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Chapter 4- Cells Organisms are composed of one to many microscopic cells Unicellular Multicellular Multicellular organisms are composed of one or more types of tissues Different types of tissues are grouped to form organs The Two Major Types of Cells The Three Domains of Life Domain Bacteria •Prokaryotic cells Domain Archaea Domain Eukarya includes all of the eukaryotic organisms. Domain Eukarya Protists (multiple kingdoms) Kingdom Plantae Kingdom Fungi Kingdom Animalia •Eukaryotic cells Diagram of a plant cell All eukaryotic cells and prokaryotic cells have at least three components in common: Prokaryotic flagella Nucleoid region (DNA) Plasma Ribosomes membrane Cell wall The plasma membrane Capsule Pili The plasma membrane is composed of phospholipids, proteins, and other materials. The cytoplasm consists of the entire region of the cell between the nucleus and plasma membrane The cytosol is the cellular fluid. Ribosomes, are composed of protein and RNA. Cell Membrane Structure and Function The membranes of cells are composed of: Outside cell Hydrophilic head Hydrophobic tail Cytoplasm (inside cell) •The lipids belong to a special category called phospholipids (a) Phospholipid bilayer of membrane Hydrophilic region of protein Phospholipid bilayer Hydrophobic region of protein (b) Fluid mosaic model of membrane The cell membrane is described as selectively permeable because it allows free passage of some materials and not others. -Very small, uncharged molecules can easily pass through the membrane -Larger molecules and ions (molecules with an electrical charge) do not pass through the cell membrane without specific membrane transport proteins. Selective membrane permeability is the basis for osmosis. Osmosis and Water Balance in Cells Osmosis is the diffusion of water across the cell membrane in response to differences in concentrations of solutes (dissolved substances) such as sugars or ions. Diffusion Water, like many substances, will move from where it is more concentrated to where it is less concentrated. If the concentration of solutes inside and outside the cell is the same, then the cell is osmotically balanced. Osmosis and Water Balance in Cells If the solute concentration is lower outside a cell than inside, Prokaryotes, protists, fungi, and plants have cell walls that limit the increase of cell volume through the uptake of water. If the concentration of solutes outside the cells is higher than inside, water leaves the cell and the cytoplasm shrinks. Eukaryotic cells share some features that prokaryotes lack All eukaryotic cells have a: The nucleus is the major site of genetic information (DNA) storage in eukaryotic cells. The nuclear envelope Ribosomes Chromatin Nuclear envelope Nucleolus Pore Nuclei also contain nucleoli (nucleolus, sing.), Eukaryotic nuclear DNA is combined with proteins to form chromosomes. •Many of the membranous organelles in the cell belong to the endomembrane system The endoplasmic reticulum (ER) Nuclear envelope Rough ER •The “roughness” of the rough ER is due to ribosomes that stud the outside of the ER membrane Ribosomes Rough ER Ribosomes Smooth ER Small globular structures composed of protein and RNA that generate proteins from amino acids. 4 Transport vesicle buds off Ribosome 3 After the rough ER synthesizes a molecule it packages the molecule into transport vesicles Secretory protein inside transport vesicle Protein 1 Polypeptide 2 Rough ER Smooth ER lacks the surface ribosomes of rough ER Nuclear envelope Ribosomes Rough ER The Golgi apparatus Works in partnership with ER The Golgi apparatus of a cell consists of 1 or more Golgi bodies. The cytoskeleton consists of three major types of long, thin, protein fibers: Flagella and Cilia Eukaryotic flagella typically contain a pair of single microtubules, surrounded by a cylinder of nine paired microtubules (9 + 2 arrangement). Smooth ER Mitochondria Each mitochondrion is enclosed by an envelope composed of an outer membrane and a highly folded inner membrane. Peroxisomes -surrounded by single membrane Plant cells Plant cells are distinguished from most other eukaryotic cells by: Components of cell walls: Cellulose - the most abundant organic compound on Earth Cellulose is a polymer of glucose units, but the glucose monomers are linked together in different orientation. Non-cellulose components of plant cell walls Plant cell walls also contain other polysaccharides such as: Hemicellulose is a polymer of glucose and other sugars. Pectin Primary and Secondary Plant Cell Walls Plant cells that are still growing have only a single cell walllayer, or primary cell wall. Secondary cell walls Lignin may be added to the walls of water-conducting cells, so mature cell walls can no longer stretch. Plasmodesmata Large ions and molecules can be transported between the cells. Chloroplasts Leaves and other green parts of plants contain organelles called chloroplasts. The more general term plastid is used to describe Plant and algal cells contain from one to many plastids per cell. Plant cells are enclosed by an envelope composed of 2 membranes. The watery stroma contains other enzymes for photosynthesis. Other types of plastids Amyloplasts Chromoplasts Central vacuole Study outline for Chapter 4-Cells --Understand cell membrane structure and function- What are membranes of cells composed of? -Know the structure of phospholipid bilayer of a membrane -Understand the concept of selectively permeable -Know examples of what can pass easily through membranes and substances that will not pass without specific membrane transport proteins -Understand osmosis and water balance in cells -Know terms-osmosis, diffusion, isotonic, hypotonic, hypertonic, contractile vacuole, plasmolysis -Know the features that eukaryotic cells share but prokaryotes lack -Know structure and function-nucleus, nuclear envelope, nucleolus, chromosomes -Know the membranous organelles that belong to the endomembrane system-ER (rough and smooth), ribosomes, Golgi apparatus, Golgi body, cisternae -Know the three major types of fibers in the cytoskeleton-microtubules, microfilaments, intermediate filaments -Understand flagella and cilia- 9 + 2 arrangement -Know structure and function of mitochondria and peroxisomes Plant cells are distinguished from most other eukaryotic cells by which 4 cellular features? -Know functions of cellulose cell walls -Understand the structure of cellulose -Know the structure and function of non-cellulose components of plant cell walls: hemicellulose, pectin -Know the difference between primary and secondary plant cell walls -Understand the structure and function of plasmodesmata -Understand structure and function of different types of plastids-chloroplast, chromoplast, leucoplast (amyloplast) -Know terms-thylakoid, stroma -Understand structure and function of central vacuole