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A Tour of the Cell • Anton Van Leeuwenhoek (1600’s) • Credit for the first microscope • Looked at pond water and saw “wee beasties” Robert Hooke • Observed plant stems, wood, and cork (1600’s) • Saw all the tiny chambers and called them CELLS • What cell part did Hooke observe? • Cell Wall Robert Brown (1833) • Observed that cells had a dark structure within plant cells • Brown observed the nucleus and stated that all cells have nuclei (at this time no one knew that the nucleus has DNA) Matthias Schleiden (1838) • Stated that all plants are made of Cells • Made many observations of plants around the area Theodor Schwann (1839) • Stated that all animals are made of Cells • Observed many animal tissues Rudolf Virchow (1855) • Stated that all cells come from pre-existing cells • Cells arise from the division of pre-existing cells The Cell Theory Microscopes Provide the Windows to the World of the Cell The Cell Theory • All living things are composed of cells • Cells are the basic unit of structure and function in living things • All cells come from pre-existing cells A Prokaryotic Cell Figure 7.1 The size range of cells The Size Range of Cells Cell Sizes Average Animal Cell – 15 microns Average Plant Cell – 40 microns Average Eukaryotic Cell :10-100 microns Average Prokaryotic Cell: 1-10 microns An Electron Microscope Geometric Relationships Explain Why Most Cells Are Microscopic Overview of an Animal Cell Human Cheek Cells Overview of a Plant Cell Onion Epithelial Cells Animal Cell’s Cell Membrane Cell or Plasma Membrane •“Fluid Mosaic” Model • Lipid Bilayer (made of phospholipids) •Proteins embedded throughout •Semi-permeable or Selectively Permeable Cell Wall • provides support to the perimeter of plant cells, some protists, and bacterial cells The Plasma Membrane The Nucleus and Its Envelope Nuclear Envelope/Membrane •Double Membrane that surrounds the nucleus •Lined with pores •Supported by nuclear lamina Nucleolus •Inside the nucleus •Site of ribosome and rRNA synthesis Ribosomes Endoplasmic Reticulum (ER) Endoplasmic Reticulum (ER) •Rough ER •Intercellular transport of materials, particularly proteins; site where proteins leave ribosomes and are chemically modified Smooth ER • breaks down toxic substances, • regulates Ca levels, • synthesizes steroids and other lipids The Golgi Apparatus Golgi Apparatus •Modifies proteins and other substances from the ER for export from the cell Lysosomes Lysosomes •Digest cellular waste and foreign substances •Breakdown of lipids, carbohydrates, and proteins The Formation and Functions of Lysosomes • Relationships among organelles of the endomembrane system 1 Nuclear envelope is connected to rough ER, which is also continuous with smooth ER Nucleus Rough ER 2 3 Membranes and proteins produced by the ER flow in the form of transport vesicles to the Golgi Smooth ER cis Golgi Nuclear envelop Golgi pinches off transport Vesicles and other vesicles that give rise to lysosomes and Vacuoles Plasma membrane trans Golgi Figure 6.16 4 Lysosome available for fusion with another vesicle for digestion 5 Transport vesicle carries 6 Plasma membrane expands proteins to plasma membrane for secretion by fusion of vesicles; proteins are secreted from cell Peroxisomes •Contain an assortment of enzymes that perform such roles as detoxification of alcohol, breaking down of fatty acids •Produces H2O2 in the process Peroxisomes The Chloroplast, Site of Photosynthesis Plastids •May be called chromoplasts or leukoplasts •Store starch, fat or contain pigments such as chlorophyll or carotenoids to capture energy from the sun The Mitochondrion Mitochondrion Site of cellular respiration and synthesis of ATP, a source of chemical energy for the cell The Plant Cell Vacuole Vacuoles •Store water, salts, proteins, carbohydrates, or enzymes The Cytoskeleton Cytoskeleton •Protein strands that give the cell its shape and size •Helps organize the location of organelles and their activities There are three main types of fibers the make up the cytoskeleton: 1) Microtubules 2) Microfilaments 3) Intermediate Filaments Microtubules •Are made of the protein tubulin •Shape and support the cell •Are responsible for the separation of chromosomes during cell division Centrosome Containing a Pair of Centrioles Centrioles •Appear during mitosis in animal cells; are composed of nine sets of triplet microtubules in a ring Centrosome •Area from which the centrioles radiate during mitosis Figure 7.24 Ultrastructure of a eukaryotic flagellum or cilium Ultrastructure of a Cilium or flagellum Cilia and Flagella •In eukaryotes, a specialized arrangement (“9 + 2”) of microtubules is responsible for the beating of flagella and cilia •The protein, dynein, is responsible for the movement How Dynein “Walking Moves Cilia and Flagella A Comparison of the Beating of Flagella and Cilia The microtubule assembly of a cilium or flagellum is anchored in the cell by a Basal Body, which is structurally identical to a centriole. Sea Urchin Sperm Microfilaments and Motility Microfilaments also aid in •Cell motility (Ex: pseudopodia) •Cell division (cleavage furrow formation) •Cytoplasmic Streaming A Structural Role of Microfilaments Microfilaments •Made of the protein actin •Located in the cytoplasm of most eukaryotic cells •Works with myosin to cause muscle cell contractions Intermediate Filaments •Anchor nucleus and other organelles •Reinforces cell shape •Make up nuclear lamina that lines the interior of the nuclear envelope Plant Cell Walls Intercellular Junctions in Animal Tissues Extracellular matrix (ECM) 1)What are the two major types of electron microscopes? 2) All cells are classified as either _______ or _____. 3) Under the microscope, bacteria are typically measured in ___________ (units). 4) Two similarities between plant and animal cells are. . .. two differences are . . .. 5) The term used to describe the fact that the cell membrane allows some materials in and keeps others out is. . . 6) The lipid bilayer of the cell membrane is made of: 1)The organelle that packages proteins for export from the cell is the . . . 2) Cellular respiration occurs in the ___________ and energy is made in the form of ________. 3) The major difference between a prokaryotic and eukaryotic cell is that a prokaryotic cell lacks a/an: 4) The oldest cells on earth are ______ cells. They evolved . . . . years ago. What characteristic of the cell membrane allows some molecules into the cell and keeps other out? The primary purpose of the cell wall is.. The cell membrane is composed primarily of. . Why is the cell membrane referred to as a “fluid mosaic model”? Since some molecules can pass through the cell membrane and others cannot it is termed. . If a molecule is too big to get through the cell membrane, it must enter through _________ channels. The centers of protein synthesis in the cell are the _________. Describe the role of the cytoskeleton in the cell. Name two different things stored by vacuoles.