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1 Cell Unit Part A: What are cells A brick house is made of many bricks. A brick is the basic unit of structure of a brick house. The basic unit of structure in living things is a cell. All living things are made up of one or more cells. It is also the basic unit of function. Cells carry out all life processes. For example, a cell takes in food and breaks the food down. It breaks down a simple sugar called glucose to produce energy. This life process is called cellular respiration. The first person to observe cells was Robert Hooke. Hooke was an English scientist. He used a compound microscope to look at a think slice of cork. A compound microscope is an instrument used for looking at very tiny objects. It has more than one lens so it can magnify more than a simple magnifying glass. Cork is actually the bark of a certain type of tree. The cork Hooke saw seemed to be made up of many small boxes. You can see what Hooke saw when he looked in the microscope in the picture to the left. Each box looked like a small room with walls around it. The boxes reminded Hooke of the rooms monks slept in called cells. Hooke named the structures that made up cork “cells”. When Hooke looked at the cork, he did not actually see living cells. He saw the remains of living cells. Another scientist was able to see actual living cells for the first time. His name was Anton van Leeuwenhoek. Anton was a Dutch lens maker who had lots of time on his hands. In 1675, he saw single celled organisms in a drop of pond water. These living things were microscopic. They could not be seen without a microscope. As microscopes got better, we gained more knowledge about cells. By 1800, better microscopes were being made. Many plants and animals were studied. In the mid-1800’s, two guys worked to find out more about cells. They were Schleiden who studied plant cells and Schwann who studied animal cells. Their ideas were put together as a theory. A theory is an idea that explains something. The ideas in a theory are supported by data over and over. The theory that was developed was the cell theory. It has 3 parts. They are: 1. All living things are made up of one or more cells. 2. Cells are the basic units of structure in living things and they carry on all life processes. 2 3. Cells come from other living cells. By the 1930’s, a new type of microscope was invented. It used a beam of electrons passed through a thin object to make an image. It was called an electron microscope. Light microscopes can magnify things hundreds of times. Electron microscopes can magnify things tens of thousands of times. Now we could really see all the tiny parts of the cell. There are two types of electron microscopes, a transmission electron microscope (TEM) and a scanning electron microscope (SEM). The SEM can only look at the surface of an object. The TEM looks at the insides. In either case, you can only look at dead things because the object must be in a chamber without any air. Also with a TEM, you must have a very thin slice of what you want to look at. It must be about 1/100th of the thickness of a hair on your head. The picture to the right shows the ebola virus. Without a very high powered EM, you would not be able to see the virus. It was magnified 160,000 times. 3 Name:_______________________ Date:________________________ Part A: What are Cells Review 1. What is a cell? 2. Who gave cells their name? 3. Who was the first person to see living cells? 4. What is a theory? 5. What are the three parts of the cell theory? a. b. c. 6. What is the relationship between improved microscopes and discoveries about cells? 7. A light microscope passes a beam of light through an object to show an image. How does an electron microscope show an image? 8. Which two guys came up with the cell theory? 4 Part B The compound light microscope The first compound microscope was invented about 1590 by two Dutch lens makers, Hans and Zacharias Janssen. Many scientists made and used them. Much of what is known about living things would not be possible with the microscope. All compound microscopes have the same basic parts. Using a microscope can be a lot of fun. It is easy to use if you know its parts and what they do. As you read about each part of the compound microscope, find the part in the drawing. • Eyepiece – The eyepiece is located at the top of the microscope. It holds the ocular lens. • Body – the body is a hollow tube that light passes through. It holds the lenses apart. • Nosepiece – The nosepiece holds the objective lenses. • Objective – There are several objectives on your microscopes. They are attached to the rotating nosepiece. Each lens has a different magnification. The smallest objective will magnify the least. You will always begin looking at a slide using the lowest power objective. • Neck – the neck supports the body tube, and is used to carry the microscope. • Coarse-focus knob – This knob turns and is used to raise and lower the stage to focus the microscope. You only need to use the coarse adjust with the low power objective. • Fine-focus knob - This knob also raises or lowers the stage. It does not move the stage as much. You should use the fine-adjust when 5 • • • • • you are on medium or high power. This knob brings the object into sharp focus. Stage – the stage is a place where the object you are looking at is put. Stage clips – the stage clips hold down the slide on the stage. Diaphragm – The diaphragm changes the amount of light entering the objective. Light source – the light source is located under the diaphragm. It sends light toward the hole in the stage. The light source for our microscopes is a light bulb. Base – The base is the bottom part of the microscope. You should carry the microscope by on hand holding the neck and one hand under the base. To figure out how much an image is magnified you would multiply the magnification of the eyepiece lens with the magnification of the objective lens. Total magnification = eyepiece x objective The magnification of the eyepiece is always 10x for our microscopes. The magnification of the objective lens is 4x for lowest power, 10 x for the medium power and 40 x for the highest power objective. So if you are looking through the highest power objective, the object would be magnified 10 x 40 or 400 times. 