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Review 3 C483 Spring 2013 Net outcomes of • • • • • Oxidative phosphorylation (shuttles) Fatty acid catabolism Nitrogen catabolism Light reactions of photosynthesis Calvin cycle Structures to know • All amino acids • (deoxy)ribonucleosides and (deoxy)ribonucleotides • Carbamoyl phosphate and urea • Pyruvate, oxaloacetate, a-ketoglutarate • PRPP Metabolic Pathways/Reactions • Know these reactions/pathways (not arrow mechanisms) – Beta oxidation – PLP-mediated transamination – Formation of carbamoyl phosphate – Catabolism of amino acids covered in lecture – Basic hydrolysis and RNase-catalyzed hydrolysis of RNA Protonmotive Force • NADH + H+ + ½ O2 NAD+ + H2O + 10 H+ pumped • succinate + ½ O2 fumarate + H2O + 6 H+ pumped Photosynthesis: Net Reaction 2 H2O + 4 NADP+ + 8 photons O2 + 4 NADPH Proton Gradient • For every ONE O2 – 4 e- passed – 8 photons absorb – 12 H+ pumped • In plants, 12 H+ required to turn ATP synthase – 3 ATP made • Quantum yield is 3 ATP/8 photons or ATP/2.7 photon