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Chapter 2 Structure and function of cells Plasma membrane Movement (diffusion/Osmosis) Surface area to volume ratio Organelles Tissues/Organs Plasma membrane Environments of cells • All cells exist in a watery environment – extracellular fluid • Mullticellular organisms have an outer layer that acts as a barrier and creates an internal environment Aspects of the internal environment that need to be regulated: •Salt concentrations •Temperature •Levels of nutrients •pH •Removal of wastes •Ion concentrations CELL MEMBRANE The role of the membrane is very important in maintaining cell stability and enabling the cell to operate at optimum levels. The cell membrane (plasma membrane), - selectively controls the molecules entering & leaving the cell. - allows the cytoplasm to have a different composition from the the surrounding environment Membrane Structure. • The membrane is partially permeable. Some substances pass freely across the membrane while others are excluded. Phosopholipid bi-layer • The cell membrane is formed from a double layer of lipids with proteins embedded in it forming ion channels. • The lipid layer is capable of much movement, i.e. it is fluid. • Cholesterol molecules reduce the membrane fluidity • It is impermeable to water soluble substances. • Lipid soluble substances e.g. alcohol pass through. Hydrophobic, ‘water hating’ end Hydrophillic, When in contact with an aqueous solution, the phospholipid molecules line up with tails pointing in (away from sol) ‘water loving’ end EXAMPLE! Detergents cause fats to form micelles. Protein Channels • These are large proteins with openings on both sides of the membrane. • Allow flow of ions •Carbohydrates on the outer surface are important in cell adhesion and cell recognition. These are attached to recognition proteins. (1) Fluid (2) Mosaic Model (Draw) Carbohydrate Embedded protein Phospholipid Bilayer Embedded Cholesterol Protein channel Movement into and out of the cell Movement in and out of Cell • Diffusion (simple) • Osmosis • Facilitated diffusion • Active Transport • Endocytosis and Exocytosis Diffusion (simple) Where? What? Through the small uncharged particles phospholipid (water, urea, Oxygen, carbon bilayer dioxide) & lipid soluble substances such as alcohol, ether, chloroform. Energy? NO Comments: Moves down Con~ gradient (high to low) The diffusion rate increases with a high concentration gradient, heat, small particles and with gaseous substances. Movement is from a strong solution to a weak solution. (think sugar cube /water) (lungs, CO2) Osmosis Where? Through the phospholipid bilayer What? Water only Energy? NO Comments: Osmosis is a special type of diffusion. The process is the same but only water moves to even up concentrations. Movement of water is from a weak solution into a strong solution. Example: water from the gut (diarrhoea) Cell wall in plants Diffusion across the (partially permeable) membrane. 1 2 Active Transport. Where? Through the protein molecules spanning the membrane. What? Nutirent molecules, ions such as chloride ions (Cl-), sodium ions (Na+), potasssium ions (K+) and water Energy? Yes Comments: Active transport is movement against the concentration gradient to ‘build up’ rather than to‘even up’. Bulk Transport (endocyctosis and exocytosis). Where? Small sections of the plasma membrane. What? Large molecules such as enzymes, hormones and antibodies and foreign material. Energy? YES Comments: Phagocytosis (solids - cell eating) and pinocytosis (cell drinking – entry of liquid) are forms of endocytosis. Summary of common substances and their passage through the cell membrane Surface area to volume ratio Surface area to volume ratio Organelles CELL THEORY All living things are made up of one or more cells. All cells are formed from pre-existing cells and these cells contain the hereditary information. The cell is the smallest organisational unit Organisation Atom /molecules cells tissues organs organ systems individual population and community ecosystem Properties of cells • Most cells have some common features: Plasma membrane Cytoplasm Nucleus Flagella or Cilia Types of cells • There are two types of cells • Organisms are grouped according to what type of cell they have • Prokaryotes – have cells that do not have a membrane surrounding the nucleus and lack most organelles (unicellular or simple multicellular organisms - Bacteria, cyanobacteria) (pic pg 23) • Eukaryotes- have cells that are usually much larger and more complex than prokaryote cells. The nucleus is surrounded by a membrane and the cell contains organelles (protists, fungi, plants & animals) Cell Organelles Overview Mitochondrion • Cells require a continuous supply of energy. • Organelles where: – food molecules are broken down. – energy is released. • The energy is then stored in