Download GENETIC ENGINEERING QUESTIONS

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Document related concepts

Nucleic acid analogue wikipedia, lookup

Deoxyribozyme wikipedia, lookup

Point mutation wikipedia, lookup

Artificial gene synthesis wikipedia, lookup

Replisome wikipedia, lookup

Cre-Lox recombination wikipedia, lookup

Gel electrophoresis of nucleic acids wikipedia, lookup

Microevolution wikipedia, lookup

Designer baby wikipedia, lookup

Gene wikipedia, lookup

Nutriepigenomics wikipedia, lookup

Site-specific recombinase technology wikipedia, lookup

History of genetic engineering wikipedia, lookup

Genetic engineering wikipedia, lookup

Primary transcript wikipedia, lookup

Therapeutic gene modulation wikipedia, lookup

Helitron (biology) wikipedia, lookup

Non-coding DNA wikipedia, lookup

Vectors in gene therapy wikipedia, lookup

Epigenomics wikipedia, lookup

Cancer epigenetics wikipedia, lookup

Nucleosome wikipedia, lookup

DNA vaccination wikipedia, lookup

Genomics wikipedia, lookup

Extrachromosomal DNA wikipedia, lookup

Cell-free fetal DNA wikipedia, lookup

DNA supercoil wikipedia, lookup

No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia, lookup

Mutation wikipedia, lookup

Metagenomics wikipedia, lookup

Genomic library wikipedia, lookup

Mutagen wikipedia, lookup

Comparative genomic hybridization wikipedia, lookup

United Kingdom National DNA Database wikipedia, lookup

DNA polymerase wikipedia, lookup

Microsatellite wikipedia, lookup

DNA profiling wikipedia, lookup

DNA repair wikipedia, lookup

Zinc finger nuclease wikipedia, lookup

Genealogical DNA test wikipedia, lookup

Bisulfite sequencing wikipedia, lookup

Molecular cloning wikipedia, lookup

Nucleic acid double helix wikipedia, lookup

DNA damage theory of aging wikipedia, lookup

SNP genotyping wikipedia, lookup

Plasmid wikipedia, lookup

Transcript
GENETIC ENGINEERING QUESTIONS
BIO H
DR WEINER
Multiple Choice (6 pts)
1. A scientist wants to mass produce a recombinant form of a protein for human use.
He should first
a. Purify the protein
b. Clone the gene for the protein
c. Perform PCR on the protein
d. Grow bacteria to make the protein
2. People show restriction fragment length polymorphism because
a. They have single nucleotide differences in their DNA
b. The have different numbers of tandem repeats in their genes
c. Both a and b
d. Neither are correct
3. In gel electrophoresis smaller fragments of DNA
a. Move slower down the gel
b. Move faster down the gel
c. Move towards the negative charge of the gel
d. None are correct
4. RFLP is a technique used to
a. Identify a suspect that committed a crime
b. Identify a child’s father
c. Compare DNA among different species
d. All are correct
5. In a cloning experiment, the plasmid used to insert the gene of interest into has an
antibiotic resistant gene in it. When bacteria mixed with plasmid are grown in the
present of antibiotic
a. Only bacteria without the plasmid will grow
b. Only bacteria with the plasmid will grow
c. All bacteria will grow. The antibiotic does not affect the bacteria.
d. No bacteria will grow. Antibiotics kill bacteria.
6. Restriction enzymes cut DNA
a. Into individual nucleotides
b. St random locations
c. St short sequences specific to the restriction enzyme
d. Into equal pieces
The graph below shows the number of ACCURATE copies of DNA produced by PCR
7. What can you conclude about cycles 4 to 12?
a. PCR produced accurate copies at an exponential rate
b. The amount of DNA produced by PCR doubled with each cycle
c. The DNA copies produced by PCR were not accurate copies of the DNA
d. The rate at which PCR produced accurate copies fell in later cycles
8. Based on the graph, which of the following might have happened between cycles
12 and 13?
a. PCR stopped producing accurate copies of DNA
b. The rate of PCR increased
c. All of the template DNA was used up
d. A mutation occurred
Short Answer (10 pts)
1. Explain the process by which a foreign gene is placed into a bacterial cell and
expressed. Discuss some uses of the process.
2. Discuss how the polymerase chain reaction works.
3. Explain the process of gel electrophoresis.
4. Discuss the procedure used for DNA fingerprinting. What is the basis for DNA
fingerprints. How has DNA fingerprinting been used?
5. What are the tools used in genetic engineering? What is the function of each one?