6 Name:___________________ Part B The Compound Light Microscope Review 1. Who invented the first compound microscope? 2. How do you carry a microscope properly? Write the word or words that best complete the following sentences. 3. The lens at the top of the microscope is found in the ____________________________________________ . 4. When looking through a microscope the ___________________________________ lens is closest to your eye. 5. The nose piece holds the __________________________________________. Our microscopes have three, low power, medium power and high power. 6. The coarse focus knob should only be used the _____________________________________ objective. 7. The object to be viewed is placed on the __________________________________ of the microscope. 8. The amount of light entering the microscope is controlled by the ________________________________________. 9. The highest power objective by itself magnifies _____________________times. 10. The longest objective that is attached to the nosepiece magnifies the ________________________________________ . Fill in the total magnification for the combinations of eyepiece lenses and objective lenses table below 11. 12. 13. 14. 15. Eyepiece mag. 5x 5x 10 x 10 x 10 x Objective mag. 10 x 40 x 4x 10 x 50 x Total Magnification 7 Part C What are the main cell parts? There are three main parts. The three main parts of cells are the cell membrane, the nucleus and the cytoplasm. These three main parts are shown in the picture to the left. There are other parts of cells in the picture but we will talk about them later. The nucleus of a cell is round or egg-shaped. It is usually near the middle of the cell. The nucleus is usually darker than the rest of the cell. It is the control center of the cell. It controls all the life processes of a cell. The nucleus also controls cell reproduction. The nucleus is made up of DNA which carries all the info to build every substance you make in your body. The DNA is kept in something called chromosomes. You can only see chromosomes when the cell is dividing. The cell membrane is a thin structure that surrounds the cell. Sometime the cell membrane is called the plasma membrane. The cell membrane has three important jobs. They are : 1. It protects the inside of the cell. 2. It supports the cell 3. It controls the movement of materials into and out of a cell. Food, water, and oxygen must move through the membrane into the cell. Wastes move out of the cell through the membrane. A diagram of a cell membrane can be seen to the right. It is made up of two layers of molecules. One end of the molecule loves water and one end hates water. The end that hate water line up facing each other with the water loving ends facing into the cell where there is a lot of water and outside the cell where there is lots of water. There are 8 also lots of what look like globs and antennae in the membrane. The globs are special hallways and the antennae are markers that all cells have. The third main part of a cell is called the cytoplasm. The cytoplasm is all the jelly like substance that is inside the cell membrane but not in the nucleus. All the little structures in the cell float in the cytoplasm. Most of the cell is made up of cytoplasm. Most of the cells activities take place in the cytoplasm. The cytoplasm also helps to slowly move substances around the cell. 9 Name:__________________________ Part C What are the main cell parts? Review 1. What are the three main parts of a cell? a. b. c. 2. What would happen to a cell if the nucleus were taken out? 3. What is one thing the cytoplasm does? 4. What are three jobs of the cell (plasma) membrane? a. b. c. Fill in the blanks with the word or words that best complete the sentences. 5. The ________________________________________controls all the life processes of a cell. 6. The cell membrane is also called the _____________________________________ membrane. 7. The cell membrane controls the _____________________________________ of materials into and out of the cell. 8. Most of the cell is made up of _______________________________________. 9. The cell membrane is made up of ___________________________ layers of molecules. 