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A Tour of the Cell Discovery of cells needed microscopes • 1665 – Robert Hooke observes “boxes” in cork bark; he calls them cells. • 1674 – Anton van Leeuwenhoek observes single celled organisms in well water Three types of microscope images (Figure 4.1) Cell theory 1. Schwann and Schleiden (1839) - cells are the elementary unit of all plants and animals 2. Virchow (1858)- cells come from preexisting cells 3. Modern cell theory states that cells carry out the biochemical processes of life. 4. Viruses do not fit into the cell theory and are not considered living Relative Sizes (Fig. 4.3) Cells are small • 1-100 µm (1µm = 0.001 mm) • Cells must exchange materials with their environment through their membrane. • Cells need high surface area to volume ratio in order to maximize this exchange. As size increases, surface area to volume ratio decreases Length SA = 6 * length2 V = Length3 SA/V 1 6 1 6 2 24 8 3 3 54 27 2 4 96 64 1.5 5 150 125 1.2 Two types of cells: Prokaryotes and Eukaryotes Prokaryotes are bacterial cells • Archaea, Eubacteria – Most primitive organisms • No inner structure • DNA floats in cytoplasm • External structures – Cell wall – Flagella - move – Pili – for attachment and transfer DNA Bacteria Fig. 4.4 Eukaryotes • All other kingdoms – Animalia – Plantae – Fungi – Protist kingdom(s) Animal Cell (Fig. 4.5) Plant Cell (Fig. 4.5) External Eukaryote Structure Cell membrane • Isolates cell from outside environment • Regulates movement of molecules in and out of cell. • Permeable to small molecules and non-polar molecules; impermeable to polar molecules and ions. Three main components of cell membrane • Phospholipid bi-layer: two layers of phospholipids situated with hydrophilic ends facing out ward and hydrophobic tails facing inward • Cholesterols – Four ringed lipids, regulates the fluidity of membrane • Proteins – Hydrophobic a.a within membrane with hydrophilic a.a outside Fluid Mosaic: molecules in membrane move freely (Fig. 4.6) Three Types of Membrane Proteins • Transport proteins: allow specific molecules to enter/exit cell • Receptor proteins: bind to molecules ( i.e. hormones, nutrients) • Recognition proteins: cell specific proteins that identify cell. Flagellum and Cilia • • • • Used for locomotion, moving particles Made of protein filaments Cilia – many “hairs” Flagellum – Usually a single undulating “tail” Flagellum and Cilia (Fig. 4.22) Inside a Eukaryote • • • • • Cytoplasm Organelles made of phospholipid bilayer Nucleus containing chromosomes Mitochondria – in most eukaryotes Chloroplast – in plants and some protists Cytoplasm • • • • Viscous liquid Dissolved molecules Organelles High concentration of proteins Nucleus 4.8 • Contains chromosomes which stay inside nucleus • Nucleolus - site of ribosome production Ribosomes (Fig. 4.10) • Small protein subunits (Large and small) • Site of protein synthesis Mitochondria (Fig. 4.20) • Site of Kreb cycle and ATP production • Inner folds increase surface area Chloroplasts Fig. 4.19 • Contain chlorophyll (green pigment) • Site of photosynthesis Endoplasmic Reticulum • Membrane extension of the nucleus • Many folds = lots surface area • Rough ER – contain ribosomes which make proteins • Smooth ER – makes phospholipids • Makes new membrane • Break down toxins Endoplasmic Reticulum (Fig. 4.13) Assembly of a protein Golgi Complex (Fig. 4.15) • Protein modification • Protein storage Vacuoles (Fig. 4.17) • Membrane bound storage structures • Contractile vacuoles regulate water content Lysosomes (Fig 4.16) • Membrane bound enzymes • Digest food from extracellular fluid or damaged organelles Cytoskeleton provides structure and support (Fig. 4.21) • Interconnected proteins • Can change arrangement and location within a cell (Fig. 4.21)