Download Gene Expression - Phillips Scientific Methods

NAME: ____________________________
PERIOD: _____
DATE: _______________
General Questions – Gene Expression
1. What is the purpose of replication?
2. What is the purpose of transcription?
3. What is the purpose of translation?
4. Where does replication occur in eukaryotic cells?
5. Where does transcription occur in eukaryotic cells?
6. Where does post-transcriptional processing occur in eukaryotic cells?
7. Where does translation occur in eukaryotic cells?
8. Circle all that apply
a) DNA Polymerase is used in
Replication
Transcription
Translation
b) RNA Polymerase is used in
Replication
Transcription
Translation
c) Pyrophosphate powers
Replication
Transcription
Translation
d) ATP & GTP powers
Replication
Transcription
Translation
9. Describe the role of each of the enzymes discussed in Replication
10. Describe 3 ways that the RNA that leaves the nucleus differs from a primary transcript?
11. Define each of the following, and explain what type of point mutation could cause each of these
mutations.
a) Silent mutation
b) Missense mutation
c) Nonsense mutation
d) Frameshift mutation
NAME: ____________________________
PERIOD: _____
DATE: _____________
You are given the following DNA in order to be made into a protein.
5’ end
ATG TCT ATA AGC AAA CGA CAA GGC GGT TAA 3’ end
3’ end
TAC AGA TAT TCG TTT GCT GTT CCG CCA ATT
5’ end
1. Write out the sequence of BOTH products of replication. What do you notice about these
products?
2. Which strand is the coding strand and which is the non-coding strand in the DNA molecule
above? (Label the strands on the diagram above)
3. Write out the mRNA that would be transcribed from the coding strand above, be sure to
specify both the 5’ and 3’ ends
4. Write out the sequence of tRNA anti-codons that is complementary to the mRNA sequence
above:
5. Translate the mRNA into an amino acid chain using the 3-base codons on the mRNA molecule
you wrote in the space above. You may need to use codon chart on pp. 330
NAME: ____________________________
PERIOD: _____
DATE: _______________
Comparison Chart
Replication
Where?
What is used as the
template?
What is the new
strand composed of?
What is used to
synthesize the new
strand?
Which Nucleotides
are involved?
Where does the
finished product go?
What happens in
Post-Processing?
Transcription
Translation
NAME: ____________________________
PERIOD: _____
DATE: _______________
Gene Expression
Arrange the following by their occurrence in protein synthesis or gene expression.
_____ A release factor binds to the “A” site of the ribosome.
_____ The ribosome falls off the mRNA.
_____ RNA Polymerase binds to the DNA strand to complete the transcription initiation complex.
_____ The ribosome reaches UAA, UAG or UGA.
_____ snRNPs attach to the beginning and ending sequences of introns.
_____ Using GTP for power, the tRNA in the “A” site moves to the “P” site.
_____ A guanine cap and a 50-250 nucleotide Poly–A tail are added to the mRNA.
_____ The tRNA of the “P” site moves to the “E” site and then leaves the ribosome.
_____ The small subunit of the ribosome binds to the leader segment of mRNA and a
methionine tRNA.
_____ GTP is used to attach the large subunit of the ribosome to the mRNA initiation complex.
_____ The next tRNA matches its anti-codon to the codon of the “A” site.
_____ Spliceosome adheres to snRNPs and excises introns while sealing exons into a
continuous strand of mRNA.
_____ Two GTPs are used to transfer the amino acid onto the polypeptide in the “A” site.
_____ RNA Polymerase pulls the double helix strands apart 10-20 base pairs at a time to reveal
the nucleotide sequence of the template strand.
_____ RNA nucleotides are added to the 3’ end of the growing strand of mRNA at the rate of ~60
per second.
_____ mRNA leaves the nucleus through a nuclear pore.
_____ The ribosome continues to move from the 5’ end to the 3’ end, adding amino acids to the
growing polypeptide chain.
_____ The termination sequence is transcribed and the RNA Polymerase falls off.