Download Reading Guide

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project

Document related concepts

Holliday junction wikipedia , lookup

Agarose gel electrophoresis wikipedia , lookup

Mutation wikipedia , lookup

DNA repair wikipedia , lookup

Eukaryotic transcription wikipedia , lookup

DNA sequencing wikipedia , lookup

Maurice Wilkins wikipedia , lookup

RNA-Seq wikipedia , lookup

Transcriptional regulation wikipedia , lookup

Genome evolution wikipedia , lookup

Promoter (genetics) wikipedia , lookup

Gene expression wikipedia , lookup

Gel electrophoresis of nucleic acids wikipedia , lookup

Silencer (genetics) wikipedia , lookup

Point mutation wikipedia , lookup

Transformation (genetics) wikipedia , lookup

Bisulfite sequencing wikipedia , lookup

Vectors in gene therapy wikipedia , lookup

Genomic library wikipedia , lookup

Real-time polymerase chain reaction wikipedia , lookup

Community fingerprinting wikipedia , lookup

DNA supercoil wikipedia , lookup

Molecular evolution wikipedia , lookup

Molecular cloning wikipedia , lookup

Cre-Lox recombination wikipedia , lookup

Non-coding DNA wikipedia , lookup

Replisome wikipedia , lookup

Nucleic acid analogue wikipedia , lookup

Artificial gene synthesis wikipedia , lookup

Deoxyribozyme wikipedia , lookup

Transcript
Reading Guide: Pratt and Cornely, Chapter 3.1-3.3
1. Define purine, pyrimidine, nucleoside, and nucleotide.
2. From memory, draw AMP and dTTP.
3. What are other functions of nucleotides other than building blocks of DNA and RNA?
4. Draw the DNA strand dAdTdC. Label the 5’ and 3’ ends. Label the phosphodiester bond.
5. Draw the AT base pair and indicate hydrogen bonding. Do the same for the GC base pair.
6. Describe “typical” DNA (B-DNA form) in terms of its major features. How is A-DNA
different?
7. What is the major structural difference between a nucleotide and a deoxynucleotide? What is
the major structural difference between DNA and RNA? What is the major functional difference
between DNA and RNA?
8. True or false: GC rich DNA strands are harder to separate because GC pairs form more Hbonds. Explain.
9. Draw a DNA melting curve for a helix with Tm = 78 oC. Label the y-axis and x-axis.
10. Define denature, renature, and anneal.
11. Draw a schematic for the central dogma of molecular biology, defining replication,
transcription, and translation.
12. Below is the template strand for a small gene. Draw the coding strand, the mRNA produced,
and the polypeptide. (Refer to Table 3.3)
3’-TCCGTAACC-5’
13. What protein is affected by the Cystic Fibrosis gene mutation? How is the protein affected?
14. About how many genes are found in E. coli? In Yeast? In humans?
15. What percentage of the human genome contains genes which encode protein products?
16. Describe two ways in which genes are identified within a genome.
Optional Lecture: Pratt and Cornely, Chapter 3.4
1. What is the goal of DNA sequencing?
2. Why is a DNA primer needed in the Sanger method? What is the role of a DNA polymerase?
What is the role of a ddNTP?
3. How is pyrosequencing used to sequence a DNA strand?
4. If a DNA strand is too long for the Sanger method, how can it be sequenced?
5. What is the goal of the PCR technique?
6. Summarize the steps of the PCR reaction.
7. Define endonuclease and restriction digest.
8. What is recombinant DNA technology? What is a plasmid, and how is it different than a
cloning vector?
9. How are ampR and lacZ used to select for bacteria containing a cloning vector?
10 What is site-directed mutagenesis, and how is it accomplished?
11. What are potential benefits and problems with transgenic crops?
12. What are the major problems facing greater use of human gene therapy?