough E.R.-have ribosomes attached to the surface) Golgi Apparatus-enzymes in the Golgi Apparatus attach carbohydrates & lipids to proteins Lysosomes-small organelles filled with enzymes that break down lipids, carbohydrates, & proteins from food into particles that can be used by the rest of the cell (also break down organelles) Vacuoles- sac-like structures that store materials like water, salts, proteins & carbohydrates Chloroplast-found in plants -use energy from sunlight to make energy rich molecules in a process known as photosynthesis -have a double membrane Mitochondria- use energy from food to make high energy compounds that the cell can use to power growth, development, & movement -have a double membrane -found in nearly all eukaryotic cells F. Organelle DNA • Chloroplasts and mitochondria contain some of their own genetic information in the form of DNA • These small DNA molecules contain information that is essential for them to function • Could be prokaryotic cells that were engulfed by eukaryotic cells & formed a symbiotic relationship CENTRIOLES Appear during cell division to guide chromosomes apart Made up of microtubules Only seen in animal cells CENTRIOLES/MITOTIC SPINDLE Made of MICROTUBULES (Tubulin) Image from: http://www.coleharbourhigh.ednet.ns.ca/library/organelle_worksheet.htm FLAGELLA & CILIA Made of PROTEINS called MICROTUBULES (9 + 2 arrangement) Image from: http://www.stchs.org/science/courses/sbioa/metenergy/flagella.jpg FLAGELLA Help in cell movement Very few and long in length CILIA Animation from: http://web.jjay.cuny.edu/~acarpi/NSC/13-cells.htm Move cell itself And substances past the cell. Very many and short in length Section 3: Movement through the Membrane • The cell membrane regulates what enters & leaves the cell & also provides protection & support (figure 7-15) -lipid bilayer–a double layered membrane formed when certain kinds of lipids are dissolved in water -embedded proteins-forms channels & pumps that help move materials across the membrane -carbohydrate chains-attach to proteins & aids in chemical identification to allow cells to ID one another Cell Membrane Diffusion: tendency of molecules to move from an area of high concentration to an area of lower concentration to reach equilibrium (figure 7-16) -causes many substances to move across the cell membrane but does not require the cell to use energy -concentration=mass of solute/volume of solution Osmosis: diffusion of water through a selectively permeable membrane (fig 7-17) -selectively permeable= some substances can pass through but others cannot -isotonic-same strength solution on both sides -hypertonic-above strength inside (water leaves) -hypotonic-below strength inside (water enters) • Osmosis exerts pressure known as osmotic pressure on the hypertonic side of the selectively permeable membrane. -osmotic pressure can cause a cell to shrink or burst (figure 7-18) Facilitated Diffusion: the process of protein channels allowing a pathway for certain molecules to cross the membrane (figure 7-19) -100’s of different protein channels have been found that allow ions, sugars & salts to cross the membrane Active Transport: requires energy to move molecules across a cell membrane against a concentration difference. The materials move from an area of lower concentration to an area of higher concentration (figure 7-20)-often compared to a pump Endocytosis: the process of taking large particles into the cell by means of infoldings or pockets of the cell membrane that form vacuoles Phagocytosis: process of taking large particles into the cell by endocytosis. Extensions of the cytoplasm surround & engulf the large particles. Exocytosis: membrane of the vacuole surrounding material fuses with the cell membrane, forcing the contents out of the cell Review 1. What processes are made possible by the cell membrane? 2. How does diffusion affect cells? 3. Describe how water moves during osmosis. 4. What is the basic structure of a cell membrane? 5. How does active transport differ from diffusion?