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How do digestive enzymes work and where are they produced? Baseline (Flightpath D): To be able to state that enzymes are used in digestion to break down food molecules and identify that carbohydrases break down carbohydrates, proteases break down proteins, and lipases break down lipids. Further (Flightpath C&B ): To be able to explain why enzymes are needed for digestion. For each food molecule, name the enzyme that acts on it, where it is produced, and which products are formed. Challenge Flightpath A):to be able to suggest how to test for substrates and products in a model gut and make a prediction with a clearly structured scientific explanation. Write a question that has one of the key words as the answer • • • • • • • • • • enzyme catalyst active site substrate lock and key model denature digestion carbohydrate lipid protein Reaction rates • Two people walk to school. Sam’s journey is 1 km and it takes him 20 minutes. Ruby walks 0.5 km in 15 minutes. Ask students to work out who walked at the fastest mean rate? Starch molecules – large and branched Amylase – digestive enzyme Breaks down starch to Maltose Still too big! Maltase in the small intestine breaks down maltose to glucose units Readily absorbed. Proteins – large and branched Amino acid Amino acid Amino acid Amino acid Trypsin from the pancreas Amino acid Amino acid Amino acid Amino acid Breaks down proteins into peptides. Amino acid Amino acid Amino acid Amino acid Amino acid Amino acid Amino acid Amino acid Amino acid Amino acid Still too big! Amino acid Peptidase from the small intestine Amino acid Amino acid Amino acid Amino acid Amino acid Amino acid Fats & Oils Fatty acids and glycerol which is easily absorbed. Bile from the liver mixes with the ‘fat’ to make an emulsion Lipase from the pancreas breaks down the fats to... What are enzymes made of? Enzymes are protein molecules, and so are made up of amino acids. Most enzymes contain between 100 and 1,000 amino acids. These amino acids are joined together in a long chain, which is folded to produce a unique 3D structure. Why is shape important? The shape of an enzyme is very important because it has a direct effect on how it catalyzes a reaction. Why do enzymes have different shapes? An enzyme’s shape is determined by the sequence of amino acids in its structure, and the bonds which form between the atoms of those molecules. Different types of enzymes have different shapes and functions because the order and type of amino acids in their structure is different. Why are enzymes so specific? Enzymes are very specific about which reactions they catalyze. Only molecules with exactly the right shape will bind to the enzyme and react. These are the reactant, or substrate, molecules. The part of the enzyme to which the reactant binds is called the active site. This is a very specific shape and the most important part of the enzyme. Enzymes: true or false? What happens at the active site? In the same way that a key fits into a lock, so a substrate is thought to fit into an enzyme’s active site. The enzyme is the lock, and the reactant is the key. ↔ + enzyme + reactant ↔ ↔ enzyme-reactant complex + ↔ enzyme + products The lock and key model Digestion in the stomach When food enters the stomach it stimulates the secretion of hydrochloric acid (HCl) from the stomach wall. HCl increases the acidity of the stomach to about pH2 – the optimum pH for stomach enzymes. oesophagus mucus cells gastric gland parietal cells (acid-producing) duodenum Match the reactant Digestion in the small intestine Digestive enzymes found in the small intestine are damaged by a strongly acidic pH. How does the body avoid this problem? The liver produces bile (an alkali), which is stored in the gall bladder and released into the small intestine. hepatic duct Bile neutralizes the acidic contents coming from the stomach, creating the alkaline gall environment that the intestinal bladder enzymes need to work. pancreas duodenum bile duct Factors affecting enzymes The rate of enzyme–catalyzed reactions depends on several factors. What are some of these? Factors that affect the rate of a reaction include: temperature substrate concentration pH surface area enzyme concentration pressure. All enzymes work best at only one particular temperature and pH: this is called the optimum. Different enzymes have different optimum temperatures and pH values. Enzyme inhibitors Factors affecting enzymes If the temperature and pH changes sufficiently beyond an enzyme’s optimum, the shape of the enzyme irreversibly changes. This affects the shape of the active site and means that the enzyme will no longer work. When this happens the enzyme is denatured. heat pH normal denatured Enzymes in the home How many items are made using enzymes? • Exam question Mark Scheme a) (i) stomach (ii) small intestine B) 1 1 salivary glands stomach pancreas small intestine amylase ✓ ✕ ✓ ✓ lipase ✕ ✕ ✓ ✓ protease ✕ ✓ ✓ ✓ 1 mark per correct row or if no correct row max 1 mark for any one correct column (c) enzyme / protease / pepsin most effective in acid conditions / low pH accept optimum / correct pH do not accept ref to incorrectly named enzymes ignore killing bacteria ignore acid breaks down food • (d) Enzyme Breakdown products