Download Chromosome Spread of HeLa Cancer Cells CellServ Kit 4

Chromosome Spread of HeLa Cancer Cells
CellServ Kit 4: Preparation of Human Chromosome Spreads
Objective: Describe the morphological differences between normal and
cancerous cells, and relate those differences to the cell cycle.
(The term “morphology” refers to the form and structure of an organism or a cell)
Day 1 Procedures:
1. Read pages 1 and 2 of the provided CellServ Kit 4 documents (Introduction,
Teacher Preparation, and Objectives).
2. Read the provided CellServ Kit 4 “Student Procedures”.
3. This block, you must complete steps 1-9 to prepare your slides.
4. While your slides are air drying (Step 8), refer to the provided Background
Information to answer the following questions in your Biochemistry Journal.
a) Compare and contrast: diploid cells and aneuploid cells. Explain each term
and evaluate how they are alike/different.
b) Explain HOW you determine whether a cell is diploid or aneuploid.
c) Predict: What total chromosome number you expect to observe for your
prepared cells. Why?
5. During our next block, we will complete Steps 10-11 and observe your slides.
Morphology of Normal versus Cancer Cells
CellServ Kit 1: Visualization of Normal & Transformed Cells
Objective: Describe the morphological differences between normal and
cancerous cells, and relate those differences to the cell cycle.
DAY 2 Procedures:
1. Re-read the provided Cell Serv Kit 4 “Student Procedures”.
2. Complete Steps 10-11 to observe your prepared cells using the 100X oil
immersion objective as demonstrated by Dr. Goldin.
3. RECORD your observations. Your observations must include
a) The NAME of the cell type you are observing.
b) The TOTAL MAGNIFICATION at which you observed your cell(s).
c) A DRAWING of the cell(s).
d) At least FOUR other details of your observation.
4. And for the kicker…. COUNT and RECORD the TOTAL CHROMOSOME NUMBER
for at least 6 different individual cells on your slide.
5. Using your iPads and other research materials available to you, answer the
“Student Assessment” questions in your Biochemistry Journal.
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Student Assessment
1. Describe: What are “HeLa cells”? Provide a detailed explanation.
2. Are HeLa cells diploid or aneuploid? Support your response with YOUR
OWN DATA obtained from your observations.
3. Explain WHY HeLa cells contain the observed number of total chromosomes.
(In other words: HOW did they get like that?)
4. Relate your response to 3 above to the cell cycle.
(In other words: What happened or didn’t happen during the cell cycle that
caused HeLa cells to get like that?)