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Biology 321 Spring 2013 Answers to Assignment Set #6 Answers to text questions in 10th edition: Chapter 7 http://fire.biol.wwu.edu/trent/trent/IGA_10e_SM_Chapter_07.pdf Chapter 8 http://fire.biol.wwu.edu/trent/trent/IGA_10e_SM_Chapter_08.pdf Chapter 9 http://fire.biol.wwu.edu/trent/trent/IGA_10e_SM_Chapter_09.pdf Chapter 16 http://fire.biol.wwu.edu/trent/trent/IGA_10e_SM_Chapter_16.pdf Answers to text questions in 9h edition: Chapter 7 http://fire.biol.wwu.edu/trent/trent/CH07.169-174_10625.pdf Chapter 8 http://fire.biol.wwu.edu/trent/trent/CH08.175-180_10625.pdf Chapter 9 http://fire.biol.wwu.edu/trent/trent/CH09.181-188_10625.pdf Chapter16 http://fire.biol.wwu.edu/trent/trent/CH16.229-256_10625.pdf Answers to Biology 205 review questions: 1. peptide bonds 2. tertiary 3A. isoleucine & phenylalanine 3B. 4 & 5 4. The tertiary structure of a globular protein is determined in part by the hydrophobic effect – nonpolar amino acid side chains are sequestered in the interior of a protein. Polar side chains will typically be on the surface of the protein (interacting with water). This mutation has resulted in the substitution of an amino acid with a polar side chain for an aa with a non-polar side chain and might very well affect the stability and/or formation of the proper tertiary structure. 5. & 6. single-stranded DNA 5’ carbon is at the top of the chain – with 3 phosphates; 3’ carbon is at the bottom with the free hydroxyl 7. 9. c a. 8. a (note though that nucleotide sequences are typically written 5’ to3’) 10. c 11. b & c 12. c 14: e. 15: a. nucleoside = nucleotide without a phosphate b. 1. carbonyl 2. pyrimidine 3. hydroxyl 4. modified deoxyribose 16. DNA consists of two polymers; adenine base-pairs with thymine and guanine with cytosine 17. the top pyrimidine 18. Watson drew guanine in its rare tautomeric form which (due to its pattern of H-bond donor and acceptors) H-bonds with thymine not cytosine 1 1 19. 5’ GUU 3’ anticodon will base-pair with a 5’ AAC 3’ codon which specifies asparagine (ASN) 20. promoter 21. a&b. see problem 20 c. specific sequences which are typically high in A’s and T’s (TATA box) 22. 5 amino acids: mRNA will have the same sequence as the top strand (excluding the promoter which is not transcribed) with U’s replacing T’s. Translation begins at the first AUG and ends with the first in-frame stop codon: UAA 23. DNA is the permanent archive of the genetic database; RNAs typically have a short half-life. Errors made during DNA synthesis in a multicellular organism (that remain unrepaired) are passed onto the next generation of cells (if somatic) or progeny (if in the germline) 24. The codon is 5’ UAC 3’ and will specify tyrosine 25. See text description of mRNA, tRNA and rRNA 26. b 27. d. 28. See lecture notes and text 29. T T F 30. a. ase means the protein has catalytic activity b. transcript in the standard context means an RNA molecule that is generated from a DNA template C. the retrovirus infection cycle involves generating DNA molecules using RNA as a template – the reverse of what usually happens in the cell during transcription 31. Answer is a. The mRNA reads 5’ CGUAAUCAUG…. etc. (antiparallel to the template). Translation starts with the first AUG and ends with the first in-frame stop codon: UAG Answers to Additional Problems Problem -2 Problem -1 5’ promoter 3’ intron exon 2 2 d. transcription e. The exons and intron sizes are not to scale – introns would be much longer than indicated here. f. The exon sequence represented in black is translated and the exon sequence in white is not translated. The translation initiation codon (AUG) and a translation termination codon (UAG in this case) define the translation boundaries. Problem 0 [3 X 109 base pairs/genome copy] X [1 X 10-8 mutations/ base pair] X 2 genome copies = 60 mutations (60 base pair changes between parents and child) Problem 1 The mutation rate per gamete in Drosophila represents mutations that have accumulated after a series of mitoses that gave rise to the germline in the embryo and to the cells that eventually under go meiosis. So more DNA replication events are represented by the per gamete rate. Problem 2 The 3’-5’ exonuclease activity associated with DNA polymerases corrects most mistakes that occur during the DNA chain elonation process. DNA mismatch repair systems correct errors that remain