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Transcript
Primer extension • * This labelling technique uses random oligonucleotides (usually hexadeoxyribonucleotide moleculessequences of six deoxynucleotides) to primer synthesis of a DNA strand by DNA polymerase. • * The DNA to be labelled is denaturated by heating, and the oligonucleotide primer annealed to the single stranded DNAs. •The klenow fragment of DNA polymerase can synthesize a copy of the template, primed from the 3-hydroxyl group of the oligonucleotide. * If a labelled dNTP is incorported, DNA of very high specific activity is produced. (Fig 12) Nucleic acid hybridization •The complementary feature of DNA base-pairing can be used to provide information a bout the sequence complexity of the DNA. •If a DNA duplex is denaturated by heating until the strands separate, the complementary strands will be renaturated on cooling . (Fig 13)
