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Download Deoxyribonucleic acid from calf thymus (D4522)
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Deoxyribonucleic acid, Activated from calf thymus Product Number D 4522 Storage Temperature –20 °C # CAS 91080-16-9 Synonym: Activated calf thymus DNA Product Description The Activated Calf Thymus DNA is prepared by modification of a published method using calf thymus DNA (Product No. D 1501) and DNase I (Product No. 1 D 4263). It is suitable as a substrate for DNA polymerase. The product is lyophilized from a DNA solution containing 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA. Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Preparation Instructions The lyophilized DNA should be reconstituted overnight at 2–8 °C to insure that all the material goes into solution. When the product is fully dissolved, the DNA concentration can be determined by measuring the absorbance at 260 nanometers (A 260) and using the following formula: µg/ml of DNA = A260 x 50 µg/ml x DF A260 = absorbance of the DNA solution at 260 nm 50 µg/ml = the concentration of 1 A260 unit of dsDNA DF = the dilution factor (typically a 100-fold dilution with reconstitution buffer for a 1 mg/ml solution) Storage/Stability It is recommended to store the product at –20 °C. References 1. Aposhian, H.V., and Kornberg, A., J. Biol. Chem., 237, 519 (1962). EWK,MAM 04/05-1 Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip.