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Chapter 7 A View of the Cell Cell Theory • CD-ROM • Before microscopes, people thought diseases were caused by curses and supernatural spirits – They had no idea microorganisms, such as bacteria and viruses existed • When the microscope was invented, scientists discovered cells exist • Cells are the basic units of living organisms Cell Theory • Anton van Leeuwenhoek (Dutch) used the first simple light microscope in the 1600s – It had one lens (page 1064) • Gradually, lenses got better • Compound light microscopes use a series of lenses to magnify objects in steps – Can magnify up to 1500 times – The microscopes we use in the lab Cell Theory • Robert Hooke (1600s, English) used a compound light microscope to study cork, the dead cells of oak bark • He saw small, geometric shapes that reminded him of the small rooms monks lived in called cells • Cells are the basic units of all living things Cell Theory • Matthias Schleiden (1830s, German) discovered that all plants are made of cells • Theodor Schwann (German) discovered that all animals are made of cells • Rudolf Virchow discovered that all cells come from existing cells • All these scientists (plus others) contributed ideas that are now summed up in what we call the cell theory Cell Theory 1. All organisms are composed of one or more cells. 2. The cell is the basic unit of structure and organization of organisms. 3. All cells come from preexisting cells. Electron Microscopes • Electron Microscopes let us see a much greater magnification than light microscopes – They use beams of electrons to produce images • Scanning Electron Microscope – Used for scanning 3-D surfaces Electron Microscopes • Transmission Electron Microscope – Used for scanning interior structures of cell Types of Cells • Organelles are small, specialized structures within cells – Many, but not all are surrounded by membranes • Prokaryotes are organisms that do not have membrane-bound organelles (biology.arizona.edu) – Kingdom Monera ( now split into Eubacteria & Archaebacteria) • Eukaryotes are organisms that do have membrane-bound organelles – Kingdoms Animalae, Plantae, Fungi, Protista Types of Cells • Robert Brown (Scottish) observed that eukaryotic cells have a prominent structure, the nucleus – the nucleus manages cellular functions • Rudolf Virchow concluded that this prominent structure was responsible for cell division Section 1 Review 1. How has the history of microscopes influence the study of cells? 2. What are the three parts of the cell theory? 3. What is the difference between a light microscope and an electron microscope? 4. What is the difference between a prokaryote and a eukaryote? The Plasma Membrane • Cells must maintain proper conditions within itself to function • The plasma membrane is a flexible boundary between the cell and its environment – It allows a steady supply of nutrients into and out of the cell at the appropriate levels • The plasma membrane is selectively permeable – This means that it allows some molecules to pass through while keeping others out. – Like a screen in a window The Plasma Membrane • Phospholipids • The “head” is polar, or slightly charged – It is hydrophilic (likes water) • The “tail” is nonpolar, or not charged – It is hydrophobic (repels water) • The phospholipids arrange themselves form a bi-layer with the fatty acid tails forming the interior and the heads facing the watery environments outside and inside the cell Plasma Membrane • The plasma membrane is called a phospholipid bilayer • Arranged in this manner, a barrier is created that is water-soluble at its outer surfaces and waterinsoluble in the middle • Keeps out both water soluble and water insoluble molecules Plasma Membrane • The current model of the plasma membrane is called the fluid mosaic model. • The phospholipids actually move around like a fluid. • The other components of the membrane (proteins, carbohydrates, cholesterol, etc.) move around as well Plasma Membrane • Cholesterol helps to stabilize the phospholipids by preventing their fatty acid tails from sticking together • Transport proteins move span the membrane and move needed substances or waste materials through the plasma membrane • Other proteins and carbohydrates that stick out help cells to identify surface signals and other cells – These proteins play an important part in protecting cells from infection Section 2 Review 1. 2. 3. 4. What is a phospholipid? Why is the cell membrane a bilayer? What does polar and nonpolar mean? What are the specialized parts of the cell membrane? 5. Why is the cell membrane referred to as fluid mosaic? Cell Wall • Fairly rigid structure located outside the plasma membrane of some cells that provides additional support and protection – Plants, fungus, most bacteria • The cell wall is very porous, made of a tough mesh of fibers – Like framing of a house Nucleus • Membrane-bound (called nuclear envelope) organelle that contains the directions to make proteins • Nuclear pores allow passage through the nuclear envelope • Chromatin - uncondensed strands of DNA – When cell is dividing, DNA condenses into chromosomes • Nucleolus - organelle within the nucleus that make ribosomes Organelles • Ribosomes are the sites where the cell produces proteins according to the directions of DNA – One organelle without a membrane • Cytoplasm is the clear, gelatinous fluid inside the cell – Acts as a medium for things to move around in the cell – “Organelles can’t fly” Organelles • The endoplasmic reticulum (ER) is arranged in a series of highly folded membranes suspended in the cytoplasm • In general, ER is involved in the movement of materials throughout the cell • Rough ER has ribosomes, which is where protein synthesis takes place • Smooth ER has no ribosomes and is involved in numberous biochemical activities, including the production and storage of lipids Organelles • The Golgi apparatus is a flattened stack of tubular membranes that modifies the proteins • The Golgi sorts proteins into packages and packs them into membranebound structures called vescicles for later transport (like UPS) Organelles • Vacuoles are membrane-bound compartments for temporary storage of materials – Store food, enzymes, water, waste – Plant cells have one very large vacuole Organelles • Lysosomes are organelles that contain digestive enzymes • Digest old organelles, food, viruses, bacteria, etc. • Can fuse with a vacuole to dump waste or give enzymes • Lysosomes digest a tadpole’s tail Organelles • Plastids are a group of organelles used for storage – Named for the color or pigment they contain • Chloroplasts are organelles that capture light energy and convert it to chemical energy (photosynthesis occurs here) – Plastid containing chlorophyll, which actually traps the light and gives plants green color Organelles • Mitochondria are membrane-bound organelles in plant and animal cells that transform stored chemical energy into a usable form for the cell (ATP) • Has an outer and inner highly folded membrane – ATP produced on inner folds Organelles • The cytoskeleton forms a framework for the cell – A network of tiny rods and filaments • Cytoskeleton is constantly changing its shape • Microtubules are thin, hollow cylinders made of protein • Microfilaments are smaller, solid protein fibers • Give support for cell • Provide “highway system” for organelles to move throughout cell Organelles • Centrioles are organelles made up of microtubules that play a part in cell division – In animals and most protists • Cilia are short, numberous projections that look like hairs – Made of microtubules and help organelles move and feed • Flagella are longer projections that move in a whip-like motion – Made of microtublules - help with movement – Cells usually only have 1 or 2 Animal Cell Plant Cell Section 3 Review 1. 2. 3. 4. 5. 6. 7. How are highly-folded membranes an advantage for the functions of cellular parts? Name an organelle that has highly-folded membranes. If a cell synthesizes large quantities of protein molecules, which organelles might be numerous in that cell? A cell’s digestive enzymes are enclosed in a membrane-bound organelle. How can these molecules function in the cell? Compare and contrast the functions of a cell wall to the functions of a plasma membrane. What are the functions of the cytoskeleton? Why are mitochondria and chloroplasts referred to as energy transporters?