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Chapter 7Cell Structure & Function I. Life is Cellular A-The Discovery of the Cell • • It was not until the _________ that scientists began to use microscopes to observe organisms. In 1665 ____________used an early compound microscope to see tiny chambers in cork.He called these chambers cells after the tiny rooms in monasteries….we know these not to be empty now. Mid-1500’s Robert Hooke • About the same time in Holland________________used a singlelens microscope to look @ pond water, Anton van Leeuwenhoek • In 1838 Matthew Schleiden concluded plants were made of cells • 1839 Theodore Schwann said all animals were made of cells • 1855-Virchow said cells could only come from existing ones. These 3 things compile_________________ – All living things composed of ___________ – Cells are the basic units of ___________________of living things – New cells are produced from ______________________. Existing cells cells Structure and function B-Exploring the Cell • Most microscopes use lens to magnify the image of a specimen with light or electrons • _________________,which scans cells w/a laser beam can make 3-d images of cells • Video technology make it possible to watch cell growth , division and development Confocal light microscopy • Light makes it difficult to visualize tiny structures because it scatters/______________________allow things like proteins to be visualized (things as much as 1000 x smaller can be visualized….TEMS allow you to see specimens cut into ultra thin slices Electron microscopes • With light microscopes stains need to often be used since most living cells are transparentsome stains are structure specific • Some dyes show fluorescence dyes give off light of a ceratin color when viewed under specific wavelenghths • May be able to track specific molecules • W/ a ______________specimens do not have to be cut to see 3-D images….both must be placed into a vacuum so air molecules do not scatter electrons/TEM-shows details • 1990’s____________________________have revolutionalized visualization of surfaces and atoms have been observed…can be used in ordinary air and can show DNA structure SEM Scanning probe microscopes pollen C .Prokaryotes and Eukaryotes • Cells typically range from _________micrometers,but some bacteria are .2 and some amoeba are 1000 micrometers • All cells have 2 things in common: » cell membrane-a barrier » @ some point they contain_______ 5-50 micrometers DNA 2 broad categories: – _____________________________genetic material is NOT contained in a nucleus/generally less complicated than other cells/carry out all cell activities…present day members are ________________. – Only organelles are ribosomes and they are NOT MEMBRANE BOUND Prokaryotes bacteria • • • _____________________________contain a nucleus w/ genetic material,generally larger,much diversity HAVE all organelles/most membrane bound Include all organisms EXCEPT bacteria Eukaryotes Division of Labor Section 7-2 •A cell is made up of many parts with different functions that work together. Similarly, the parts of a computer work together to carry out different functions. •Working with a partner, answer the following questions. •1. What are some of the different parts of a computer? What are the functions of these computer parts? •2. How do the functions of these computer parts correspond to the functions of certain cell parts? Go to Section: Venn Diagrams Section 7-2 Prokaryotes Cell membrane Ribosomes Cell wall Animal Cells Lysosomes Go to Section: Plant Cells Cell membrane Ribosomes Nucleus Endoplasmic reticulum Golgi apparatus Vacuoles Mitochondria Cytoskeleton Cell Wall Chloroplasts Eukaryotes Nucleus Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Mitochondria Cytoskeleton II. EUKARYOTIC CELL STRUCTURE • Organelles • 2 major parts of eukaryotic cells nucleus cytoplasm Specialized structure that performs important functions within an eukaryotic cell. /”little organs”/ Cytoplasm is material inside membrane and outside nucleus The Nucleus • Contains nearly all the cell’s DNA • Codes for instructions to make proteins and other molecules • Surrounded by nuclear envelope---has many pores to allow material in and out • Contains chromatin—has DNA bound to protein,usually spread throughout nucleus,but condenses during cell division to make CHROMOSOMES,containing genetic info • Usually contain Nucleolus—assembly of ribosomes begin here. Organelles That Store , Cleanup and Support • Vacuoles • Sac like structures that store water ,salts ,proteins, and carbs • Plants may have a single large water filled vacuole • Contractile vacuoles control water in paramecium • VESICLES-store and move between organelles and