10. One end of the cell membrane molecules ___________________________ water and line up facing each other. The other end _______________________________ water and lines up facing the inside of the cell and the outside of the cell. 11. The nucleus of a cell also is surrounded by a membrane. What do you think one of the jobs of the nuclear membrane is? 10 Part D : Two Types of Cells All cells have a cell membrane. All cells have cytoplasm. Not all cells have a nucleus. I can tell you are shocked. Yes, it is true. Some cells have a nucleus and some do not. Because of this, all cells can be divided into whether they have nuclei or not. The two types of cells are prokaryotic and eukaryotic. Prokaryotic cells do not have a nucleus. They are usually very simple and very, very tiny. Instead of a nucleus prokaryotic cells have some DNA inside the cell. It doesn’t have a membrane around it. It is just floating there. All bacteria are prokaryotes. The picture to the right shows a typical bacterium. Eukaryotes are organisms that have a nucleus in their cells. They are more complex and are usually larger in size. Not only do eukaryotes have a nucleus with a membrane around it they have other organelles (little cell organs) with membranes around them. Your organs do specific jobs for you. Your heart moves your blood around your body. Your brain controls your body parts. Organelles are tiny structures in cells that do something for the cell. They break down food, they move stuff around, they store stuff. You are a eukaryote. As a matter of fact all organisms except bacteria are eukaryotic. The picture to the left shows a typical eukaryotic cell. You can see there is definitely more “stuff” inside the eukaryotic cell. 11 Part E: What are other parts of a cell? You can compare a cell to a factory. There are many machines in a factory. Each machine has a special job. The machines work together to keep the factory working. The “machines” of the cell are its organelles. Organelles are small structures that float in the cytoplasm. Each organelle has a special job to do. They keep the cell working properly. One kind of organelle is the mitochondria. Mitochondria are small, rice-shaped structures. In fact, they are so small they can only be seen with an electron microscope. Mitochondria are powerhouses of the cell. They break down food to make energy for the cell. The energy is used by the cell to carry out its life processes. You can see a diagram of a mitochondrion to the left. Every cell has many small round structures in its cytoplasm. These structures are called ribosomes. Ribosomes are little protein factories. A cell needs protein for growth and these little organelles make it. They are pictured to the right. Once the ribosomes make the protein sometimes it has to be changed or packaged for storage. The Golgi apparatus does this. The golgi apparatus looks like a bunch of zip lock bags stacked on top of each other. Its kind of like the gift wrap department in Macy’s or J.C. Penny. You give the gift wrapper your present and they give it back nicely wrapped. The Golgi gets the protein and sends it out nicely wrapped. The Golgi is shown in the picture to the left. After the substances are packaged, they need to be moved around the cell. We have already talked about how cytoplasm moves things very slowly but there needs to be a quick transport system. This is done by the endoplasmic reticulum (ER). The ER is a network of connecting tubes that are usually found by the nucleus. It moves things really fast in the cell. Its also a place ribosomes like to hook on. You can see the ER to the right. 12 Sometimes substances are not needed right away. In that case they have to be stored. The storage containers for a cell are called vacuoles. Vacuoles come in all sizes and store lots of different things. They are kind of like Gladware plastic containers for the cell. Vacuoles in plant cells are very large because they hold water that keeps the cell’s shape. Plant cells usually have one big vacuole. Animals cells have lots of little vacuoles. A large vacuole can be seen in the plant cell to the left. Sometimes the cell will make special substances called enzymes that are used to break down things or kill invaders. The cell needs to keep its enzymes in a vacuole. These special enzyme vacuoles are called lysosomes. They are the “suicide sack” of the cell. When the cell is worn out, it opens its lysosomes. This breaks the cell up so it can be recycled. There is actually an organelle inside the nucleus. It is called the nucleolus. The nucleolus usually shows up as a dark spot in the nucleus. It’s job is to make ribosomes. The ribosomes leave the nucleus and go out into the cytoplasm to make protein. You can see a nucleolus in the picture of the nucleus to the right. Another organelle found in eukaryotic cells is a cytoskeleton. The cytoskeleton is a network of protein fibers that give the cell support and shape. In a cell, it acts a great deal like your skeleton does in you. An EM picture of a cells cytoskeleton is seen to the left. Some cells need an organelle to move themselves around. This is done in one of two ways. One way cells move is with a long whip-like tail called a flagellum. You are probably seen a picture of a cell with flagella in health class. Sperm have flagella. Other cells move around with lots of tiny hair-like structure that stick through the membrane. These beat in rhythm like oars on a Viking ship. They are called cilia. 