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The Essentials of Life Science Research Globally Delivered™ Test your nucleic acid knowledge! Across 1 1. DNA is often resuspended in molecular-grade water or _______. 4. Chemical compound comprising a nitrogencontaining base, one or more phosphate groups, and a five-carbon sugar. 7. Reading frame without a stop codon. 4 N U C 10. The most abundantant intracellular RNA is ______ RNA. 16. Monocyclic base 9 P C T R A 11 17. Bicyclic base Down 2. Method used to evaluate the purity of a nucleic acid preparation. 3. In prokaryotes, transcription and ________ are coupled. 5. DNA is made up of the following organic bases: adenine, _____, guanine, and cytosine. 6. The secondary structure of DNA refers to the _______ bonding between base pairs. 8. The right-handed helix is the favored confirmation, and is termed the _________. 9. Method for evaluating the functional activity of DNA. 5 7 11. Process where RNA is synthesized from DNA. 14. Nucleic acid research took a great leap forward when Watson and Crick proposed the __________ structure of DNA in 1953. 2 T R I S P L E O T I D E H C Y T M O R I O N P E H O N S C R I P T E O Q M O U B L E H E T E P Y R N C Y I I N E G 14 D 12 16 17 P U R 3 E D T A R A N S H B Y L F D A H E R T R I B O S O M A L G O I E X I O N V N I R L I X U S M I M I D I N E S L K 6 8 10 13 15 12. Method used to confirm species identity. 13. Microorganisms that can have RNA as their genomic material. 15. For filamentous fungi, adding _____ powder solution to a PCR reaction may enhance PCR efficiency. (Hint: See the ATCC Genuine Nucleics guide) p 800.638.6597 703.365.2700 e [email protected] [email protected] www.atcc.org © 2013 American Type Culture Collection. The ATCC trademark and trade name, any and all ATCC catalog numbers and any other trademarks listed in this publication are trademarks of the American Type Culture Collection unless indicated otherwise. ATCC® the essentials of life science research. Globally delivered.™ 10801 University Boulevard, Manassas, VA 20110