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Chapter 3: Cell Structure and Function MRS. WILLIAMS FRESHMAN BIOLOGY SEMESTER ONE Robert Hooke Mid-1600s: First to describe cells Used a compound microscope Two lenses Actually looking at dead cork cells Anton van Leeuwenhoek Made many microscopes (single lens) Describes several types of cells First to study living, moving organisms (from pond water) with microscope Fig. 3.2 – page 71 Cell Theory The cell theory is a fundamental concept of biology! It states: All living things are composed of cells. Cells are the basic units of structure and function in living things. New cells are produced from existing cells. Compound Microscope Staph bacteria under a compound microscope Electron Microscope Transmission (TEM) Specimen cut into very thin slices Beam of electrons pass through Scanning (SEM) Samples are dehydrated, put in a vacuum, and sometimes coated in materials like gold Electrons are bounced off the surface Produces 3-D images of the surface Electron Microscope Staph bacteria under an electron microscope Other EM Images Clockwise from top left: spider, bacteria, microorganism (probably bacteria) Classifying Cells – RA Activity Read the page 72 in your textbook. Construct and color code a Double Bubble Map comparing and contrasting prokaryotic and eukaryotic cells. Be prepared to share with your partner, and then with the class! Classifying Cells Prokaryotes Lack organized structures Circular loop of nucleic acid No nucleus Examples: bacteria and blue-green algae Eukaryotes Organized structures called organelles DNA in nucleus Examples: animal, plant, fungi, and protists "Cells: Basic Units of Life" Video Eukaryotic Cells Three regions Cell membrane Nucleus Cytoplasm Cell Membrane Cell Membrane Function Regulates what comes in and out of cell Selective permeability – read on page 83! Communication Protection and Support Phospholipid bilayer Double layer of phospholipids Fluid Mosaic – read about on page 82! Phospholipid molecules with other molecules (proteins and carbohydrates) embedded The membrane is in constant movement Phospholipids - Revisited Polar Head Hydrophilic Attracted to water Non-polar Tail Hydrophobic Doesn’t want to be near the water Membrane Proteins Allow larger molecules to pass through the membrane Can regulate what comes in or out (is the cell’s bodyguard!) Other Molecules Cholesterol Stabilizes the membrane Keeps non-polar tails from sticking to each other Carbohydrate chains Identification markers Cell Wall Plants only Rigid outer layer covering the cell membrane Allow plants to support heavy structures like flowers Contains cellulose and various proteins Movement Across Membranes Passive Transport Diffusion (Simple and Facilitated) Osmosis Active Transport Protein Pump Endocytosis and Exocytosis Movement is controlled by concentrations Concentration Amount of solute (dissolved substance) in a volume of solution Expressed as mass/volume Amount of mass is proportional to the concentration Volume is inversely proportional to the concentration Passive Transport Movement from an area of high concentration to an area of low concentration (down the concentration gradient) Requires NO energy Examples: Diffusion (simple and facilitated) and Osmosis Diffusion Movement of solute from high to low concentrations Requires no energy Continues until equilibrium is reached Facilitated Diffusion Solute cannot simply cross cell membrane because it is semi-permeable Solute movement is aided by membrane proteins Allows diffusion of molecules that are too large to diffuse through the membrane using simple diffusion Osmosis Movement of water from an area of high concentration to low concentration Requires no energy Solutions surrounding cells can be…(read on page 86!) Hypertonic – solution has high [solute]* compared to the inside of the cell Isotonic – solution has the same [solute] as the inside of the cell Hypotonic – solution has a low [solute] compared to the inside of the cell *[solute] = solute concentration Active Transport Movement of solute from an area of low concentration to high concentration (across or up the concentration gradient) Requires energy (using ATP) Examples: Protein pumps, endocytosis, and exocytosis Protein Pumps Membrane protein pumps solute across cell membrane Solute moving from low to high concentration Requires ATP energy Endocytosis and Exocytosis BOTH: Movement of large materials across the cell membrane Read and take your own notes about each type – page 90 Endocytosis Movement into cell Pocket of membrane pinches off to form vesicle (membrane circle surrounding material) Two types Phagocytosis – solid material Pinocytosis – dissolved material Exocytosis Movement out of cell Phagocytosis and Pinocytosis Exocytosis Cytoplasm The cytoplasm includes everything INSIDE the cell membrane except the nucleus Also includes the fluid cytosol Where do Organelles Come From? Thought to originally be prokaryotes that formed a symbiotic relationship with What is symbiosis?! another cell Called the Endosymbiotic Theory Evidence Many organelles are surrounded by two membranes Some organelles contain their own DNA Nucleus Control center of the cell Double membrane with many pores Contains DNA Chromatin Chromosomes Nucleolus Small, dark region Makes ribosomes Ribosomes Site of protein synthesis Link amino acids together to form proteins Two subunits made of RNA and protein Found free floating in cytoplasm or attached to rough ER Endoplasmic Reticulum Series of membrane bound canals Two Types Rough Studded w/ ribosomes Produces and transports proteins Smooth No ribosomes Produces and transports lipids Golgi Apparatus Stack of flattened pancake-like membranes Modifies, packages, and ships out lipids and proteins Lysosome Vesicle filled w/ digestive enzymes Breaks down cellular debris Aids in programmed cell death (apoptosis) Vacuole Animals Many small membrane bound sacs Storage compartments for water, food molecules, inorganic ions, and enzymes Plants Usually one large central sac Used for storage of water, toxins, waste products, and pigments that give color (like in flower petals) Water storage helps establish turgor pressure to keep plant upright Mitochondria Double membrane Inner membrane highly folded Power house of cell (makes ATP’s) Only inherited from mother Contains DNA Chloroplast Only in Plants Double membrane Converts sunlight energy into chemical energy Contains chlorophyll pigment Captures light energy Contains DNA Cytoskeleton Skeleton for Cell Helps cell maintain shape Provides support and protection Aids in movement Made of microtubules and microfilaments Cilia Short, hair-like microtubule extensions Move in oar-like motion Move cell or move materials on the surface of cells Cells usually have many Flagella Long, whip-like microtubule extension Move in whip-like fashion Moves cells Cells usually only have one or a few Centrioles Only in animal cells Grouped microtubules Aids in cell division Moves chromosomes with spindle fibers Animal Vs. Plant Cells Organelle Animal Plant Nucleus YES YES Cytoplasm YES YES Cell membrane YES YES Cell wall NO YES Lysosome YES YES Ribosome YES YES ER YES YES Mitochondria YES YES Animal Vs. Plant Cell Continued Organelle Animal Plant Golgi YES YES DNA YES YES Vacuole YES (small, several, only in a few animal cells) YES (large, single) Cytoskeleton YES YES Chloroplast NO YES Centriole YES NO Flagella YES (some) NO Cilia YES (some) NO Cell Thinking Map! Make a brace map of all the parts of the cell and how they fit together. Skill: Whole to Part Relationship Unicellular Organisms Organism made of a single cell Very simple One cell performs all the functions of life Ex: Bacteria, protists, some fungi Multicellular Organisms Organisms made of many cells More complex Cells specialize and perform certain functions (cell differentiation) All cells work together to perform all the functions of life Ex: animals, plants, and fungi Levels of Organization Levels of Organization Atom Molecule Macromolecule Cell Tissue- cells working together towards a common goal Organ- tissue working together towards a common goal Organ system- organ working together towards a common goal Organism