Download Deoxyribonucleic acid from calf thymus (D4522)

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Transcript
Deoxyribonucleic acid, Activated
from calf thymus
Product Number D 4522
Storage Temperature –20 °C
#
CAS 91080-16-9
Synonym: Activated calf thymus DNA
Product Description
The Activated Calf Thymus DNA is prepared by
modification of a published method using calf thymus
DNA (Product No. D 1501) and DNase I (Product No.
1
D 4263). It is suitable as a substrate for DNA
polymerase.
The product is lyophilized from a DNA solution
containing 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and
1 mM EDTA.
Precautions and Disclaimer
This product is for R&D use only, not for drug,
household, or other uses. Please consult the Material
Safety Data Sheet for information regarding hazards
and safe handling practices.
Preparation Instructions
The lyophilized DNA should be reconstituted overnight
at 2–8 °C to insure that all the material goes into
solution. When the product is fully dissolved, the DNA
concentration can be determined by measuring the
absorbance at 260 nanometers (A 260) and using the
following formula:
µg/ml of DNA = A260 x 50 µg/ml x DF
A260 = absorbance of the DNA solution at 260 nm
50 µg/ml = the concentration of 1 A260 unit of dsDNA
DF = the dilution factor (typically a 100-fold dilution with
reconstitution buffer for a 1 mg/ml solution)
Storage/Stability
It is recommended to store the product at –20 °C.
References
1. Aposhian, H.V., and Kornberg, A., J. Biol. Chem.,
237, 519 (1962).
EWK,MAM 04/05-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser
must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of
the invoice or packing slip.