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Warm ups: 1. What are the four bases that make up DNA? 2. What is a mutation? 3. List the different types of mutations… DNA Transcription & Protein Translation Today’s Objectives 1. To explain how DNA and RNA code for proteins and determine traits. 2. To investigate and understand common mechanisms of protein synthesis. Making Proteins DNA directs the formation of proteins by the ribosomes of the cell. RNA – Ribonucleic acid. The molecule that carries information from nucleus (DNA) to ribosomes RNA is different for DNA: RNA only has one side of the ladder shape RNA’s sugar is ribose (DNA's deoxyribose) RNA has the nucleotide base Uracil (U) in place of Thymine (RNA also contains the other three bases: A, C and G). When forming base pairs, C links with G, but A links with U (no T) Transcription 1. DNA must be copied to messenger RNA (mRNA) What would be the mRNA strand that is copied from the DNA strand below? Remember, RNA has U instead of T DNA Sequence G A C U U A mRNA Sequence 2. mRNA goes from nucleus to the ribosomes in cytoplasm Transcription 3. mRNA complements known as codons – Only 3 nucleotide “letters” long Transcription – Step I A C G T A T C G C G T A T G C A T A G C G C A T Template DNA Strands Transcription – Step II A C G T A T C G C G T A U G C A U A G C G C A U Template DNA is Matched Up with Complementary mRNA Sequences Transcription – Step III A C G U A U C G C G U A U G C A U A G C G C A U mRNA leaves nucleus and goes to ribosomes A new complementary RNA strand is made (rRNA) Protein Translation 1. mRNA leaves the cell nucleus goes to ribosome 2. mRNA code is translated into a transfer RNA (tRNA) code which, in turn, is transferred into a protein sequence. Protein Translation 3. Each set of 3 mRNA bases (codons) will pair with a complimentary tRNA base triplet (called an anticodon). 4. Each tRNA is specific to an amino acid, as tRNA's are added to the sequence, amino acids are linked together by peptide bonds, eventually forming a protein that is later released by the tRNA. 5. Codon code is specific, but redundant! 20 amino acids 64 triplet (codon) combinations Protein translation - example Using the mRNA strand we obtained above, we generated the following amino acids mRNA codon UUA After the processes of transcription and translation are complete, we are left with a protein that consists of the chain: – GAC Aspartic Acid - Leucine Although our 'protein' is only 2 amino acids in length, proteins normally consist of hundreds or thousands of amino acids. tRNA structure 3-base code (triplet) is an “anticodon” Protein molecule Attached amino acid that is carried from cytoplasm to ribosomes Protein Synthesis 1. Start: Ribosome binds to mRNA at start codon 2. Elongation: – – – tRNA complexes bind to mRNA codon by forming complementary base pairs with the tRNA anticodon The ribosome moves from codon to codon along the mRNA. Amino acids are added one by one 3. Release: release factor binds to the stop codon Exit slip 1. 2. 3. List the three types of RNA? What does RNA stand for? What organelle in the cell is the place for protein production?