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Transcript
Chapter 15-16 Packet 1. Outline 15a 2. Outline 15b 3. Outline 16a 4. Outline 16b 5. Ch.15 Review 6. Ch.16 Review 7. 4 X 6 card Ch.15-16 Review Know difference between in vivo and ex vivo gene therapy Know what restriction enzymes, “sticky ends” are, their function, and how they are useful in recombinant technology, where restriction enzymes come from Know the functions of promoter, operator, enhancer, regulator sites on DNA Know the different levels of control/their order in gene activity/expression in eukaryotes Know everything about the lac operon; iducible /repressor operon and CAP transcription activator Know everything about the trp operon; repressible operon, repressor Know how gene control/regulation differs in prokaryotes and eukaryotes Know how different types of mutations affect translation differently, point mutations, frameshift mutations Know exactly how sickle cell anemia is caused Know how mutations in certain genes can lead to cancer, factors that increase likelihood of developing cancer, examples of carcinogens Know what “jumping genes” are Know the steps used to clone genes in bacteria producing recombinant DNA Know how DNA ligase is used in recombinant technology Know what PCR is and is used for Know what gel electrophoresis is and what it is used for Know what barr bodies, heterochromatin and euchromatin are Know how reverse transcriptase is useful in cloning genes, where it comes from, what cDNA is Know why bacteria cannot translate eukaryotic genes, and how this is overcome Know various examples of transgenic plant/products Know what is meant by a vector in biotechnology Know examples of uses of biotechnology Know terms: bioinformatics, genomics, proteomics Know what gene therapy is, and how it has been used Free Response Know the steps of how to create recombinant DNA/genetic engineering/cloning genes to produce desired gene products/genes Example 98-2 on sample essays handout would be a good example to know
