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Cellular Transport Bynum-Biology Tuesday, September 8, 2015 Objective: SWBAT… Explain how the process of diffusion, passive transport, and active transport occur and why they are important to cells. Predict the effect of a hypotonic, hypertonic, or isotonic solution on a cell. Essential Questions: 1. Can you distinguish the difference between osmosis and diffusion with respect to cellular function and ATP for removal of material? 2. Can you compare and contrast the two types of cellular transport? Vocab you should know! Osmosis isotonic hypertonic hypotonic passive transport facilitated diffusion active transport endocytosis exocytosis Warm-up & Daily Writing Tuesday, September 8, 2015 Writing Assignment Excitement that never ends: Describe the last thing you got really excited about – the butterflies in the stomach, giggling, can’t wait excited. 1. 2. 3. 4. 5. 6. Warm-Up In order for a cell to survive, what substances might need to get into a cell or out of a cell? Do you think it would be easier for you to get to a place by walking with the wind or against the wind? Why? Draw and label a phospholipid. What is the most important characteristic of the cell membrane? What is diffusion? What is osmosis? Homeostasis in a Cell Remember: Homeostasis is maintaining a stable internal balance. The cell membrane is structured with phospholipids that have proteins embedded in them to help regulate homeostasis in a cell. The cell membrane is a semi-permeable (allowing certain things to enter and exit the cell) barrier that surround the cells. The cell membrane is the cell organelle in ALL cells that regulate homeostasis for the cell. Osmosis: Diffusion of Water Recall: Diffusion is the movement of particles from an area of higher concentration to an area of lower concentration. In a cell, water ALWAYS tries to reach an equal concentration on both sides of the membrane. The diffusion of water across a selectively permeable membrane is called osmosis. Regulating the water flow through a plasma membrane is an important factor in maintaining homeostasis within the cell. Osmosis: Diffusion of Water If you add sugar to water the water becomes sweeter. The number of sugar molecules will increase and the number of water molecules will decrease. If two solutions of high sugar molecules and lower sugar molecules are separated by a selectively permeable membrane that only allows water to diffuse across, water flows to the side of the membrane where the water concentration is lower. During osmosis, water diffuses across a selectively permeable membrane. Notice that the number of sugar molecules did not change on each side, but the number of water molecules did. Osmosis: Diffusion of Water The water will continue to diffuse until it is at equal concentration on both sides of the membrane. Therefore, we know that the unequal distribution of particles (concentration gradient) is one factor that controls osmosis. Cells in an isotonic solution Most cells (unicellular and multicellular) are subject to osmosis because they are surrounded by water solutions. In an Isotonic solution, the concentration of dissolved substances in the solution is the same as the concentration of dissolved substances inside the cell. The concentration of water is the same both inside the solution and inside the cell. Cells that are in isotonic solutions do not experience osmosis and they maintain normal shape. Cells in an Hypotonic solution In an Hypotonic solution, the concentration of dissolved substances is lower in the solution outside the cell than the concentration inside the cell. Therefore, there is more water outside the cell than inside. Cells in a hypotonic solution experience osmosis that causes water to move through the plasma membrane to the inside of the cell. This causes the cell to swell and its internal pressure increases. In animal cells If the solution is extremely hypotonic the plasma membrane maybe unable to withstand the pressure and can burst. Because plant cells contain a cell wall, they do not burst in a hypotonic solution. Where does this occur at in daily life? Cells in an Hypertonic solution In an Hypertonic solution, the concentration of dissolved substances outside the cell is higher than the concentration inside the cell. Meaning, the concentration of water is higher on the inside of the cell. Cells in hypertonic solution experience osmosis that causes water to flow out. In animal cells, hypertonic solutions shrivel because of the decreased pressure in the cells. (This is why you should not salt meat – it will cause the water to leave the meat leaving it dry & tough) Plant cells, in hypertonic environment mainly loose water from the central vacuole. The plasma membrane will shrink away from the cell wall. The loss of water will result in a drop of pressure – explaining why plants wilt. Passive Transport Water and lipids are some of the substances that can pass through the membrane via diffusion. The cell uses no energy to move these particles. Therefore, this movement of these particles by diffusion is called passive transport – because it requires no energy to move the particles. Particles that move via passive transport are moving WITH the gradient. Proteins can move substances through the membrane via transport proteins. These proteins function in variety of ways to transport molecules. The passive transport of materials across the membrane with the aid of transport proteins is called facilitated diffusion. Active Transport A cell can move particles from a region of lower concentration to a region of higher concentration, but it will require energy to counteract the force of diffusion that is moving from the opposite direction. The movement of particles against the concentration gradient (from low concentration to high concentration) is called active transport and it requires energy from the cell. Some cells can take in large molecules, groups of molecules, or even whole cells. Endocytosis, is a process by which a cell surrounds and takes in material from its environment. This material does not pass through the membrane instead it is engulfed and enclosed by a portion of the membrane. That portion of the membrane breaks away and the resulting vacuole with its contents move inside the cell. Exocytosis, is the expulsion or secretion of materials from a cell. Cells use exocytosis to expel waste and secrete substances, such as hormones produced by a cell. Concept Check On a separate piece of paper – answer the following questions for class work 1. What factors affect the diffusion of water through a membrane by osmosis? 2. How do animal cells and plant cells react differently to osmosis in hypotonic and hypertonic solutions. 3. Compare and contrast active and passive transport. 4. Draw a cell that is in a isotonic, hypertonic, and hypotonic solution. Draw what the cell is like before and after diffusion. Be sure to include labels for the direction of the flow of water. 5. Compare and contrast endocytosis and exocytosis. Why would a cell use either of these processes?