other molecules that can power cell reactions easily. • Mitochondria (plural) – use when you refer to more than one mitochondrion. Nucleus • Usually the largest organelle in the cytoplasm of a cell. • Directs all the activities of the cell. • Contains genetic blueprints for the operations of the cell. Centrioles • • • • Self-replicating organelles Made up of nine bundles of microtubules Found only in animal cells. Help in organizing cell division. Cell membrane • Forms the outer boundary of the cell. • Allows only certain materials to move into and out of the cell. • Food, oxygen and water move into the cell through the membrane. • Waste products also leave through the membrane. • Cells that perform photosynthesis (plants and some protists) take in carbon dioxide through the cell membrane instead of oxygen. Cell wall • The cell wall is a rigid structure outside the cell membrane that supports and protects the cell (for plants, fungi, and some protists and bacteria but NOT animals). • Animal cells DO NOT have a cell wall. • The cell wall is made of tough cellulose fibres and other materials made by the cell. • Note: fungal cell walls contain chitin instead of cellulose. Cytoplasm • Gel-like material inside the cell membrane and outside the nucleus. • Cytoplasm is the jelly-like material inside the cell (but unlike gelatin it does flow; cytoplasm constantly moves or streams. • Contains a large amount of water (cytosol) and many chemicals and structures that carry out the life processes in the cell. • The structures that the cytoplasm contains are called organelles. Endoplasmic reticulum • A folded membrane that moves materials around in the cell. • Extends from the nucleus to the cell membrane and takes up quite a bit of space in some cells. • Two different types: Smooth ER and Rough ER. Rough ER has ribosomes attached to its outer membrane, while Smooth ER does not. Golgi bodies Golgi bodies package and move proteins • • In a business, products are made, packaged, and moved to loading docks to be carried away. • In cells, structures called Golgi Bodies are stacks of membrane-covered sacs that package and move proteins to the outside of the cell. • When something is secreted, it is given off by the cell. • Note: Golgi Bodies are sometimes referred to as Golgi Apparatus. Vesicle • Vesicles transport proteins. Chloroplasts • Chloroplasts take in sunlight, water and carbon dioxide to make oxygen and sugar. • This process is called photosynthesis. • A plant's chloroplasts convert light energy into chemical energy. • Contain a green pigment called chlorophyll. This is what makes plants green. Vacuole • Acts as a temporary storage space for the cell. • Vacuoles store water, food, pigments, waste or other materials. • Vacuoles are large in plant cells and small in animal cells. • Vacuoles can also be found in fungi and protists. Lysosomes • An active cell constantly produces waste products. • Contain chemicals (enzymes) that: – digest wastes and worn-out cell parts – break down food. Ribosome • One chemical that takes part in nearly every cell activity is protein. • Proteins are needed for chemical reactions that take place in the cytoplasm. • Cells make their own proteins on small structures in the cytoplasm called ribosomes. Cilia and Flagella • For single-celled eukaryotes, cilia and flagella are essential for the locomotion of individual organisms. • In multicellular organisms, cilia function to move fluid or materials past an immobile cell as well as moving a cell or group of cells. – Cilia lining nasal passage – Sperm Microfilaments • Solid rods made of globular proteins called actin and are common to all eukaryotic cells. • Long chains of the molecules are intertwined in a helix to form individual microfilaments. • Filaments are primarily structural in function and are an important component of the cytoskeleton, along with microtubules. • In association with myosin, microfilaments help to generate the forces used in cellular contraction and basic cell movements. • They enable a dividing cell to pinch off into two cells and are involved in amoeboid movements of certain types of cells. • They also enable the contractions of muscle cells. Microtubules• Straight, hollow cylinders are found throughout the cytoplasm of all eukaryotic cells (prokaryotes don't have them) and perform a number of functions. • Gives structure and shape to a cell, • Serve as conveyor belts moving other organelles through the cytoplasm • Are the major components of cilia and flagella, and participate in the formation of spindle fibers during cell division (mitosis). • These filaments are composed of linear polymers of tubulin, which are globular proteins, and can increase or decease in length by adding or removing tubulin proteins. Plant cell Animal cell Copy down the characteristics of cells in the 5 kingdoms pg 26 HW - Q 1- 8