post-DNA synthesis. RNA polymerases do not have proofreading capabilities and there are no RNA repair systems. Why does this make sense in the context of the different roles of DNA and RNA in the cell? Problem 3 Answer: c Problem 4 a. See lecture notes for labelling of the carbons. b. dideoxy means that there is no hydroxyl group on either the 2’ or 3’ carbon. c. This compound must be converted into the triphosphate form (added to the 5’ carbon) before it can serve as a substrate for viral DNA polymerase d. The TP version of dideoxyI can be added to the 3’ end of a DNA chain, but DNA replication terminates because there is no 3’ hydroxyl -- which is required for addition of the next nucleotide. Problem 5A a. After a couple of rounds of replication: CG to TA transition b. Thymine replaces uracil in DNA so that the cell can recognize the cytosine deamination product (uracil) as abnormal and initiate a repair process. If uracil were used as adenine’s pairing partner in DNA, there would be a problem differentiating “normal” uracils that should be in the DNA from uracils that are a cytosine decay product. NOTE: I think I remember reading that a G:T repair system was just discovered that changes the T to a C in a GT mismatch. This is not a postreplication mismatch repair system which could change the G to an A OR the T to C depending on which strand was the parental. Problem 5B a. thymine b. a GC to AT transition will result 3 3 Problem 6 Answer is c. A G C G* C C T A rep G C ___ ____ = apurinic site = the phosphodiester backbone (phosphate –ribosephosphate) is still intact but the ribose doesn’t have a base attached to it Problem 7 a. Some spontaneous mutations are the result of mistakes made during DNA replication. Furthermore, many (all of the examples discusssed in class) spontaneous and induced mutations require rounds of replication to establish the error in both strands of DNA. Therefore the more a cell divides, the greater the risk of mutation. Paternal gametes undergo many more rounds of replication than maternal gametes. Since the germline cells divide continually in a male, in an older father, the germ cells will have undergone more round of replication. b. NOTE: the mutation (GGU AGU) is designated in codon jargon, which, by convention, is in RNA language. BUT, we are not talking about a transcription error -- which obviously does not meet the definition of a mutation. diagram on the next page 4 4 5 5 Problem 10 MUTANT #1 a. nonsense b. tyr stop UAU/C --› UAA/G each case is a transversion c. a base modifying agent might, depending on which bases it chemically modified and whether an apurinic/apyrimidinic base resulted. MUTANT #2 a. frameshift b. trp UGG =› one of the G's is deleted c. intercalating agent MUTANT #3 a. missense b. tyr UAU --› CAU or UAC --› CAC both are transition mutations c. base analog or base modifying agent d. mutant #3 Problem 11 (i) e (b & c) Mutant 1 could result from (i) UAu/c --> UAG or UAA transversion , (ii) from a deletion of the 3rd base in the tyr codon which would generate a UAA stop codon or (iii) from an insertion of a G or an A after the UA of the tyr codon resulting in a UAA or a UAG stop codon (ii) e (a & b) (iii) a Problem 12 a. FH380: missense FH 763: frameshift FH 683: nonsense b. UAU/C tyrosine UGU/G cysteine A to G transition c. mRNA 5’….GUG UAC CAA…. DNA 5’ …. GTG TAC CAA….3’ 3’ ….CAC ATG GTT… 5’ bottom strand serves as the template for mRNA synthesis 5’ ---A--- 3’ 3’ ---T--- 5’ ----G --- --- G------ T* ------ C --- T* = thymine in rare tautomeric form = round of DNA replication (drawing follows one parental strand only) d. I’m going to testify for the defense for the following reasons 1. The father and mother need to be assessed. It is likely that one or the other is heterozygous for the same mutation their son has. Do either have a history of heart attacks? Have their genotypes been determined? 