cell surface • Lysosomes • Small organelles filled w/enzymes • May digest or break down lipids,carbs,and proteins into small molecules that can be used by the rest of the cell • Lysosomes remove “junk”,or used up organelles…-very important that this aspect / function occurs • May be in some/ very few plants Figure 7-7 Cytoskeleton Section 7-2 Cell membrane Endoplasmic reticulum Microtubule Microfilament Ribosomes Go to Section: Michondrion Cytoskeleton • Network of protein filaments that help cell maintain shape • Also involved in movement • MICROFILAMENTS are threadlike structures made of a protein-actin….make a major network and a tough framework///allows amoebas and such to move • MICROTUBULES-hallow structures made of proteins called tubulins—important in holding a cell’s shape---form a mitotic spindle in cell division/which helps separate chromosomes • CENTRIOLES are microtubules near nucleus in animals and help organize cell division • Microtubules also help make projections like cilia or flagella • Arranged in “9+2” pattern of microtubules ORGANELLES THAT BUILD PROTEINS • • • • • Ribosomes : Proteins are assembled here Made out of small particles of RNA and protein Found throughout cytoplasm Coded instructions from nucleus tell how to make proteins • Cells active in protein synthesis have a lot of ribosomes ER • Endoplasmic Reticulum • ER-Site where lipid components of cell membrane are assembled ,along w/ proteins and other materials exported from cell(those proteins are made there) • Rough ER is involved in protein synthesis,because ribosomes are on it-finishes twisting and folding • Smooth ER involved in lipid metabolism and detoxifying poisons • Newly made proteins leave ribosomes and insert on rough ER ,where they may be modified • If cell makes a lot of protein ,there is much ER • Smooth ER may contain many specialized enzymes GOLGI BODY: • proteins from rough ER go here in this stack of membranes/get “address tags” to package and export to correct placebundled in vesicles • Golgi modifies ,sorts , packages proteins and other materials from ER for storage or release from cell ORGANELLES THAT CAPTURE AND RELEASE ENERGY • ==Mitochondria and Chloroplasts • Most all eukaryotic cells contain mitochondria that convert chemical energy stored in food into compounds convenient for cell to use • Mitochondria have an outer and inner membranes • In humans,nearly all mitochondria comes from ovum(egg cell) • Chloroplasts • Capture energy from sunlight and convert into chemical energy in photosynthesis • Contain 2 membranes and chlorophyll Organelle DNA • • • • • • Organelle DNA In chloroplasts and mitochondria Small DNA molecules Maybe descendants of early prokaryotes ----Endosymbiotic theory says these prokaryotic ancestors developed a symbiotic relationship w/ early eukaryotes and resided within---evolving into mitochondria • All cells have a _____________________________and some have a cell wall Cell membrane A. Cell Membrane • Regulates what enters and leaves the cell and also provides _____________________________. • Almost all cell membranes are made of a double layered sheet called a ___________________________-flexible,yet strong barrier • Cell membranes usually have a protein molecule imbedded in the bilayer w/ carbohydrate molecules attached • Called a _________________model • Some of the proteins form channels or pumps to move material across the membranes • Some of the carbs act as ____________________tags Protection and support Phospholipid bilayer Fluid mosaic Chemical id tags B. Cell Walls • In plants,algae,fungi, and many prokaryotes • Lie _______________the cell membrane • Usually porous enough to let water,O2,CO2 and certain other substances to pass through easily • Main function is support and protection • Usually made of fibers of ____________________produced in cell and secreted to surface • Mostly _____________________-tough carb fibers/to withstand gravity Carbohydrate and protein outside cellulose C.Diffusion Through Cell Boundaries • Every cell is in a liquid environment • Cell membrane regulates the movement of cell materials from one side to the other 1.Measuring concentration – • Cytoplasm is a solution of various substances in water _____________of a solution is the mass of solute in given volume of solution---ie. Mass/volume…..If you have 15 g salt in 3 mL water,what is the concentration?------_______….If you have 24 g salt in 2mL water you would have 12 g/mL salt….Which solution is more concentrated?