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Course Code : GBE 214 Level : Undergraduate Status : Compulsory Instructor : COURSE DESCRIPTION COURSE OBJECTIVES COURSE CONTENTS TEACHING/ASSESSMENT Course Title : TECNIQUES IN MOLECULAR BIOLOGY Year : II Semester : IV Hours/Week : 5 ECTS Credits : 5 Total Hours : 75 The course covers the definitions and applications of biotechnology and genetic engineering, describe the correct way for experiments design, sampling, collection of results, their analysis and interpretation, error detection and correction. The techniques presented in this course would provide students with an excellent resume for future career opportunities in biotechnology. The laboratory portion of the course is structured to integrate the goals of a selected specific research project with the training, such that the generated results will be meaningful in the real world.. This provides the necessary tools for advanced graduate thesis research utilizing modern genetic approaches in the sciences. General experimental procedures Laboratory safety General operating procedures Emergency contact information Getting oriented; practicing with micropipettes Isolation of plant DNA Isolation of RNA Quantitative Estimation of DNA and RNA Southern blot analysis of plant DNA Rapid isolation of plasmid DNA Miniprep of plasmid DNA Restriction digestion of plasmid DNA Agarose DNA electrophoresis of the digested plasmid DNA Preparation of bacterial cloning vectors Preparation of insert DNA Preparation of transformation-competent cells DNA ligation and transformation of E.coli Screening transformants Polymerase chain reaction (PCR) Protein extraction from plant tissues Protein quantification SDS-PAGE protein electrophoresis Coomassie staining of proteins and gel documentation Description 1. Interactive lectures and communications with Teaching Methods students 2. Tutorials Description(%) Student Assessment Methods Quizzes 10% Homework 10% Project 20% Midterm Examination 20% Final Examination 40% Learning Outcomes Actively participate in courses and begin to take responsibility for learning Begin to work effectively as part of a team, developing interpersonal, organisational and problem-solving skills within a managed environment, exercising some personal responsibility. Present information in oral, written or graphic forms in order to communicate effectively with peers and tutors. Language of Instruction English Textbook(s) 1) The Condensed Protocols. From Molecular Cloning: A Laboratory Manual (2006). Sambrook, J and Russell, D. W., CSHL PRESS