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Gramene v.19 Welcome to the Gene and Allele Database Tutorial This tutorial will describe how to navigate the section of Gramene that provides descriptions of alleles associated with morphological, developmental, and agronomically important phenotypes and variants of physiological characters, biochemical functions and isozymes. Many genes in Gramene also have map positions and sequencing data. e a r c h Gene Gramene ID Alphabetical Browse M a p Gene Symbol Term Search Chromosome Synonym Phenotypic Description Literature General Information Gene Name References Phenotypic Image Allele Summary Allele and Germplasm Olryzabase Phenotypic Description Study Name Study Info Study Type Study location Study year Allelic Interaction Germplasm Summary Wildtype Location Allele Description Germplasm Accession Mutagen Mutants studied Study references Allele symbol Study Season NCBI Map and Sequence GenBank Accession Protein Database Mutagenesis Method Gene Product Map Set Name Associated Features Map Position Linkage Group Start position Trait Anatomy location Evidence code Developmental stage Ontology Stop position Tutorial Help The hand icon indicates a link that allows you to go to the same page in your web browser. If you are viewing this tutorial with Adobe Acrobat Reader, click the "bookmarks" on the left hand side of the Reader for easier navigation. Action Options are noted in this type of font. Notes or comments use this style font. Gramene Home Page Click here to open gene search Gene & Allele Database Home Page Click here to open search page Step one: Search Genes 4 methods 1. Type either a gene symbol, a gene name, a Gramene Association or a keyword. Entering “%” will result in displaying the entire gene list. 1a. Click “Search” to view the gene detail page OR 2a. Click “alphabetical order” to view the entire gene list. 2b. Or select the letter of the desired subset (such as “D” for dwarf.) 3. Click to browse genes by searching for a trait in the Ontology Database 4. Click to browse genes by searching literature citations in the Literature Database Step Two: Results for Gene Search by Term / Key word There are 82 entries for “dwarf”, with 25 entries per page 2. Click on a gene name to view the detail page for that particular gene. 1. Click to view next page of results as needed. Step Two: Results of Gene Search for Alphabetical Search “D” 1. Click on a column title to sort results by that column. 2. Click on a gene name to view the detail page for that particular gene. 3. You may wish to select a letter to view another subset list (such as “S” for semi-dwarf.) Step three: Gene Detail Page General Info for Gene Select desired detailed info from menu and click. Click a thumbnail to enlarge image. Step three: Gene Detail Page Allele and Germplasm Click to view summary of allele. Click to view summary of the germplasm Step three: Gene Detail Page Allele and Germplasm View of Allele Summary Page Select for more detailed information on the referenced study. Select to return to gene detail page. Step three: Gene Detail Page Allele and Germplasm View of Allele Study Page Select to view related references. Select for detailed information on other genes in the study. Step three: Gene Detail Page Allele and Germplasm View of Germplasm Summary Page Select to return to gene detail page. Step three: Gene Detail Page Map and Sequence Info Click to view the nucleotide sequence link for each from the National Center for Biotechnology Information. Select to view more information from Gramene’s Protein database (See Protein tutorial). Select to view the gene displayed in CMap (See CMap tutorial). Step three: Gene Detail Page Associated Features Click to sort by Trait ontology Click to sort by Developmental Stage Click to go to the Trait Ontology Database within Gramene (See Ontology tutorial). Click to go to the Plant Ontology Database within Gramene. (See Ontology tutorial). Step three: Gene Detail Page References Click to view Oryzabase’s information on the selected gene. Click to go to the related reference in Gramene Literature database (See Literature tutorial). How to Submit Newly Identified Genes Contact Gramene Use the feedback button, located at the top of every page, to provide feedback or to ask questions about Gramene. or Email Gramene at [email protected]