6 6 2. Even if the son results from a new mutation it couldn’t have been caused by the father’s exposure to hydroxylamine. The son’s mutation is a AT to GC transition. Hydroxylamine cannot cause this mutation because it only modifies cytosines and would not act at an AT base pair. 3. Defense probably pays more (just kidding) e. Note ARG is specified by 6 different codons. • The mutation could be CGn UAG or AGa/g UAG • The first alternative CGn UAG requires transition mutations in the first two base pairs of the codon (assuming that n=G). Neither aflatoxin or proflavin will accomplish this. The second alternative AGa/g UAG is a possibility. Inserting a T before this codon would result in a UAG and, as an intercalating agent, proflavin could cause this type of mutation. Since both parents are likely to be homozygous wild-type, I’ll go with the prosecutors on this one. Problem 18 a. Neutral Sequence variation does not affect the Darwinian fitness of the organism. b. Yes. The alternative sequence variation exceeds 1% c. These sequence variations are not disease causing. You would want to focus your screen on the mutations in Table 2. d. It is in the third codon position so likely to be a same sense or silent mutation e. It is in the first codon position so likely toe be a neutral missense Problem 19 1. Red arrows point to exons. Why are some exons white, some black or half and half? Green arrows point to introns. Translational start and stop codons 2. Truncating mutations: 7 7 NONSENSE premature terminaton of translation due to mutations that converts a codong specifying an amino acid into one of the three STOP codons FRAMESHIFT an indel that is not a multiple of 3 results in a shift of reading frame. Reading frame is established by the AUG that serves as the intiation codon. In the alternative reading frames there will typically just by chance be stop codons that will cause a shorted polypeptide to be produced: ORF= open reading frame = a DNA sequence that does not contain a stop codon in a given reading frame[ SPLICE SITE MUTATION: incorrect splicing of primary transcript could lead, for example, to a mRNA with an intron not properly removed (or exon skipping – or other possibilities). An intron mistakenly included in the mRNA may result in a shift in reading frame that affects the translation of all exon sequence downstream (3’) of it and, like frameshifts generated by indels, will result in premature termination of translation due to a STOP codon in the alternative reading frame. NON-truncating mutations: MISSENSE: substituting one amino acid for another so no effect on reading frame or translational stop codon IN-FRAME INDEL insertion or deletion of a multiple of 3 bases results in the addition or deletion of amino acids but no shift in frame 3. The initiator AUG codon sets the reading frame. If it is mutated and therefore unreconizable by the translational machinery, it (the ribosome) may choose the next best alternative AUG. So a mutation in the initiator AUG may lead to a truncated polypeptide if the wrong reading frame is chosen by the ribosome. Problem 20 a. Review definition of polymorphism in earlier lecture b. silent or same sense mutation c. Neutral missense mutation (note legend at bottom of table that indicates that all people genotyped were healthy non-NIDDM) d. Examination of a normal control group is important because some sequence variations will be associated with disease and others will have no obvious effect on the encoded protein or on the organismal phenotype. In order to understand how the gene product functions and to assess the phenotypic implications of a particular genotype, it is critical that researchers be able to distinguish between these two types of sequence variations. e. Intron sequences are non-coding – not translated into protein 8 8 Problem 21 GAU/C (asp) CAU/C transversion GC CG Start here: Have G* pair with G in first round of replication, etc 5’ GAT 3’ 3’ CTA 5’ Problem 22 Answer is C Problem 23 a. Gain-of-function and loss-of-function mutations can have opposite phenotypes. b. Class 1 mutations are loss-of-function mutations: they are recessive and more common than Class 2 mutations. Furthermore, one class1 mutation is known to affect splicing -- which is consistent with a loss of gene function. Class 2 represents a gain-of-function mutation. NOTE this problem illustrates an important point: whether a mutation causes a loss or gain-of-gene function reflects the effect on the gene product’s normal function NOT whether there has been an increase or decrease in a physiological process. c. The normal gene product inhibits or lowers production of thyroid hormone. Problem 24 See cancer genetics lecture. Addition of wild-type copies of a tumor suppressor gene or a guardian of the genome might correct the mutant phenotype. But it wouldn’t help in the case of an oncogene (mutant proto-oncogene). 9 9