Download Cabbage（Brassica oleracea L

Title: Engineering Brassica with insect-resistance by stacked gene of
trypsin inhibitor（sporamin）and chitinase genes
M.S. student: Chen Pengjen
Advisor: Kai-Wun Yeh
Abstract:
Cabbage（Brassica oleracea L. Capitata）is the most important leafly vegetable
in Taiwan, due to it’s abundant antioxidant compounds and anticancer compounds like
glucosinolate. Unfortunately, cabbage can be easily infected with or attacked by insect
peats. In order to decrease the risk of cabbage attacked by insects, the goal to create
transgenic cabbage by gene stacking methods is inprogress in our lab. Trypsin
inhibitor（sporamin）and chitinase genes were in stack constructed using pMSPOA as
promoter,. We successfully got ten independent lines through Agrobacterium
tumefaciens-mediated transformation,. In trypsin inhibitor or chitinase activity assay,
we found that both sporamin and chitinase activity can be increasing 1-2 times at 2
hrs after wounding. In bioassay, we found that transgenic line 4 is more effectivel to
defend Diamond-back moth(Plutella xylostella) attack..
In order to alleviate public concerns on the risk of antibiotics resistance gene,
recommendations have been made to eliminate all antibiotic marker genes from GM
plants. Our results showed that by Agrobacterium tumefaciens-mediated
co-transformation with a double T-DNA binary vector in Arabidopsis, several
marker-free transformants were successfully obtained. This technology mote will
further applied in the transgenic cabbage system.