Download GBE 214 TECNIQUES IN MOLECULAR BIOLOGY

Course Code : GBE 214
Level : Undergraduate
Status : Compulsory
Instructor :
COURSE DESCRIPTION
COURSE OBJECTIVES
COURSE CONTENTS
TEACHING/ASSESSMENT
Course Title : TECNIQUES IN MOLECULAR BIOLOGY
Year : II
Semester : IV
Hours/Week : 5
ECTS Credits : 5
Total Hours : 75
The course covers the definitions and applications of biotechnology and genetic engineering,
describe the correct way for experiments design, sampling, collection of results, their analysis
and interpretation, error detection and correction.
The techniques presented in this course would provide students with an excellent resume for
future career opportunities in biotechnology. The laboratory portion of the course is structured to
integrate the goals of a selected specific research project with the training, such that the
generated results will be meaningful in the real world.. This provides the necessary tools for
advanced graduate thesis research utilizing modern genetic approaches in the sciences.
 General experimental procedures
 Laboratory safety
 General operating procedures
 Emergency contact information
 Getting oriented; practicing with micropipettes
 Isolation of plant DNA
 Isolation of RNA
 Quantitative Estimation of DNA and RNA
 Southern blot analysis of plant DNA
 Rapid isolation of plasmid DNA
 Miniprep of plasmid DNA
 Restriction digestion of plasmid DNA
 Agarose DNA electrophoresis of the digested plasmid DNA
 Preparation of bacterial cloning vectors
 Preparation of insert DNA
 Preparation of transformation-competent cells
 DNA ligation and transformation of E.coli
 Screening transformants
 Polymerase chain reaction (PCR)
 Protein extraction from plant tissues
 Protein quantification
 SDS-PAGE protein electrophoresis
 Coomassie staining of proteins and gel documentation
Description
1. Interactive lectures and communications with
Teaching Methods
students
2. Tutorials
Description(%)
Student Assessment Methods Quizzes
10%
Homework
10%
Project
20%
Midterm Examination
20%
Final Examination
40%
Learning Outcomes
 Actively participate in courses and begin to take responsibility for learning
 Begin to work effectively as part of a team, developing interpersonal, organisational
and problem-solving skills within a managed environment, exercising some personal
responsibility.
 Present information in oral, written or graphic forms in order to communicate
effectively with peers and tutors.
Language of Instruction
English
Textbook(s)
1) The Condensed Protocols. From Molecular Cloning: A Laboratory Manual
(2006). Sambrook, J and Russell, D. W., CSHL PRESS