______________ 12 g/mL 5g/mL concentration DIFFUSION Diffusion-Passive Transport-needs no energy moves WITH concentration gradient – • In a solution the particles move constantly,spreading out randomly….tending to move where more concentrated to an area less concentrated…This is called __________________. – ____________________= concentration of a solute is the same throughout a system – does not require energy because random movement if equilibrium is reached,particles keep moving across the membrane,still balancing concentration isotonic Figure 7-17 Osmosis Section 7-3 Higher Concentration of Water Water molecules Cell membrane Lower Concentration of Water Sugar molecules Go to Section: D. Osmosis – Some molecules are too large or too strongly charged to make it across the lipid bilayer----thus impermeable to it – Most membranes are selectively permeable – _____________________is the diffusion of water across a selectively permeable membrane – water moves easily and will move to balance the concentration of a solute,water moving from area of higher to lesser conc. For the WATER – ____________________-same strength of a solute on both sides of a cell membrane osmosis isotonic – more concentrated side of solute is ____________________ – less concentrated side is____________________ – Osmosis exerts a pressure known as ____________________________on the hypertonic side of a membrane….This could results in a cell bursting – Bursting not so much a problem in larger organisms….tend to be in isotonic environments • Osmotic pressure may not allow a plant or bacterial cell to burst , but could weaken the cell wall hypertonic hypotonic Osmotic pressure • Many cells have water channel proteinsaquaporins-allowing water to pass as they are lipid soluble E.Facilitated Diffusion » » » Some molecules,like glucose ,diffuse quickly across due to ________________________ These allow only certain molecules to pass Since it is diffusion it does not require energy and still goes from area of higher to lower concentration » Use channel proteins » Channel proteins provide a tunnel for the substrate to pass across the membrane. Carrier proteins actually bind the substrate then change shape and deposit the substrate on the other side of the membrane VESICULAR TRANSPORT_Endocytosis and Exocytosis • • • Transports larger molecules and even clumps of matter ________________________is the process of taking material inward by enfolding,or pockets In endocytosis ,the pocket breaks loose from the cell membrane and forms a vacuole…large molecules,food and even whole cells can be taken in this way endocytosis 2 examples of endocytosis are – ___________________-extensions of cytoplasm surround a particle and package it in a food vacuole,then the cell engulfs it ---This is how amoebas eat-----is a form of active transport • _______________-Cells use this to take up liquids in the environment—tiny pockets filled w/ liquid form along the cell membrane and pinch off to form vacuoles phagocytosis pinocytosis – ___________________________--releases large amounts from the cell by pinching off or a contractile vacuole as in paramecium--also active transport exocytosis Figure7-20 Active Transport Section 7-3 Molecule to be carried Low Concentration Cell Membrane High Concentration Molecule being carried Low Concentration Cell Membrane High Concentration Energy Go to Section: Energy IV. The Diversity of Cellular Life A. Unicellular Organisms --Single-celled Organisms that do all a living thing would ----They dominate life on earth in terms of numbers B. Multicellular organisms • Multicellular organisms – Made up of many cells – Depend on communication and cooperation between specialized cells ---_______________-cells throughout organism can develop in different ways to perform different tasks Cell specialization – 3 Types of cell junctions – 1) gap junctions-hallow tubes carry out chemical communication-eg.heart tissue – 2)desmosomes-protein filaments create elasticity between skin cells – 3)Tight junctions- adhere closely and are more impermeable • • • • 1. Specialized animal cells – eg. Red blood cells equipped to carry oxygen ;cells specialized to produce proteins produced in pancreas(have many ribosomes and rough ER);muscle cells have actin and myosin cytoskeleton elements for contraction 2. Specialized Plant cells ______________________-are tiny openings on underside of leaves and exchange gases _____________________-regulate gaseous exchanges in stomata,changing shape due to plant’s internal conditions • stomata Guard cells C. Levels of Organization – 1. individual cells – 2. _____________________-group of similar cells w/ particular function – 3. ______________-group of tissues working together – 4. ______________-group of organs working together for particular function • tissue organ Organ system Levels of Organization Section 7- 4 Muscle cell Go to Section: Smooth muscle tissue Stomach Digestive system