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Chapter 13 Name In order to determine the genotype of a particular 1 trait of an organism, researchers can perform a test cross between the unknown organism and an organism that is known to be . A homozygous dominant for the trait B heterozygous for the trait C homozygous recessive for the trait D a clone 2 How can foreign pieces of DNA be transferred into organisms? A by restriction enzymes B by vectors C by carrier enzymes D by test crosses Date process is a common practice that has 5 Which been used by farmers for hundreds of years to develop new varieties of plants and animals? A cloning B genetic engineering C cutting DNA and removing segments D selective breeding for desirable traits The diagram below shows some key steps of a 6 biotechnological procedure. What development does the letter X most likely represent? Enzymes + Gene for human insulin Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc. 3 The model below represents the outcome from a step used in the recombinant DNA process. What is the step called? Class Cut bacterial DNA Inserted into bacterial cell Cell division X A B C D human cells able to resist antibiotics human cells unable to synthesize antibodies bacterial cells able to synthesize human insulin bacterial cells unable to synthesize human insulin The nucleus is removed from a body cell of one 7 organism and is placed in an egg cell that has A B C D DNA sequencing gel electrophoresis cloning genes splicing genes had its nucleus removed. This process results in the production of organisms that are genetically alike. What is this process called? A fertilization B adaptation C cloning D inbreeding 8 Restriction enzymes are used for what purpose? Researchers inserted a gene for bioluminescence 4 into a variety of organisms, such as mosquito larvae. What is this technology called? A gel electrophoresis B polymerase chain reactions C DNA sequencing D genetic engineering STANDARDIZED TEST PRACTICE A to maintain homeostasis B to transfer DNA fragments to plasmids C to cleave DNA strands at specific nucleotide sequences D to inhibit enzyme reactions in cells CHAPTER 13 25 Chapter 13 Name was encapsulated in a virus like the one 9 DNA shown below and supplied to a patient for the purpose of replacing a defective gene. What is this treatment called? Date Class DNA fragment has been cleaved by bm Arestriction enzymes, as shown in the model below. After this occurs, each single strand is left with what feature? GATAT C C TATAG A B C D sticky ends blunt ends spliced genes a complete genome are the small circular, double-stranded bn What DNA molecules in bacteria that can be used as gene therapy pharmacogenomics gene splicing replication variety of strawberry is resistant to a bk One damaging fungus but produces small fruit. Another strawberry variety produces large fruit but is not resistant to the fungus. How might the desirable qualities of the two varieties be combined? A cloning B asexual reproduction C direct harvesting D selective breeding has a gene that causes a visual disorder. bl ATowoman prevent the disorder from appearing in future generations, where would the defective gene have to be repaired? A nervous system B reproductive cells C eye D uterus 26 CHAPTER 13 vectors? A clones B plasmids C chloroplasts D transgenic organisms can a polymerase chain reaction be bo Why compared to a biological copy machine? A It makes a single, perfect copy of a DNA sequence. B It identifies a DNA sequence. C It is used to make millions of copies of a specific DNA molecule. D It delivers gene therapy to all the cells of the body. Dolly is a sheep developed from an egg of her bp mother that had its nucleus replaced by a nucleus from a body cell of her mother. What resulted from using this technique? A Dolly is no longer able to reproduce. B Dolly is genetically identical to her mother. C Dolly, or others like her, have a longer than normal life span. D Dolly is unable to mate. STANDARDIZED TEST PRACTICE Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc. A B C D