13 14 15 Name:_____________________________ Date:_______________________________ Part E: What are other parts of a cell? Review Match the organelle in the left column with its function in the right column. Please write your answer in the space in the left margin. If you would like to change your answer, cross it out and write your final answer next to it. Tiny round organelle that makes protein. ______1. Cell membrane Sometimes they are attached to the ER. “control center “ for the cell. It has DNA in it and ______2. cilia is in charge of all the workings of the cell Small hair-like structures that stick through the cell ______3. cytoplasm membrane and beat like oars on a boat Storage container of the cell. Plants usually have ______4. cytoskeleton one big one filled with water, animal cells have lots of little one “suicide sack” of the cell made of a vacuole with ______5. Endoplasmic reticulum enzymes in it that break down invaders or used up cell parts. Powerhouse of the cell that breaks down food to ______6. flagella make energy for the cell Thin structure that surrounds the cell and controls ______7. Golgi apparatus what can go into the cell and what can leave Network of tubes that move “stuff” around the ______8. lysosome cell very quickly Network of fibers made of protein that give the ______9. mitochondria cell shape and support Single whip-like structure that cells use to move ______10. nucleolus around. Stack of flattened sacks that package the stuff ______11. nucleus cells make Only organelle found in the nucleus. It makes ______12. ribosome ribosomes Jelly-like liquid found inside the cell that moves ______13. vacuole things very slowly 16 Part F How do plant cells and animal cells differ? All plant cells have a cell wall. Animal cells do not have a cell wall. The cell wall surrounds the cell membrane. The cell wall is nonliving. It is made up of a hard material called cellulose. Wood is made up of mostly cellulose. Bacteria and fungi cells also have cell walls but they are made of something different. The cell wall has three jobs. It protects a plant cell and gives the cell its shape. It also gives a plant cell support. Large plants, such as trees and bushes, do not need a skeleton because each cell has support from the cell wall. The number and size of vacuoles is different in plant and animal cells. Plant cells have only one or two vacuoles. The vacuoles as you read in the last section are usually very large. Animal cells have many small vacuoles. Most plant cells have chloroplasts. Chloroplasts are round green structures. They contain a green material called chlorophyll. Chlorophyll gives a plant its green color. Chlorophyll is very important to plant cells. Plants need chlorophyll to make food. Animal cells do not have chloroplasts or chlorophyll. Animal cells do have one organelle that plant cells do not. It is called the centrioles. Centrioles hang out together in a pair near the nucleus. When the cell is getting ready to divide, the centrioles go to opposite sides of the nucleus. Centrioles play some part in guiding the chromosomes to opposite sides of the cell during cell division. Plant cells do not have centrioles. 17 Name:_________________________ Date:__________________________ Part F Cell Organelle Review 1. What is the job of the centrioles in a cell? 2. Which organelle has the job of making food for plants? 3. What are the three jobs of the cell wall? a. b. c. 4. How are the vacuoles in animal cells different from the vacuoles in plant cells? 5. Name the three types of organisms that have cell walls around their cells. a. b. c. 6. Label the following organelles in the diagrams of the plant and animal cells on the next pages. Remember some organelles are only in plants, some only in animals and most are in both. Cell membrane Mitochondria Lysosome (bonus) Centrioles Ribosome Cell wall Chloroplast Golgi apparatus Nucleolus Cilia Nucleus Vacuole Endoplasmic Reticulum Cytoplasm Centriole 18 A.________________________ B. ________________________ C. ________________________ D. ________________________ E. ________________________ F. ________________________ G________________________ H. ________________________ I. ________________________ J. ________________________ K. ________________________ Color the all the cytoplasm one color. Color all the organelles of each type a different color. 19 A. B. C. D. E. F. G. H. I. J. K. L. Color the all the cytoplasm one color. Color all the organelles of each type a different color. 20 Part F Moving in and moving out A molecule is the smallest part of a substance that is still that substance. Molecules are made up of tiny parts called atoms. Molecules are always moving. Most molecules move from place where they are crowded to places where they are less crowded. The movement of molecules from crowded areas to less crowded areas is called diffusion. For example if you open a bottle of perfume in a room, the molecules of perfume will diffuse throughout the room. In a beaker, dye molecules will spread through the water until the molecules of dye are evenly mixed. In order for a cell to carry on its life processes, oxygen and other substances must pass through the cell membrane. Wastes must be removed from the cell. The cell membrane has tiny holes in it. Substances can go in and out of the cell by moving through the holes. Some substances move into and out of a cell by diffusion. Molecules move in and out to keep the same amount of a substance on both sides of the cell membrane. However, the cell membrane only lets some substances in and out. If a molecule of a substance is too big, it cannot pass through the cell membrane. The movement of water through a membrane is called osmosis. Osmosis is a special kind of diffusion. Many substances dissolve in water before they move into a cell. Water moves through a cell membrane by osmosis. Osmosis causes cells to change their size. They can either get bigger, smaller or stay the same size depending on what type of solution they are sitting in. 21 If a cell is sitting in a solution that has more water outside the cell than inside the cell, the