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Multiple Choice (6 pts)
1. A scientist wants to mass produce a recombinant form of a protein for human use.
He should first
a. Purify the protein
b. Clone the gene for the protein
c. Perform PCR on the protein
d. Grow bacteria to make the protein
2. People show restriction fragment length polymorphism because
a. They have single nucleotide differences in their DNA
b. The have different numbers of tandem repeats in their genes
c. Both a and b
d. Neither are correct
3. In gel electrophoresis smaller fragments of DNA
a. Move slower down the gel
b. Move faster down the gel
c. Move towards the negative charge of the gel
d. None are correct
4. RFLP is a technique used to
a. Identify a suspect that committed a crime
b. Identify a child’s father
c. Compare DNA among different species
d. All are correct
5. In a cloning experiment, the plasmid used to insert the gene of interest into has an
antibiotic resistant gene in it. When bacteria mixed with plasmid are grown in the
present of antibiotic
a. Only bacteria without the plasmid will grow
b. Only bacteria with the plasmid will grow
c. All bacteria will grow. The antibiotic does not affect the bacteria.
d. No bacteria will grow. Antibiotics kill bacteria.
6. Restriction enzymes cut DNA
a. Into individual nucleotides
b. St random locations
c. St short sequences specific to the restriction enzyme
d. Into equal pieces
The graph below shows the number of ACCURATE copies of DNA produced by PCR
7. What can you conclude about cycles 4 to 12?
a. PCR produced accurate copies at an exponential rate
b. The amount of DNA produced by PCR doubled with each cycle
c. The DNA copies produced by PCR were not accurate copies of the DNA
d. The rate at which PCR produced accurate copies fell in later cycles
8. Based on the graph, which of the following might have happened between cycles
12 and 13?
a. PCR stopped producing accurate copies of DNA
b. The rate of PCR increased
c. All of the template DNA was used up
d. A mutation occurred
Short Answer (10 pts)
1. Explain the process by which a foreign gene is placed into a bacterial cell and
expressed. Discuss some uses of the process.
2. Discuss how the polymerase chain reaction works.
3. Explain the process of gel electrophoresis.
4. Discuss the procedure used for DNA fingerprinting. What is the basis for DNA
fingerprints. How has DNA fingerprinting been used?
5. What are the tools used in genetic engineering? What is the function of each one?