Download Chang Liver (HeLa derivative) (cervix, Human)

Cat. No. EC88021102
Chang Liver (HeLa derivative)
(cervix, Human)
Culture Medium :
EMEM(EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids(NEAA) + 10% Fetal Bovine
Serum (FBS)
Cell Line description :
The cell line Chang Liver was originally thought to be derived from normal liver. The cell line has
since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, The cell
line should be considered as derived from HeLa.
DESCRIPTION OF REPOSITORY REFERENCE SEED STOCK
Morphology : Epithelial.
Sub Culture Routine : Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4
×10,000 cells/cm 2 using 0.25% trypsin /EDTA; 5% CO 2; 37℃.
Karyotype : Modal no. 70.
Depositor : Obtained from ATCC.
Originator : No.
Country : USA.
References : Nat Cancer Inst Monogr 1962; 7: 249.
Proc Soc Exp Biol Med 1954; 87: 440.
Nature 1976; 259: 211.