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BIOL 212 SI Exam 2 Review 10/10/13 Ch. 6: Energy, Enzymes, & Metabolism 1. What is true about the following graph? a. It is exergonic & will occur spontaneously b. It is exergonic & will not occur spontaneously c. It is endergonic & will occur spontaneously d. It is endergonic & will not occur spontaneously 2. You are performing an experiment on enzymes & inhibitors; you time the enzyme without the inhibitor and notice it takes 8 seconds for the reaction to go to completion. You then time the enzyme with an inhibitor and notice it takes 20 seconds to go to completion. What kind of inhibitor are you testing? a. Competitive b. Noncompetitive 3. +3.3kcal/mol -7.3kcal/mol If the above reactions are coupled they will have a net ΔG of: a. +4kcal/mol endergonic b. -4kcal/mol exergonic c. +4kcal/mol exergonic d. -4kcal/mol endergonic 4. T/F competitive inhibitors bind the active site of an enzyme while noncompetitive inhibitors bind an allosteric site Ch. 7.1-7.2: Respiration 5. If cyanide is added to a cell, will NADH and FADH2 be oxidized in the electron transport chain? a. NADH will but FADH2 won’t b. NADH won’t but FADH2 will c. Both will because cyanide blocs complex IV cytochrome oxidase, so everything will work normally up until here, O2 will not be consumed & ATP will not be produced d. Neither will 6. What products of pyruvate breakdown are used in the citric acid cycle? a. ATP b. CO2 c. Acetyl d. NADH 7. Which step of glucose breakdown produces the most ATP? a. Glycolysis b. Pyruvate breakdown c. Citric Acid Cycle d. Oxidative Phosphorylation 8. Which method of ATP production do cancer cells (hypoxic – lack oxygen) prefer? a. Glycolysis b. Oxidative Phosphorylation Ch. 8: Photosynthesis 9. T/F the Calvin Cycle converts CO2 to O2 The Calvin Cycle incorporates CO2 into carbohydrates and the light reactions produce O2 10. T/F In the light reactions the high energy electron transfers from pigment molecule to pigment molecule until it reaches the special pigment molecule called P680. The energy transfers, the electron itself does not transfer. 11. What product of the light reactions is used by the Calvin Cycle? a. O2 b. ATP c. NADPH d. a & b e. b & c f. All of the above 12. What happens to the electron as it moves from PSII to PSI? a. Gains energy b. Loses energy, this is why a 2nd input of light is needed at PSI to boost it back up to high reducing potential c. Energy stays the same **Review SI worksheet from 9/26/13 for more on photosynthesis!** Ch. 11: Nucleic Acid Structure, DNA Replication, & Chromosome Structure 13. In Griffith’s experiment, a mixture of heat killed S strains and live R strains of bacteria killed mice, why was this so? a. R strains are lethal b. Heat killed S strains are lethal c. There was contamination & some of the S strains were live, they needed to add DNase, RNase, and protease to clean it up d. A biochemical genetic material from the heat killed S-strains transformed the live R strains into live S strains 14. If protein was the genetic material, what would have happened in the Hershey & Chase experiment? a. The infected cells would have shown 35S radioactive label b. The infected cells would have shown 32P radioactive label c. The infected cells would not have been radioactive at all 15. What will be the newly synthesized strand if 5’-ATCCGAGTT-3’ is the template for DNA replication? a. 5’-TAGGCTCAA-3’ b. 5’-AACTCGGAT-3’ c. 3’-ATCCGAGTT-5’ d. 3’-UAGGCUCAA-5’ 16. Which enzyme is responsible for relieving tension brought on by supercoiling ahead of the replication fork? a. Helicase b. Topoisomerase c. Primase d. Polymerase e. Ligase Ch. 12: Gene Expression at the Molecular Level 17. If this is the DNA coding strand, what is the mRNA transcript? 3’-TAGGCATT-5’ a. 5’-AUCCGUAA-3’ b. 3’-AUCCGUAA-5’ c. 3’-UAGGCAUU-5’ d. 5’-UAGGCAUU-3’ 18. T/F the promoter region of a gene is transcribed 19. T/F One gene encodes one protein 20. The start codon binds a tRNA carrying the amino acid methionine, what amino acid charged tRNA does a stop codon bind? a. Methionine b. Arginine c. Cysteine d. None of the above, a stop codon doesn’t bind a charged tRNA, it binds a release factor **For more on translation study figures 12.18, 12.19, 12.20, and 12.21 in your book** Ch. 13: Gene Regulation 21. Most gene regulation occurs at what level? (In both eukaryotes & prokaryotes) a. Transcriptional b. RNA Processing c. Translational d. Post-Translational 22. Which of the following is true? a. Enhancers bind activators and stimulate transcription b. Activators bind enhancers and stimulate translation c. Activators bind enhancers and stimulate transcription d. Repressors bind silencers and stimulate transcription 23. What does the promoter region of a eukaryotic gene consist of? a. Regulatory elements, TATA box, transcriptional start site b. TATA box, transcriptional start site c. Regulatory elements, TATA box 24. Which of the following is true? a. miRNAs cause mRNA degradation b. siRNAs inhibit mRNA translation c. miRNAs inhibit mRNA translation d. Both miRNAs and siRNAs cause mRNA degradation Ch. 9: Cell Communication 25. What protein converts ATP to cAMP? a. PKA b. Phosphorylase Kinase c. GPCRs d. Adenylyl cyclase 26. Which of the following are examples of 2nd messengers? a. cAMP b. Ca2+ c. DAG d. IP3 e. a &b f. All of the above 27. Which of the following are possible responses to epinephrine (aka adrenaline)? a. Glycogen breakdown b. Glycogen synthesis inhibited c. Apoptosis d. a & b e. All of the above 28. T/F phosphorylation of a protein always activates that protein Ch. 36: Flowering Plant Behavior 29. What does gibberellin do? a. Release trapped transcription factors b. Induce phototropism c. Stimulate cell division and elongation d. a & b e. a & c f. All of the above