solution is called hypotonic. When a cell sits in a hypotonic solution, water will enter the cell until the concentration of water is the same inside the cell as outside. The cell will swell. Sometimes getting equal concentrations is not possible so water will keep entering until the cell bursts. This is shown in the picture to the right. If a cell is sitting in a solution that has a lower concentration of water outside the cell than inside the cell, the solution is called hypertonic. When a cell sits in a hypertonic solution, water will leave the cell until the concentration of water inside the cell will equal the concentration outside. This causes the cell to shrivel up. You can see this in the picture to the left. If a cell is sitting in a solution that has the same concentration of water inside as out, the solution is said to be isotonic. Water will go in the cell and leave the cell at the same rate. The cell will not grow or shrink. It will stay the same size. You can see a picture of a cell in an isotonic solution to the right. 22 Name:_____________________ Date:______________________ Part F Moving In and Moving Out Review 1. What is the movement of substances from where they are crowded to where they are not crowded called? 2. What is the movement of water through a membrane called? 3. Which cell organelle controls which substances can come into the cell and which can leave? 4. In which direction will carbon dioxide move if the amount of carbon dioxide in the cell is greater than the amount of carbon dioxide outside the cell? 5. Why would the smell of freshly baked bread eventually fill an entire house? 6. What happens to a cell that is in a hypertonic solution? 7. Which type of solution causes a cell to swell and possibly burst when the cell is put in the solution? 8. What type of solution causes water to enter and leave cells at the same rate? This type of solution does not cause the cell to change size. 23 Part G The Cell Cycle Cell can reproduce. They produce new cells. Living things grow because their cells can reproduce and make new cells. The process by which cells reproduce is called cell division. The cells of all living things are produced by cell division. Cell division only takes a very short time during a cells life. The rest of the time it is growing and making substance. The time when a cell is dividing and when its growing is called the cell cycle. The cell cycle is divided up into 3 main parts. The first part is called interphase. During interphase the cell is growing and making copies of its chromosomes. The DNA is spread out and you can not see chromosomes. This part is the longest part of the cell cycle. You can see a diagram of prophase to the right. The second part of the cell cycle is much shorter than interphase. It is called mitosis. During mitosis the nucleus divides. There are 4 stages in mitosis. The first stage is called prophase. During prophase, the chromosomes condense into thread like structures. The membrane around the nucleus disappears and the centrioles go to opposite sides of the nucleus. You can see a diagram of prophase to the left. The next stage is called metaphase. The chromosomes pair up and line up in the middle of the cell. Little fibers (that you can’t really see) show up between each chromosome and the centrioles. You can see a drawing of a cell in metaphase to the right. 24 Anaphase is next stage of mitosis. During anaphase the chromosomes begin moving toward the centrioles along the fibers. You can see a drawing of anaphase to the left. The last stage of mitosis is called telophase. During telophase the chromosomes reach opposite sides of the cell. For a short time, the cell looks like it has two nuclei. The rest of the cell has not divided yet, just the nucleus. You can see telophase in the drawing to the right. The last part of the cell cycle is the shortest part. It is called cytokinesis. During cytokinesis, the rest of the cell splits and becomes two daughter cells. Daughter cells are small cells just after cytokinesis. They are small because they have not gone through interphase when they do their growing. All cells go though the parts of the cell cycle just described. Plants however, have to form a new cell wall and a new cell membrane between the new nuclei. Animal cells only have to form a new cell membrane between the nuclei. 25 Name:___________________________ Date:____________________________ Part G The Cell Cycle Review Match the parts of the cell cycle with the things that happen during it. Please clearly write your answer in the left margin. If you would like to change your answer, cross it out and write your final answer next to it. a. Chromosomes arrive at opposite sides of cell, ____1. Anaphase cell looks like it has 2 nuclei b. chromosomes begin to move to opposite ____2. Cytokinesis sides of the cell along the fibers c. Chromosomes pair up and line up in the ____3. Interphase middle of the cell d. cytoplasm and other organelles split, new cell ____4. Metaphase membrane forms, new cell wall forms in plants as well e. DNA condenses into chromosomes you can ____5. Prophase see, nuclear membrane disappears f. growth of cell, DNA doubles, cell is making ____6. Telophase substances 7. Name the three main parts of the cell cycle. a. b. c. 8. Why do you think the chromosomes need to be copied during interphase if the cell is going to divide? 9. What is the name give to the small immature cells that are produced by cytokinesis? 10. How is cytokinesis different in plants than in animals?